Field assessment of an H5N1 inactivated vaccine in chickens and ducks in Lao PDR
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Despite the extensive use of poultry vaccines to control the spread of H5N1 influenza in poultry, H5N1 outbreaks continue to occur in domestic birds. Our objective was to determine the duration of the neutralizing antibody response under field conditions after vaccination with a laboratory-tested inactivated reverse genetics-derived H5N3 vaccine. H5N3 hemagglutination inhibition (HI) and virus neutralization (VN) antibodies were observed 40 weeks after vaccination of chickens with two doses and vaccination of ducks with one dose. Cross-clade antibodies to an H5N1 virus (A/chicken/Laos/A0464/07) antigenically distinct from the vaccine strain were detected in ducks after a single vaccination and were sustained for 28 weeks (for 40 weeks when a boost vaccination was given). Our results indicate that this inactivated H5N3 vaccine can produce long-lasting antibodies to homologous and heterologous viruses under field conditions.
This work was supported by contract HHSN266200700005C from the National Institute of Allergy and Infectious Diseases and by the American Lebanese Syrian Associated Charities (ALSAC). Sincere thanks to Dr. Bounkhouang Khambounheuang, Director General of the Lao PDR Department of Livestock and Fisheries, for his support, and to the Vaccine Production Centre of that Department for the use of its laboratory and animal husbandry facilities. We thank Scott Krauss for technical assistance and Sharon Naron for editorial assistance.
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