Archives of Virology

, Volume 155, Issue 2, pp 263–268 | Cite as

Significance of eukaryotic translation elongation factor 1A in tobacco mosaic virus infection

  • Yasuyuki YamajiEmail author
  • Keitaro Sakurai
  • Koji Hamada
  • Ken Komatsu
  • Johji Ozeki
  • Akiko Yoshida
  • Atsushi Yoshii
  • Takumi Shimizu
  • Shigetou Namba
  • Tadaaki Hibi
Brief Report


Eukaryotic translation elongation factor 1A (eEF1A) has been shown to interact with both the viral RNA-dependent RNA polymerase and the 3′-terminal genomic RNA of tobacco mosaic virus (TMV). In this study, we demonstrated that the down-regulation of eEF1A mRNA levels by virus-induced gene silencing using potato virus X vector dramatically reduced the accumulation of TMV RNA and the spread of TMV infection. The translation activity of the eEF1A-silenced Nicotiana benthamiana leaves was not severely affected. Collectively, these results suggest an essential role of eEF1A in TMV infection.


West Nile Virus Tobacco Mosaic Virus Tobacco Mosaic Virus Infection Fluorescent Focus Turnip Yellow Mosaic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank Dr. D. C. Baulcombe for providing PVX vector and N. benthamiana line 16c and Dr. W. O. Dawson for p30B:GFP. This work was supported by a grant-in-aid (12052207) for scientific research on priority area from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and a grant-in-aid (13854004 and 17780033) from the Japan Society for the Promotion of Science.


