Archives of Virology

, 154:1625 | Cite as

Molecular detection and prevalence of porcine caliciviruses in eastern China from 2008 to 2009

  • Quan Shen
  • Wen Zhang
  • Shixing Yang
  • Yan Chen
  • Huibo Ning
  • Tongling Shan
  • Junfeng Liu
  • Zhibiao Yang
  • Li Cui
  • Jianguo Zhu
  • Xiuguo HuaEmail author
Original Article


Caliciviruses causing diarrhea have been reported in both industrial and developing countries, including China, in recent years. Porcine caliciviruses that are closely related to human sapoviruses (SaVs) and noroviruses (NoVs) have also been detected in swine, which has raised discussion about the animal reservoir and the potential risk for zoonotic transmission to humans. The objective of this work was to determine the frequency and age distribution of SaVs and NoVs in pigs and to characterize the strains prevalent in eastern China. A total of 904 stool samples from pigs of different ages were collected from eastern China from April 2008 to March 2009 and tested for both SaVs and NoVs using reverse transcription-polymerase chain reaction (RT–PCR). Our results indicate that 8 (0.9%) stool samples were positive for SaVs and 2 (0.2%) for NoVs. Phylogenetic analysis of partial sequences of the RNA-dependent RNA polymerase (RdRp) gene indicated that all of the SaV strains belonged to the GIII SaVs, while the two NoV strains belonged to the GII NoV genogroup. The 8 SaV strains were further divided into two clusters, which clustered closely with the Netherlands isolate (AY615804) and the Chinese strain (EU599212), respectively. The two NoV strains shared about 67.3–67.6% nucleotide homology with a human norovirus strain (DQ369797), the only NoV strain from mainland China available in GenBank. Moreover, our results suggest that SaV infections are more frequent in 0-1 month-old pigs than in older ones. In conclusion, the present study provides evidence that PoSaVs and PoNoVs exist in swine in eastern China.


Stool Sample Nucleotide Homology Korean Isolate Sapovirus SaVs Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by Key Project of Shanghai Science and Technology Committee of China under Grant No. 063919121.


