Archives of Virology

, Volume 154, Issue 5, pp 755–763

A longitudinal cohort study in calves evaluated for rotavirus infections from 1 to 12 months of age by sequential serological assays

  • Dianjun Cao
  • Blessing Igboeli
  • Lijuan Yuan
  • Albert Z. Kapikian
  • Jess L. Ayers
  • Francis R. Abinanti
  • Yasutaka Hoshino
Original Article

Abstract

Using an immunocytochemical staining assay involving six different recombinant baculoviruses with each expressing one of the major bovine rotavirus VP7 (G6, G8 and G10) and VP4 (P6[1], P7[5] and P8[11]) serotypes, we analyzed IgG antibody responses to individual proteins in archival serum samples collected from 31 calves monthly from 1 to 12 months of age during 1974–1975 in Higley, Arizona. Seroresponses to VP7 and VP4, as determined by a fourfold or greater antibody response, were not always elicited concurrently following infection: in some calves, (1) seroresponses to VP7 were detected earlier than to VP4 or vice versa; and (2) a subsequent second seroresponse was detected for VP7 or VP4 only. In addition, a second infection was more likely to be caused by different G and/or P types. Analyses of serum samples showed that the most frequent G–P combination was G8P6[1], followed by G8P7[5], G8P8[11] and G6P6[1].

Copyright information

© US Government 2009

Authors and Affiliations

  • Dianjun Cao
    • 1
  • Blessing Igboeli
    • 1
    • 4
  • Lijuan Yuan
    • 2
  • Albert Z. Kapikian
    • 1
  • Jess L. Ayers
    • 3
    • 5
  • Francis R. Abinanti
    • 3
  • Yasutaka Hoshino
    • 1
  1. 1.Epidemiology Section, Laboratory of Infectious DiseasesNational Institute of Allergy and Infectious Diseases, National Institutes of HealthBethesdaUSA
  2. 2.Virology and Immunology Department of Biomedical Sciences and PathobiologyVirginia-Maryland Regional College of Veterinary MedicineBlacksburgUSA
  3. 3.Department of Veterinary ScienceUniversity of ArizonaTucsonUSA
  4. 4.Case Western Reserve University School of MedicineClevelandUSA
  5. 5.Ayers Animal ClinicSanta MariaUSA

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