Archives of Virology

, Volume 154, Issue 3, pp 409–419 | Cite as

Multiorgan distribution of human influenza A virus strains observed in a mouse model

  • T. Fislová
  • M. Gocník
  • T. Sládková
  • V. Ďurmanová
  • J. Rajčáni
  • E. Varečková
  • V. Mucha
  • F. KostolanskýEmail author
Original Article


Multiorgan spread and pathogenesis of influenza infection with three human influenza A viruses was studied in mice. Mouse-adapted viruses A/Dunedin/4/73(H3N2), A/Mississippi/1/85(H3N2), and A/PR/8/34(H1N1) differed considerably in virulence (p.f.u./LD50): 79,000 p.f.u. for Dunedin, 5,000 p.f.u. for Mississippi, and 65 p.f.u. for PR/8, which qualified Dunedin as low virulent, Mississippi as intermediate, and PR/8 as highly virulent. All three viruses were detected in lungs, heart, and thymus by cultivation and RT-PCR. Moreover, vRNA of all viruses was found in liver and spleen, of Dunedin and PR/8 also in kidneys and that of Dunedin and Mississippi in blood. Only vRNA of Dunedin was demonstrated in brain. Lung damage accompanied by histopathological changes and thymus reduction were most extensive after infection with the highly virulent virus PR/8. We assume that the ability to spread to multiple organs may be a more common property of influenza viruses in mammalian hosts than previously believed.


Influenza Influenza Virus Avian Influenza MDCK Cell Influenza Infection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors are thankful to Jana Mesárošová and Matilda Némethová for their excellent technical assistance and Katarína Polčicová for reading the manuscript. This work was supported by the Scientific Grant Agency of the Ministry of Education of the Slovak Republic and the Slovak Academy of Sciences, grant nos. 2/7065/27, 2/6077/27, 2/0154/09 and by the Slovak Research and Development Agencyn under contract no. APVV-51-021605.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • T. Fislová
    • 1
  • M. Gocník
    • 1
  • T. Sládková
    • 1
  • V. Ďurmanová
    • 1
  • J. Rajčáni
    • 1
  • E. Varečková
    • 1
  • V. Mucha
    • 1
  • F. Kostolanský
    • 1
    Email author
  1. 1.Institute of Virology, Slovak Academy of SciencesBratislavaSlovak Republic

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