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Archives of Virology

, Volume 154, Issue 4, pp 581–593 | Cite as

Genetic diversity of porcine Norovirus and Sapovirus: Canada, 2005–2007

  • Yvan L’Homme
  • Raphaël Sansregret
  • Étienne Plante-Fortier
  • Anne-Marie Lamontagne
  • Geneviève Lacroix
  • Mourad Ouardani
  • Jonathan Deschamps
  • Geneviève Simard
  • Carole Simard
Original Article

Abstract

Noroviruses and sapoviruses are members of the family Caliciviridae and emerging enteric pathogens of humans and animals. Since their discovery and characterization in swine, relatively few strains have been described in detail. In order to investigate their genetic diversity, a total of 266 fecal samples collected in the province of Quebec, Canada, between 2005 and 2007 were screened for the presence of caliciviruses by RT-PCR using broadly reactive primers. Genetically heterogeneous caliciviruses were detected on the majority of farms. Typical noroviruses related to known swine genotypes were present on 20% of the farms. Sapoviruses were detected on 75% of the farms and were the most heterogeneous group. Further characterization of selected strains in their 3′ end parts was carried out for their classification and unveiled possibly new clusters of sapoviruses. No human-like noroviruses or sapoviruses were detected in the present study.

Keywords

Sapovirus Human NoVs Porcine SaVs Swine Strain Porcine NoVs 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by the Science Division of the Canadian Food Inspection Agency (CFIA). Special thanks to Denis L’Homme for helpful discussions on phylogenies.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Yvan L’Homme
    • 1
  • Raphaël Sansregret
    • 1
  • Étienne Plante-Fortier
    • 1
  • Anne-Marie Lamontagne
    • 1
  • Geneviève Lacroix
    • 1
  • Mourad Ouardani
    • 1
  • Jonathan Deschamps
    • 1
  • Geneviève Simard
    • 1
  • Carole Simard
    • 1
  1. 1.St-Hyacinthe LaboratoryCanadian Food Inspection AgencySt-HyacintheCanada

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