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Archives of Virology

, Volume 154, Issue 8, pp 1343–1347 | Cite as

Molecular characterization of a closterovirus from carrot and its identification as a German isolate of Carrot yellow leaf virus

  • Wulf Menzel
  • Reinhard Goetz
  • Dietrich E. Lesemann
  • H. Josef Vetten
Brief Report

Abstract

A high-molecular-weight dsRNA (~15 kbp) was isolated from chlorotic leaves of a carrot plant and used for determining the entire nucleotide sequence of a closterovirus. The complete genome of this carrot closterovirus (CCV) was 16.4 kb in length and contained ten open reading frames (ORFs). The genome organization of CCV resembled that of beet yellow stunt virus, but ORF2 and ORF3 were in a reversed order. Based on Hsp70h sequences, CCV is most closely related to carnation necrotic fleck virus and mint virus 1, two viruses of the genus Closterovirus (family Closteroviridae). The major coat protein gene of CCV was expressed in Escherichia coli for raising an antiserum. This permitted routine detection of CYLV by DAS-ELISA and immunoelectron microscopy and was used for demonstrating the bipolar nature of the CCV virion. Moreover, the antiserum gave a Western blot reaction with a reference sample of a Carrot yellow leaf virus (CYLV) isolate from the Netherlands, suggesting that CCV is a German isolate of CYLV.

Keywords

Coat Protein Citrus Tristeza Virus Carrot Plant Beet Yellow Virus German Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors thank R. van der Vlugt (Wageningen, The Netherlands) for providing a dried leaf sample of the Dutch CYLV isolate. This work was part of a study supported by the German Ministry of Food, Agriculture and Consumer Protection under project no. 02OE253.

Supplementary material

705_2009_428_MOESM1_ESM.pdf (36 kb)
Supplementary Figure (pdf 37kb)

References

  1. 1.
    Agranovsky AA, Koonin EV, Boyko VP, Maiss E, Frötschl R, Lunina NA, Atabekov JG (1994) Beet yellows closterovirus: complete genome structure and identification of a leader papain-like thiol protease. Virology 198:311–324PubMedCrossRefGoogle Scholar
  2. 2.
    Agranovsky AA, Lesemann D-E, Maiss E, Hull R, Atabekov JG (1995) “Rattlesnake” structure of a filamentous plant RNA virus built of two capsid proteins. Proc Natl Acad Sci USA 92:2470–2473PubMedCrossRefGoogle Scholar
  3. 3.
    Alzhanova DV, Prokhnevsky AI, Peremyslov VV, Dolja VV (2007) Virion tails of Beet yellows virus: coordinated assembly by three structural proteins. Virology 359:220–226PubMedCrossRefGoogle Scholar
  4. 4.
    Bem F, Murant AF (1979) Transmission and differentiation of six viruses infecting hogweed (Heracleum sphondylium) in Scotland. Ann App Biol 92:237–242CrossRefGoogle Scholar
  5. 5.
    Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34:475–483PubMedCrossRefGoogle Scholar
  6. 6.
    Dolja VV, Kreuze JF, Valkonen JPT (2006) Comparative and functional genomics of closteroviruses. Virus Res 117:38–51PubMedCrossRefGoogle Scholar
  7. 7.
    Febres VJ, Ashoulin L, Mawassi M, Frank A, Bar-Joseph M, Manjunath KL, Lee RF, Niblett CL (1996) The p27 protein is present at one end of citrus tristeza virus particles. Phytopathology 86:1331–1335Google Scholar
  8. 8.
    Froussard P (1992) A random-PCR method (rPCR) to construct whole cDNA library from low amounts of RNA. Nucleic Acids Res 11:2900CrossRefGoogle Scholar
  9. 9.
    Koenig R, Pleijl CWA, Loss S, Burgermeister W, Aust H, Schiemann J (2004) Molecular characterisation of potexviruses isolated from three different genera in the family Cactaceae. Arch Virol 149:903–914PubMedCrossRefGoogle Scholar
  10. 10.
    Koonin EV (1991) The phylogeny of RNA dependent RNA polymerases of positive-strand RNA viruses. J Gen Virol 72:2197–2206PubMedCrossRefGoogle Scholar
  11. 11.
    Martelli GP, Agranovsky AA, Bar-Joseph M, Boscia D, Candresse T, Coutts RHA, Dolja VV, Falk BW, Gonsalves D, Hu JS, Jelkmann W, Karasev AV, Minafra A, Namba S, Vetten HJ, Wisler CG, Yoshikawa N (2005) Closteroviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy. Academic Press, London, pp 1077–1087Google Scholar
  12. 12.
    Milne RG, Lesemann D-E (1984) Immunosorbent electron microscopy in plant virus studies. Methods Virol 8:5–101Google Scholar
  13. 13.
    Schultz J, Milpetz F, Bork P, Ponting CP (1998) SMART, a simple modular architecture research tool: identification of signaling domains. Proc Natl Acad Sci USA 95:5857–5864PubMedCrossRefGoogle Scholar
  14. 14.
    Tian T, Rubio L, Yeh HH, Crawford B, Falk BW (1999) Lettuce infectious yellows virus: in vitro acquisition analysis using partially purified virions and the whitefly, Bemisia tabaci. J Gen Virol 80:1111–1117PubMedGoogle Scholar
  15. 15.
    Tzanetakis IE, Martin RR (2007) Strawberry chlorotic fleck: identification and characterization of a novel closterovirus associated with the disease. Virus Res 124:88–94PubMedCrossRefGoogle Scholar
  16. 16.
    Valverde RA, Dodds JA, Heick JA (1985) Double-stranded nucleic acid from plants infected with viruses having elongated particles and undivided genomes. Phytopathology 75:1357–1367CrossRefGoogle Scholar
  17. 17.
    van Dijk P, Bos L (1985) Viral dieback of carrot and other Umbelliferae caused by the Anthriscus strain of parsnip yellow fleck virus, and its distinction from carrot motley dwarf. Neth J Plant Pathol 91:169–187CrossRefGoogle Scholar
  18. 18.
    van Dijk P, Bos L (1989) Survey and biological differentiation of viruses of wild and cultivated Umbelliferae in the Netherlands. Neth J Plant Pathol 95(Suppl. 2):1–34CrossRefGoogle Scholar
  19. 19.
    van Dijk P, Bos L (1996) Carrot yellow leaf virus. In: Brunt AA, Crabtree K, Dallwitz MJ, Gibbs AJ, Watson L (eds) Viruses of Plants. Descriptions and Lists from the VIDE Database. CAB International, pp 325–326Google Scholar
  20. 20.
    Vetten HJ, Lesemann D-E, Maiss E (1992) Serotype A and B strains of bean common mosaic virus are two distinct potyviruses. Arch Virol Suppl 5:415–431PubMedGoogle Scholar
  21. 21.
    Yamashita S, Ohki S, Doi Y, Yora K (1976) Identification of two viruses associated with the carrot yellow leaf syndrome. Ann Phytopathol Soc Jpn 42:382–383 (in Japanese)Google Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Wulf Menzel
    • 1
    • 2
  • Reinhard Goetz
    • 1
  • Dietrich E. Lesemann
    • 1
  • H. Josef Vetten
    • 1
  1. 1.Federal Research Centre for Cultivated Plants, Institute of Epidemiology and Pathogen DiagnosticsJulius Kuehn-Institute (JKI)BraunschweigGermany
  2. 2.Institute of Plant Diseases and Plant ProtectionUniversity of HannoverHannoverGermany

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