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Archives of Virology

, Volume 155, Issue 2, pp 263–268 | Cite as

Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection

  • Yasuyuki Yamaji
  • Keitaro Sakurai
  • Koji Hamada
  • Ken Komatsu
  • Johji Ozeki
  • Akiko Yoshida
  • Atsushi Yoshii
  • Takumi Shimizu
  • Shigetou Namba
  • Tadaaki Hibi
Brief Report

Abstract

Eukaryotic translation elongation factor 1A (eEF1A) has been shown to interact with both the viral RNA-dependent RNA polymerase and the 3′-terminal genomic RNA of tobacco mosaic virus (TMV). In this study, we demonstrated that the down-regulation of eEF1A mRNA levels by virus-induced gene silencing using potato virus X vector dramatically reduced the accumulation of TMV RNA and the spread of TMV infection. The translation activity of the eEF1A-silenced Nicotiana benthamiana leaves was not severely affected. Collectively, these results suggest an essential role of eEF1A in TMV infection.

Keywords

West Nile Virus Tobacco Mosaic Virus Tobacco Mosaic Virus Infection Fluorescent Focus Turnip Yellow Mosaic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Dr. D. C. Baulcombe for providing PVX vector and N. benthamiana line 16c and Dr. W. O. Dawson for p30B:GFP. This work was supported by a grant-in-aid (12052207) for scientific research on priority area from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and a grant-in-aid (13854004 and 17780033) from the Japan Society for the Promotion of Science.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Yasuyuki Yamaji
    • 1
  • Keitaro Sakurai
    • 1
  • Koji Hamada
    • 1
  • Ken Komatsu
    • 1
  • Johji Ozeki
    • 1
  • Akiko Yoshida
    • 1
  • Atsushi Yoshii
    • 1
  • Takumi Shimizu
    • 1
  • Shigetou Namba
    • 1
  • Tadaaki Hibi
    • 2
  1. 1.Laboratory of Plant Pathology, Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan
  2. 2.Faculty of Bioscience and Applied ChemistryHosei UniversityTokyoJapan

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