  1. 1.
    Barry AF, Alfieri AF, Alfieri AA (2008) High genetic diversity in RdRp gene of Brazilian porcine sapovirus strains. Vet Microbiol 131(1/2):185–191PubMedCrossRefGoogle Scholar
  2. 2.
    Costantini V, Loisy F, Joens L, Le Guyader FS, Saif LJ (2006) Human and animal enteric caliciviruses in oysters from different coastal regions of the United States. Appl Environ Microbiol 72(3):1800–1809PubMedCrossRefGoogle Scholar
  3. 3.
    Fang ZY, Xie HP, LV HX, Zhang Q, Duan ZJ, Steele D, Jiang B, Jiang X (2007) Investigation of human calicivirus (HuCV) diarrhea among infantile and young children in China, 1999–2005. Bing Du Xue Bao 23(1):9–15PubMedGoogle Scholar
  4. 4.
    Farkas T, Zhong WM, Jing Y, Huang PW, Espinosa SM, Martinez N, Morrow AL, Ruiz-Palacios GM, Pickering LK, Jiang X (2004) Genetic diversity among sapoviruses. Arch Virol 149(7):1309–1323PubMedCrossRefGoogle Scholar
  5. 5.
    Geissler K, Schneider K, Platzer G, Truyen B, Kaaden OR, Truyen U (1997) Genetic and antigenic heterogeneity among feline calicivirus isolates from distinct disease manifestations. Virus Res 48(2):193–206PubMedCrossRefGoogle Scholar
  6. 6.
    Guo M, Chang KO, Hardy ME, Zhang Q, Parwani AV, Saif LJ (1999) Molecular characterization of a porcine enteric calicivirus genetically related to Sapporo-like human caliciviruses. J Virol 73:9625–9631PubMedGoogle Scholar
  7. 7.
    Guo M, Evermann JF, Saif LJ (2001) Detection and molecular characterization of cultivable caliciviruses from clinically normal mink and enteric caliciviruses associated with diarrhea in mink. Arch Virol 146:479–493PubMedCrossRefGoogle Scholar
  8. 8.
    Green KY, Ando T, Balayan MS, Berke T, Clarke IN, Estes MK, Matson DO, Nakata S, Neill JD, Studdert MJ, Thiel HJ (2000) Taxonomy of the caliciviruses. J Inf Dis 181(Suppl 2):S322–S330Google Scholar
  9. 9.
    Green KY, Chanock RM, Kapikian AZ (2001) Human caliciviruses. In: Knipe DM, Howley PM et al (eds) Fields virology, 4th edn. Lippinocott Williams &Wilkins, Philadelphia, pp 841–874Google Scholar
  10. 10.
    Jeong C, Park SI, Park SH, Kim HH, Park SJ, Jeong JH, Choy HE, Saif LJ, Kim SK, Kang MI, Hyun BH, Cho KO (2007) Genetic diversity of porcine sapoviruses. Vet Microbiol 122(3/4):246–257PubMedCrossRefGoogle Scholar
  11. 11.
    Jiang X, Huang PW, Zhong WM, Farkas T, Cubitt DW, Matson DO (1999) Design and evaluation of a primer pair that detects both Norwalk-and Sapporo-like caliciviruses by RT–PCR. J Virol Methods 83(1/2):145–154PubMedCrossRefGoogle Scholar
  12. 12.
    Kim HJ, Cho HS, Cho KO, Park NY (2006) Detection and molecular characterization of porcine enteric calicivirus in Korea, genetically related to sapoviruses. J Vet Med B Inft Dis Vet Public Health 53(4):155–159Google Scholar
  13. 13.
    Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5(2):150–163PubMedCrossRefGoogle Scholar
  14. 14.
    L’Homme Y, Sansregret R, Plante-Fortier E, Lamontagne AM, Lacroix G, Ouardani M, Deschamps J, Simard G, Simard C (2009) Genetic diversity of porcine Norovirus and Sapovirus: Canada, 2005–2007. Arch Virol 154(4):581–593PubMedCrossRefGoogle Scholar
  15. 15.
    Martínez MA, Alcalá AC, Carruyo G, Botero L, Liprandi F, Ludert JE (2006) Molecular detection of porcine enteric caliciviruses in Venezuelan farms. Vet Microbiol 116(1–3):77–84PubMedCrossRefGoogle Scholar
  16. 16.
    Martella V, Bányai K, Lorusso E, Bellacicco AL, Decaro N, Mari V, Saif L, Costantini V, De Grazia S, Pezzotti G, Lavazza A, Buonavoglia C (2008) Genetic heterogeneity of porcine enteric caliciviruses identified from diarrhoeic piglets. Virus Genes 36(2):365–373PubMedCrossRefGoogle Scholar
  17. 17.
    Martella V, Lorusso E, Banyai K, Decaro N, Corrente M, Elia G, Cavalli A, Radogna A, Costantini V, Saif LJ, Lavazza A, Di Trani L, Buonavoglia C (2008) Identification of a porcine calicivirus related genetically to human sapoviruses. J Clin Microbiol 46(6):1907–1913PubMedCrossRefGoogle Scholar
  18. 18.
    Matthijnssens J, Ciarlet M, Heiman E, Arijs I, Delbeke T, McDonald SM, Palombo EA, Iturriza-Gómara M, Maes P, Patton JT, Rahman M, Van Ranst M (2008) Full genome-based classification of rotaviruses reveals a common origin between human Wa-like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. J Virol 82(7):3204–3219PubMedCrossRefGoogle Scholar
  19. 19.
    Mauroy A, Scipioni A, Mathijs E, Miry C, Ziant D, Thys C, Thiry E (2008) Noroviruses and sapoviruses in pigs in Belgium. Arch Virol 153(10):1927–1931PubMedCrossRefGoogle Scholar
  20. 20.
    Ohlinger VF, Haas B, Meyers G, Weiland F, Thiel HJ (1990) Identification and characterization of the virus causing rabbit hemorrhagic disease. J Virol 64:3331–3336PubMedGoogle Scholar
  21. 21.
    Reuter G, Bíró H, Szucs G (2007) Enteric caliciviruses in domestic pigs in Hungary. Arch Virol 152(3):611–614PubMedCrossRefGoogle Scholar
  22. 22.
    Scipioni A, Mauroy A, Vinjé J, Thiry E (2008) Animal noroviruses. Vet J 178(1):32–45PubMedCrossRefGoogle Scholar
  23. 23.
    Van der Poel WH, Vinjé J, van der Heide R, Herrera MI, Vivo A, Koopmans MP (2000) Norwalk-like calicivirus genes in farm animals. Emerg Inf Dis 6(1):36–41Google Scholar
  24. 24.
    Wang QH, Costantini V, Saif LJ (2007) Porcine enteric caliciviruses: genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology. Vaccine 25:5453–5466PubMedCrossRefGoogle Scholar
  25. 25.
    Wang QH, Han MG, Cheetham S, Souza M, Funk JA, Saif LJ (2005) Porcine noroviruses related to human noroviruses. Emerg Inf Dis 11(12):1874–1881Google Scholar
  26. 26.
    Wang QH, Souza M, Funk JA, Zhang W, Saif LJ (2006) Prevalence of noroviruses and sapoviruses in swine of various ages determined by reverse transcription-PCR and microwell hybridization assays. J Clin Microbiol 44:2057–2062PubMedCrossRefGoogle Scholar
  27. 27.
    Yu JN, Kim MY, Kim DG, Kim SE, Lee JB, Park SY, Song CS, Shin HC, Seo KH, Choi IS (2008) Prevalence of hepatitis E virus and sapovirus in post-weaning pigs and identification of their genetic diversity. Arch Virol 153(4):739–742PubMedCrossRefGoogle Scholar
  28. 28.
    Zhang W, Shen Q, Hua X, Cui L, Liu J, Yang S (2008) The first Chinese porcine sapovirus strain that contributed to an outbreak of gastroenteritis in piglets. J Virol 82(16):8239–8240PubMedCrossRefGoogle Scholar
  29. 29.
    Zheng DP, Ando T, Fankhauser RL, Beard RS, Glass RI, Monroe SS (2006) Norovirus classification and proposed strain nomenclature. Virology 346(2):312–323PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Quan Shen
    • 1
  • Wen Zhang
    • 2
  • Shixing Yang
    • 1
  • Yan Chen
    • 1
  • Huibo Ning
    • 1
    • 3
  • Tongling Shan
    • 1
  • Junfeng Liu
    • 1
  • Zhibiao Yang
    • 1
  • Li Cui
    • 1
  • Jianguo Zhu
    • 1
  • Xiuguo Hua
    • 1
    Email author
  1. 1.Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and BiologyShanghai JiaoTong UniversityShanghaiPeople’s Republic of China
  2. 2.School of Medical TechnologyJiangsu UniversityZhenjiangPeople’s Republic of China
  3. 3.Shanxi Agricultural UniversityShanxiChina

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