Archives of Virology

, Volume 155, Issue 2, pp 243–245 | Cite as

Complete sequence analysis of rice transitory yellowing virus and its comparison to rice yellow stunt virus

  • Akihiro Hiraguri
  • Hiroyuki Hibino
  • Takaharu Hayashi
  • Takumi Shimizu
  • Tamaki Uehara-Ichiki
  • Toshihiro Omura
  • Takahide SasayaEmail author
Brief Report


Rice transitory yellowing virus (RTYV), a member of the genus Nucleorhabdovirus, is closely related to or synonymous with rice yellow stunt virus (RYSV). To clarify the relationship between RTYV and RYSV, we determined the nucleotide sequence of the RTYV genome. The RTYV genome consists of 14,029 nucleotides. The overall nucleotide identity between RTYV and RYSV was 98.5%, and the deduced amino acid sequence identities between the seven genes in RTYV and RYSV ranged from 82.3 to 99.7%. The sequence information from RTYV revealed that these two viruses should be categorized as members of the same species rather than distinct species.


Rice Plant Deduce Amino Acid Sequence Tiller Number Nucleotide Sequence Identity Plant Hopper 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was supported by the Program for Promotion of Basic Research Activities for Innovation Bioscience (PROBRAIN) of Japan.


  1. 1.
    Chen MJ, Shikata E (1968) Electron microscopy of virus-like particles associated with transitory yellowing virus-infected rice plants in Taiwan. Plant Prot Bull (Taiwan) 10:19–28Google Scholar
  2. 2.
    Chen SX, Yuan YL, King DD, Chen KY, Lin RT, Kao TM (1979) Studies on the development and epiphytotris of the rice yellow stunt disease. Acta Phytopathol Sin 9:41–54Google Scholar
  3. 3.
    Chiu RJ, Jean JH, Chen MH, Lo TC (1968) Transmission of transitory yellowing virus of rice by two leafhoppers. Phytopathology 58:740–745Google Scholar
  4. 4.
    Chiu RJ, Lo TC, Pi CT, Chen MH (1965) Transitory yellowing of rice and its transmission by the leafhopper Nephotettix apicalis apicalis (Motsch.). Bot Bull Acad Sin 6:1–18Google Scholar
  5. 5.
    Chiu RJ, Hsu YH, Chen MJ, Chen CC, Lee RCR, Lin MC, Lin SM, Kuo TT (1990) Purification and partial characterization of rice transitory yellowing virus. Phytopathology 80:777–783CrossRefGoogle Scholar
  6. 6.
    Fan H, Zeng DS, Ye BJ, Lu BC (1965) The preliminary investigation on rice yellow stunt disease in Guangdong. Plant Prot 3:143–145Google Scholar
  7. 7.
    Fang RX, Pang Z, Xu BY, Mang KQ, Li AM, Qin WS, Chen SX (1992) Partial purification of rice yellow stunt virus and characterization of virus structural proteins and genome. Chin J Viol 8:62–68Google Scholar
  8. 8.
    Fang RX, Wang Q, Xu BY, Pang Z, Zhu HT, Mang KQ, Gao DM, Qin WS, Chua NH (1994) Structure of the nucleocapsid protein gene of rice yellow stunt rhabdovirus. Virology 204:367–375CrossRefPubMedGoogle Scholar
  9. 9.
    Finke S, Cox JH, Conzelmann KK (2000) Differential transcription attenuation of rabies virus genes by intergenic regions: generation of recombinant viruses overexpressing the polymerase gene. J Virol 74:7261–7269CrossRefPubMedGoogle Scholar
  10. 10.
    Futan University (1976) Electron microscopy on the pathogens of the rice yellow stunt disease. Kexue Tongbao 21:551–552Google Scholar
  11. 11.
    Gao DM, Qin WS, Li AM, Cheng SX (1993) Characterization of mainland China rice yellow stunt disease and Taiwan rice transitory yellowing disease by serological identification. Virol Sin 8:177–180Google Scholar
  12. 12.
    Hayashi T, Minobe Y (1985) Protein composition of rice transitory yellowing virus. Microbiol Immunol 29:169–172PubMedGoogle Scholar
  13. 13.
    Hayashi T, Minobe Y (1987) Serological studies on rice transitory yellowing virus (RTYV) in rice plants and green leafhoppers. JARQ 21:90–95Google Scholar
  14. 14.
    Huang Y, Zhao H, Luo Z, Chen X, Fang RX (2003) Novel structure of the genome of Rice yellow stunt virus: identification of the gene 6-encoded virion protein. J Gen Virol 84:2259–2264CrossRefPubMedGoogle Scholar
  15. 15.
    Huang YW, Geng YF, Ying XB, Chen XY, Fang RX (2005) Identification of a movement protein of Rice yellow stunt rhabdovirus. J Virol 79:2108–2114CrossRefPubMedGoogle Scholar
  16. 16.
    Inoue H, Omura T, Hayashi T, Morinaka T, Putta M, Chettanachit D, Parejarearn A, Disthaporn S, Kadkao S (1986) Rice transitory yellowing virus. Virus disease of Rice and Legumes in the Tropics. Tech Bull Trop Agr Res Cent Japan 21:65–77Google Scholar
  17. 17.
    Luo Z, Chen X, Gao D, Fang R (1998) The gene 4 of rice yellow stunt rhabdovirus encodes the matrix protein. Virus Genes 16:277–280CrossRefPubMedGoogle Scholar
  18. 18.
    Luo ZL, Fang RX (1998) Structure analysis of the rice yellow stunt rhabdovirus glycoprotein gene and its mRNA. Arch Virol 143:2453–2459CrossRefPubMedGoogle Scholar
  19. 19.
    Shikata E (1989) Rice transitory yellowing nucleorhabdovirus. In: Brunt AA, Crabtree K, Dallwitz MJ, Gibbs AJ, Watson L (eds) Viruses of plants. Descriptions and lists from the VIDE database. CAB INTERNATIONAL, Wallingford, pp 1101–1102Google Scholar
  20. 20.
    Shikata E, Chen MJ (1969) Electron microscopy of rice transitory yellowing virus. J Virol 3:261–264PubMedGoogle Scholar
  21. 21.
    Shikata E (1972) Rice transitory yellowing. CMI/AAB description of Plant Virus No 100Google Scholar
  22. 22.
    Zhu HT, Chen XY, Luo ZL, Fang RX, Gao DM (1997) Nucleotide sequence of the rice yellow stunt rhabdovirus gene 2. Chin J Virol 13:369–375Google Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Akihiro Hiraguri
    • 1
  • Hiroyuki Hibino
    • 1
  • Takaharu Hayashi
    • 1
  • Takumi Shimizu
    • 1
  • Tamaki Uehara-Ichiki
    • 1
  • Toshihiro Omura
    • 1
  • Takahide Sasaya
    • 1
    Email author
  1. 1.Research Team for Vectorborne DiseasesNational Agricultural Research CenterTsukubaJapan

Personalised recommendations