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Archives of Virology

, Volume 155, Issue 1, pp 107–110 | Cite as

Readaptation of a low-virulence influenza H9 escape mutant in mice: the role of changes in hemagglutinin as revealed by site-specific mutagenesis

  • N. A. Ilyushina
  • I. A. Rudneva
  • A. M. Khalenkov
  • T. A. Timofeeva
  • P. S. Krylov
  • R. G. Webster
  • N. V. Kaverin
Brief Report

Abstract

In our earlier studies, we showed that an escape mutant of mouse-adapted H9N2 influenza virus carrying a T198N amino acid change in heamagglutinin (HA) has a lowered virulence for mice. The readaptation of this mutant to mice was associated with N198S or N198D reverse mutations. In this study, single-gene reassortants having HA gene of the wild-type virus, its low-virulence escape mutant, or a readapted variant were generated by site-specific mutagenesis and assayed for virulence. The results showed that antibody-selected mutations in the HA of H9 influenza virus can decrease mortality and virus accumulation in mouse lungs, though not in nasal turbinates, and the effect may be compensated by reverse mutations in the course of passaging.

Keywords

Influenza Virus Amino Acid Change Virus Accumulation Escape Mutant Swine Influenza Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by Contract HHSN266200700005C with the National Institute of Allergy and Infectious Diseases, by the American Lebanese Syrian Associated Charities (ALSAC), by Russian Federation For Basic Research grant #07-04-00005, and by NATO Collaborative Linkage Grant 979155.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • N. A. Ilyushina
    • 1
    • 2
  • I. A. Rudneva
    • 1
  • A. M. Khalenkov
    • 2
  • T. A. Timofeeva
    • 1
  • P. S. Krylov
    • 1
  • R. G. Webster
    • 2
    • 3
  • N. V. Kaverin
    • 1
  1. 1.The D.I. Ivanovsky Institute of VirologyMoscowRussia
  2. 2.Division of Virology, Department of Infectious DiseasesSt. Jude Children’s Research HospitalMemphisUSA
  3. 3.Department of PathologyUniversity of TennesseeMemphisUSA

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