Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection
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Eukaryotic translation elongation factor 1A (eEF1A) has been shown to interact with both the viral RNA-dependent RNA polymerase and the 3′-terminal genomic RNA of tobacco mosaic virus (TMV). In this study, we demonstrated that the down-regulation of eEF1A mRNA levels by virus-induced gene silencing using potato virus X vector dramatically reduced the accumulation of TMV RNA and the spread of TMV infection. The translation activity of the eEF1A-silenced Nicotiana benthamiana leaves was not severely affected. Collectively, these results suggest an essential role of eEF1A in TMV infection.
KeywordsWest Nile Virus Tobacco Mosaic Virus Tobacco Mosaic Virus Infection Fluorescent Focus Turnip Yellow Mosaic Virus
We thank Dr. D. C. Baulcombe for providing PVX vector and N. benthamiana line 16c and Dr. W. O. Dawson for p30B:GFP. This work was supported by a grant-in-aid (12052207) for scientific research on priority area from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and a grant-in-aid (13854004 and 17780033) from the Japan Society for the Promotion of Science.