Archives of Virology

, Volume 155, Issue 2, pp 159–167 | Cite as

Molecular characterization of the VP1, VP2, VP4, VP6, NSP1 and NSP2 genes of bovine group B rotaviruses: identification of a novel VP4 genotype

  • Souvik GhoshEmail author
  • N. Kobayashi
  • S. Nagashima
  • M. Chawla-Sarkar
  • T. Krishnan
  • B. Ganesh
  • T. N. Naik
Original Article


Studies on bovine group B rotaviruses (GBRs) are limited. To date, only the VP6 gene of a single bovine GBR strain and the VP7 and NSP5 genes of a few bovine GBR strains have been sequenced and analyzed. In the present study, using a single-primer amplification method, we have determined the full-length nucleotide sequences of the VP1, VP2, VP4, VP6, NSP1 and NSP2 genes of three bovine GBR strains from eastern India. In all six of these genes, the bovine GBR strains shared high genetic relatedness among themselves but exhibited high genetic diversity with cognate genes of human, murine and ovine GBRs. Interestingly, as with group A rotaviruses, the bovine GBR VP1, VP2, VP6 and NSP2 genes appeared to be more conserved than the VP4 and NSP1 genes among strains of different species. The present study provides important insights into the genetic makeup and diversity of bovine GBRs, and also identifies a novel GBR VP4 genotype.


NSP2 Gene Strain DB176 Sequence Identity Cutoff Strain KB63 High Genetic Relatedness 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The study was supported in part by a Grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (Grant number 20-08463) and the Program of Founding Research Centers for Emerging and Reemerging Infectious Diseases (Okayama University-National Institute of Cholera and Enteric Diseases, India).

Supplementary material

705_2009_555_MOESM1_ESM.pdf (280 kb)
Supplementary material 1 (PDF 280 kb)


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Souvik Ghosh
    • 1
    Email author
  • N. Kobayashi
    • 1
  • S. Nagashima
    • 2
  • M. Chawla-Sarkar
    • 3
  • T. Krishnan
    • 3
  • B. Ganesh
    • 3
  • T. N. Naik
    • 4
  1. 1.Department of HygieneSapporo Medical University School of MedicineSapporoJapan
  2. 2.Division of Virology, Department of Infection and ImmunityJichi Medical University School of MedicineTochigiJapan
  3. 3.Division of VirologyNational Institute of Cholera and Enteric DiseasesKolkataIndia
  4. 4.School of BiologyNational Institute of Science Education and ResearchBhubaneswarIndia

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