  1. 1.
    Ahlquist P, Noueiry AO, Lee WM, Kushner DB, Dye BT (2003) Host factors in positive-strand RNA virus genome replication. J Virol 77:8181–8186CrossRefPubMedGoogle Scholar
  2. 2.
    Andersen GR, Nissen P, Nyborg J (2003) Elongation factors in protein biosynthesis. Trends Biochem Sci 28:434–441CrossRefPubMedGoogle Scholar
  3. 3.
    Axelos M, Bardet C, Liboz T, Van Thai AL, Curie C, Lescure B (1989) The gene family encoding the Arabidopsis thaliana translation elongation factor EF-1α: molecular cloning, characterization and expression. Mol Gen Genet 219:106–112CrossRefPubMedGoogle Scholar
  4. 4.
    Blumenthal T, Carmichael GG (1979) RNA replication: function and structure of Qβ-replicase. Annu Rev Biochem 48:525–548CrossRefPubMedGoogle Scholar
  5. 5.
    Browning KS (1996) The plant translational apparatus. Plant Mol Biol 32:107–144CrossRefPubMedGoogle Scholar
  6. 6.
    Burch-Smith TM, Anderson JC, Martin GB, Dinesh-Kumar SP (2004) Applications and advantages of virus-induced gene silencing for gene function studies in plants. Plant J 39:734–746CrossRefPubMedGoogle Scholar
  7. 7.
    Canto T, Palukaitis P (2002) Novel N gene-associated, temperature-independent resistance to the movement of tobacco mosaic virus vectors neutralized by a cucumber mosaic virus RNA1 transgene. J Virol 76:12908–12916CrossRefPubMedGoogle Scholar
  8. 8.
    Condeelis J (1995) Elongation factor 1α, translation and the cytoskeleton. Trends Biochem Sci 20:169–170CrossRefPubMedGoogle Scholar
  9. 9.
    Davis WG, Blackwell JL, Shi PY, Brinton MA (2007) Interaction between the cellular protein eEF1A and the 3′-terminal stem-loop of West Nile virus genomic RNA facilitates viral minus-strand RNA synthesis. J Virol 81:10172–10187CrossRefPubMedGoogle Scholar
  10. 10.
    De Nova-Ocampo M, Villegas-Sepulveda N, del Angel RM (2002) Translation elongation factor-1α, La, and PTB interact with the 3′ untranslated region of dengue 4 virus RNA. Virology 295:337–347CrossRefPubMedGoogle Scholar
  11. 11.
    Durso NA, Cyr RJ (1994) A calmodulin-sensitive interaction between microtubules and a higher plant homolog of elongation factor-1 α. Plant Cell 6:893–905CrossRefPubMedGoogle Scholar
  12. 12.
    Edmonds BT, Murray J, Condeelis J (1995) pH regulation of the F-actin binding properties of Dictyostelium elongation factor 1 α. J Biol Chem 270:15222–15230CrossRefPubMedGoogle Scholar
  13. 13.
    Gross SR, Kinzy TG (2005) Translation elongation factor 1A is essential for regulation of the actin cytoskeleton and cell morphology. Nat Struct Mol Biol 12:772–778CrossRefPubMedGoogle Scholar
  14. 14.
    Johnson CM, Perez DR, French R, Merrick WC, Donis RO (2001) The NS5A protein of bovine viral diarrhoea virus interacts with the a subunit of translation elongation factor-1. J Gen Virol 82:2935–2943PubMedGoogle Scholar
  15. 15.
    Kawakami S, Watanabe Y, Beachy RN (2004) Tobacco mosaic virus infection spreads cell to cell as intact replication complexes. Proc Natl Acad Sci USA 101:6291–6296CrossRefPubMedGoogle Scholar
  16. 16.
    Kou YH, Chou SM, Wang YM, Chang YT, Huang SY, Jung MY, Huang YH, Chen MR, Chang MF, Chang SC (2006) Hepatitis C virus NS4A inhibits cap-dependent and the viral IRES-mediated translation through interacting with eukaryotic elongation factor 1A. J Biomed Sci 13:861–874CrossRefPubMedGoogle Scholar
  17. 17.
    Lai MM (1998) Cellular factors in the transcription and replication of viral RNA genomes: a parallel to DNA-dependent RNA transcription. Virology 244:1–12CrossRefPubMedGoogle Scholar
  18. 18.
    Li Z, Pogany J, Panavas T, Xu K, Esposito AM, Kinzy TG, Nagy PD (2009) Translation elongation factor 1A is a component of the tombusvirus replicase complex and affects the stability of the p33 replication co-factor. Virology 385:245–260CrossRefPubMedGoogle Scholar
  19. 19.
    Liu JZ, Blancaflor EB, Nelson RS (2005) The tobacco mosaic virus 126-kilodalton protein, a constituent of the virus replication complex, alone or within the complex aligns with and traffics along microfilaments. Plant Physiol 138:1853–1865CrossRefPubMedGoogle Scholar
  20. 20.
    Matsuda D, Dreher TW (2004) The tRNA-like structure of Turnip yellow mosaic virus RNA is a 3′-translational enhancer. Virology 321:36–46CrossRefPubMedGoogle Scholar
  21. 21.
    Matsuda D, Yoshinari S, Dreher TW (2004) eEF1A binding to aminoacylated viral RNA represses minus strand synthesis by TYMV RNA-dependent RNA polymerase. Virology 321:47–56CrossRefPubMedGoogle Scholar
  22. 22.
    Nishikiori M, Dohi K, Mori M, Meshi T, Naito S, Ishikawa M (2006) Membrane-bound tomato mosaic virus replication proteins participate in RNA synthesis and are associated with host proteins in a pattern distinct from those that are not membrane bound. J Virol 80:8459–8468CrossRefPubMedGoogle Scholar
  23. 23.
    Qanungo KR, Shaji D, Mathur M, Banerjee AK (2004) Two RNA polymerase complexes from vesicular stomatitis virus-infected cells that carry out transcription and replication of genome RNA. Proc Natl Acad Sci USA 101:5952–5957CrossRefPubMedGoogle Scholar
  24. 24.
    Ruiz MT, Voinnet O, Baulcombe DC (1998) Initiation and maintenance of virus-induced gene silencing. Plant Cell 10:937–946CrossRefPubMedGoogle Scholar
  25. 25.
    Shivprasad S, Pogue GP, Lewandowski DJ, Hidalgo J, Donson J, Grill LK, Dawson WO (1999) Heterologous sequences greatly affect foreign gene expression in tobacco mosaic virus-based vectors. Virology 255:312–323CrossRefPubMedGoogle Scholar
  26. 26.
    Thivierge K, Cotton S, Dufresne PJ, Mathieu I, Beauchemin C, Ide C, Fortin MG, Laliberté JF (2008) Eukaryotic elongation factor 1A interacts with Turnip mosaic virus RNA-dependent RNA polymerase and VPg-Pro in virus-induced vesicles. Virology 377:216–225CrossRefPubMedGoogle Scholar
  27. 27.
    Voinnet O, Vain P, Angell S, Baulcombe DC (1998) Systemic spread of sequence-specific transgene RNA degradation is initiated by localized introduction of ectopic promoterless DNA. Cell 95:177–187CrossRefPubMedGoogle Scholar
  28. 28.
    Yamaji Y, Kobayashi T, Hamada K, Sakurai K, Yoshii A, Suzuki M, Namba S, Hibi T (2006) In vivo interaction between Tobacco mosaic virus RNA-dependent RNA polymerase and host translation elongation factor 1A. Virology 347:100–108CrossRefPubMedGoogle Scholar
  29. 29.
    Zeenko VV, Ryabova LA, Spirin AS, Rothnie HM, Hess D, Browning KS, Hohn T (2002) Eukaryotic elongation factor 1A interacts with the upstream pseudoknot domain in the 3′ untranslated region of tobacco mosaic virus RNA. J Virol 76:5678–5691CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Yasuyuki Yamaji
    • 1
    Email author
  • Keitaro Sakurai
    • 1
  • Koji Hamada
    • 1
  • Ken Komatsu
    • 1
  • Johji Ozeki
    • 1
  • Akiko Yoshida
    • 1
  • Atsushi Yoshii
    • 1
  • Takumi Shimizu
    • 1
  • Shigetou Namba
    • 1
  • Tadaaki Hibi
    • 2
  1. 1.Laboratory of Plant Pathology, Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan
  2. 2.Faculty of Bioscience and Applied ChemistryHosei UniversityTokyoJapan

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