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Archives of Virology

, 154:1685 | Cite as

Phylogenetic analysis of a near-full-length sequence of an erythrovirus genotype 3 strain isolated in Brazil

  • Lilian Walsh Keller
  • Maria Luisa Barbosa
  • Fernando Lucas Melo
  • Lilian M. Pereira
  • Elias David-Neto
  • Luiz E. Lanhez
  • Edison Luiz Durigon
Brief Report

Abstract

Human parvovirus B19 is the only member of the genus Erythrovirus that causes human disease. Recent findings of several strains with considerable sequence divergence from B19 have suggested a new classification for parvovirus genotypes as 1 (B19), 2 (A-6 and LaLi) and 3 (V9). In their overall DNA sequence, the three genotypes differ by ~10%. Here, we report the isolation of a genotype-3-related strain named BR543 during a prospective study conducted in Sao Paulo, Brazil. Analysis of the nearly full-length genome sequence of BR543 indicates that this B19 variant sequence clusters with Gh2768, a strain from Ghana belonging to subtype 3b, and showed mostly synonymous substitutions.

Keywords

BR543 Strain Erythema Infectiosum Good Evolutionary Model Considerable Sequence Divergence Common Human Pathogen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Anderson LJ, Hurwitz ES (1988) Human parvovirus B19 and pregnancy. Clin Perinatol 15(2):273–286 (Review)PubMedGoogle Scholar
  2. 2.
    Anderson MJ, Jones SE, Fisher-Hoch SP, Lewis E, Hall SM, Barlett CRL, Cohen BJ, Mortimer PP, Pereira MS (1983) The human parvovirus, cause of erythema infectiosum. Lancet 1:1378PubMedCrossRefGoogle Scholar
  3. 3.
    Candotti D, Etiz N, Parsyan A, Allain JP (2004) Identification and characterization of persistent human erythrovirus infection in blood donor samples. J Virol 78:12168–12178CrossRefGoogle Scholar
  4. 4.
    Durigon EL, Erdman DD, Gary GW, Pallansch MA, Torok TT, Anderson LJ (1993) Multiple primer pairs for polymerase chain reaction (PCR) amplification of human parvovirus B19 DNA. J Virol Met 44:155–165CrossRefGoogle Scholar
  5. 5.
    Erdman DD, Durigon EL, Wang QY, Anderson LJ (1996) Genetic diversity of human parvovirus B19: sequence analysis of the VP1/VP2 gene from multiple isolates. J Gen Virol 77:2767–2774PubMedCrossRefGoogle Scholar
  6. 6.
    Freitas RB, Melo FL, Oliveira DS, Romano CM, Freitas MRC, Macedo O, Linhares AC, Zanotto PMA, Durigon EL (2008) Molecular characterization of human erythrovirus B19 strains obtained from patients with several clinical presentations in the Amazon region of Brazil. J Clin Virol 43:60–65PubMedCrossRefGoogle Scholar
  7. 7.
    Parsyan A, Szmaragd C, Allain JP, Candotti D (2007) Identification and genetic diversity of two human parvovirus B19 genotype 3 subtypes. J Gen Virol 88:428–431PubMedCrossRefGoogle Scholar
  8. 8.
    Pattison JR, Jones SE, Hodgson J, Davis LR, White JM, Stroud CE, Murtaza L (1981) Parvovirus infections and hypoplastic crisis sickle-cell anaemia. Lancet 1:604–665Google Scholar
  9. 9.
    Posada D, Crandall KA (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818PubMedCrossRefGoogle Scholar
  10. 10.
    Reid DM, Reid TMS, Brown T, Rennie JAN, Eastmond CJ (1985) Human parvovirus associated arthritis: a clinical and laboratory description. Lancet 2:422–425CrossRefGoogle Scholar
  11. 11.
    Sanabani S, Kleine WM, Pereira J, Sabino EC (2006) Sequence variability of human erythrovirus present in bone marrow of Brazilian patients with various parvovirus B19-related hematological symptoms. J Clin Microbiol 44:604–606PubMedCrossRefGoogle Scholar
  12. 12.
    Servant A, Laperche S, Lallemand F, Marinho V, De Saint Maur G, Meritet JF, Garbarg-Chenon A (2002) Genetic diversity within human erythrovirus: identification of three genotypes. J Virol 76:9124–9134PubMedCrossRefGoogle Scholar
  13. 13.
    Shade RO, Blumdell MC, Cotmore SF, Tattersall P, Astell CR (1986) Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of child during aplastic crisis. J Virol 58:921–936PubMedGoogle Scholar
  14. 14.
    Young NS (1996) Parvovirus. In: Fields BN, Knipe DM, Howley PM (eds) Fields virology. Lippincott-Raven, Philadelphia, p 2190Google Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Lilian Walsh Keller
    • 1
  • Maria Luisa Barbosa
    • 2
  • Fernando Lucas Melo
    • 1
  • Lilian M. Pereira
    • 3
  • Elias David-Neto
    • 3
  • Luiz E. Lanhez
    • 3
  • Edison Luiz Durigon
    • 1
  1. 1.Institute of Biomedical Science, Department of MicrobiologyUniversity of Sao PauloSao PauloBrazil
  2. 2.Institute Adolfo LutzSao PauloBrazil
  3. 3.Renal Transplantation Unit, Hospital of ClinicsUniversity of Sao PauloSao PauloBrazil

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