An assessment of molecular variability and recombination patterns in South African isolates of Potato virus Y
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The coat protein (CP) gene of 75 South African Potato virus Y (PVY) isolates was amplified using reverse-transcriptase polymerase chain reaction (RT-PCR). The resulting cDNA products were cloned and sequenced. These sequences were used to identify the strains to which the isolates belonged. Some, when compared to reference sequences, belonged to the PVYN and PVYO strains. A number of isolates were found to demonstrate significant homology to PVYN strains from China. A large number of South African isolates possessed CP sequences showing evidence of recombination between PVYN and PVYO strains, similar to those of PVYNTN isolates. Multiplex RT-PCR analysis allowed further differentiation of PVYO isolates and revealed that the majority were of the PVYN-Wilga strain. It was deduced that the most likely way in which these isolates reached South Africa was via the importation of infected material.
KeywordsCoat Protein Coat Protein Gene Wheat Streak Mosaic Virus Coat Protein Sequence Coat Protein Gene Sequence
The authors wish to thank Emcee Gagiano, Anél Espach and Marieta Botha for cultivation and distribution of plant material. The financial support of Potatoes South Africa is gratefully acknowledged.
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