The distribution of feline immunodeficiency virus in tissue compartments of feral domestic cats
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Prevalence and subtype studies of feline immunodeficiency virus (FIV) in the domestic cat (Felis catus) have been conducted thoroughly. However, the intrahost dynamics of FIV have been less well studied. Here, we determine the diversity of FIV env V3–V6 sequences isolated from multiple tissues of naturally infected feral cats. Using nested PCR, FIV was amplified from non-lymphoid tissues from eight of sixteen cats that had amplifiable proviral FIV DNA in the popliteal lymph node. In general, we found low intrahost FIV diversity, but there was evidence of tissue compartmentalization in one cat.
Feline immunodeficiency virus is a lentivirus, closely related to human immunodeficiency virus (HIV), which can cause immunodeficiency in F. catus . FIV has been detected in a number of tissue types, including lymphoid tissue, central nervous system (CNS), bone marrow, intestine, liver and lung [2, 4, 15, 22].
Compartmentalization is the restriction of virus movement between different tissues...
KeywordsFeline Immunodeficiency Virus Proviral Load Feline Immunodeficiency Virus Infection High Proviral Load Random Leaf
The authors wish to thank Dr. Emma Marks for editing. Sequencing was done at the Allan Wilson Centre, Massey University, Auckland. Feral cats were provided by the New Zealand Department of Conservation. This research was funded by the Allan Wilson Centre for Molecular Ecology and Evolution, and The University of Auckland. Jessica Hayward was supported by a University of Auckland Doctoral Scholarship.
- 10.Itescu S, Simonelli PF, Winchester RJ, Ginsberg HS (1994) Human immunodeficiency virus type 1 strains in the lungs of infected individuals evolve independently from those in peripheral blood and are highly conserved in the C-terminal region of the envelope V3 loop. Proc Natl Acad Sci USA 91:11378–11382CrossRefPubMedGoogle Scholar
- 11.Kemal KS, Foley B, Burger H, Anastos K, Minkoff H, Kitchen C, Philpott SM, Gao W, Robison E, Holman S, Dehner C, Beck S, Meyer WA III, Landay A, Kovacs A, Bremer J, Weiser B (2003) HIV-1 in genital tract and plasma of women: compartmentalization of viral sequences, coreceptor usage, and glycosylation. Proc Natl Acad Sci USA 100:12972–12977CrossRefPubMedGoogle Scholar
- 12.Korber BTM, Kunstman KJ, Patterson BK, Furtado M, McEvilly MM, Levy R, Wolinsky SM (1994) Genetic differences between blood- and brain-derived viral sequences from human immunodeficiency virus type 1-infected patients: evidence of conserved elements in the V3 region of the envelope protein of brain-derived sequences. J Virol 68:7467–7481PubMedGoogle Scholar
- 21.Rodrigo AG, Hanley EW, Goracke PC, Learn GH (2001) Sampling and processing HIV molecular sequences: a computational evolutionary biologist’s perspective. In: Rodrigo AG, Learn GH (eds) Computational and evolutionary analysis of HIV molecular sequences. Kluwer, Massachusetts, pp 1–17Google Scholar
- 22.Ryan G, Klein D, Knapp E, Hosie MJ, Grimes T, Mabruk MJ, Jarrett O, Callanan JJ (2003) Dynamics of viral and proviral loads of feline immunodeficiency virus within the feline central nervous system during the acute phase following intravenous infection. J Virol 77:7477–7485CrossRefPubMedGoogle Scholar
- 23.Shankarappa R, Margolick JB, Gange SJ, Rodrigo AG, Upchurch D, Farzadegan H, Gupta P, Rinaldo CR, Learn GH, He X, Huang XL, Mullins JI (1999) Consistent viral evolutionary changes associated with the progression of human immunodeficiency virus type 1 infection. J Virol 73:10489–10502PubMedGoogle Scholar
- 28.Swofford DL (2002) PAUP*. Phylogenetic analysis using parsimony (*and other methods), version 4. Sinauer Associates, SunderlandGoogle Scholar