Archives of Virology

, Volume 154, Issue 8, pp 1343–1347 | Cite as

Molecular characterization of a closterovirus from carrot and its identification as a German isolate of Carrot yellow leaf virus

  • Wulf Menzel
  • Reinhard Goetz
  • Dietrich E. Lesemann
  • H. Josef Vetten
Brief Report


A high-molecular-weight dsRNA (~15 kbp) was isolated from chlorotic leaves of a carrot plant and used for determining the entire nucleotide sequence of a closterovirus. The complete genome of this carrot closterovirus (CCV) was 16.4 kb in length and contained ten open reading frames (ORFs). The genome organization of CCV resembled that of beet yellow stunt virus, but ORF2 and ORF3 were in a reversed order. Based on Hsp70h sequences, CCV is most closely related to carnation necrotic fleck virus and mint virus 1, two viruses of the genus Closterovirus (family Closteroviridae). The major coat protein gene of CCV was expressed in Escherichia coli for raising an antiserum. This permitted routine detection of CYLV by DAS-ELISA and immunoelectron microscopy and was used for demonstrating the bipolar nature of the CCV virion. Moreover, the antiserum gave a Western blot reaction with a reference sample of a Carrot yellow leaf virus (CYLV) isolate from the Netherlands, suggesting that CCV is a German isolate of CYLV.


Coat Protein Citrus Tristeza Virus Carrot Plant Beet Yellow Virus German Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors thank R. van der Vlugt (Wageningen, The Netherlands) for providing a dried leaf sample of the Dutch CYLV isolate. This work was part of a study supported by the German Ministry of Food, Agriculture and Consumer Protection under project no. 02OE253.

Supplementary material

705_2009_428_MOESM1_ESM.pdf (36 kb)
Supplementary Figure (pdf 37kb)


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Wulf Menzel
    • 1
    • 2
  • Reinhard Goetz
    • 1
  • Dietrich E. Lesemann
    • 1
  • H. Josef Vetten
    • 1
  1. 1.Federal Research Centre for Cultivated Plants, Institute of Epidemiology and Pathogen DiagnosticsJulius Kuehn-Institute (JKI)BraunschweigGermany
  2. 2.Institute of Plant Diseases and Plant ProtectionUniversity of HannoverHannoverGermany

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