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Archives of Virology

, 154:255 | Cite as

Large accumulations of maize streak virus in the filter chamber and midgut cells of the leafhopper vector Cicadulina mbila

  • El-Desouky Ammar
  • Daniel Gargani
  • Jean M. Lett
  • Michel Peterschmitt
Original Article

Abstract

Maize streak virus (MSV, Mastrevirus, Geminiviridae) is persistently transmitted by Cicadulina mbila, apparently without propagation in its leafhopper vector. MSV was shown earlier by quantitative PCR to accumulate in the alimentary canal of C. mbila. We examined the alimentary canals of C. mbila leafhoppers that acquired MSV from diseased plants for various acquisition access periods (AAP) by immunofluorescence confocal laser scanning microscopy (iCLSM) and by immunogold labelling transmission electron microscopy (iTEM). Following a 7-day AAP and a 7-day inoculation period (IP) on healthy seedlings, MSV was detected by iCLSM mainly in the filter chamber and anterior midgut. Using iTEM, large accumulations of MSV particles, usually enclosed in membranous vesicles, were detected only in cells of the midgut, inside and outside the filter chamber, following 14- or 30-day AAPs, and also following 7-day AAP and 7-day IP on healthy plants. No virus was detected in the control non-vector species C. chinaï. Coated pits or vesicles, typical of clathrin-mediated endocytosis, were not observed. We discuss an alternative endocytosis pathway and suggest that the MSV accumulations are stored in endosomes in the midgut epithelial cells.

Keywords

Tomato Yellow Leaf Curl Virus Alimentary Canal Malpighian Tubule Maize Streak Virus Midgut Epithelial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We are thankful to Martine Granier who took care of the leafhopper rearing. We also thank CIRAD for a research and travel grant that enabled E.-D. Ammar to work on this project at CIRAD, Montpellier, France.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • El-Desouky Ammar
    • 1
    • 2
  • Daniel Gargani
    • 1
  • Jean M. Lett
    • 1
    • 3
  • Michel Peterschmitt
    • 1
  1. 1.CIRAD, UMR BGPIMontpellier Cedex 5France
  2. 2.Department of EntomologyOhio State University, OARDCWoosterUSA
  3. 3.CIRAD, UMR PVBMTPôle de Protection des PlantesSaint Pierre, Ile de La RéunionFrance

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