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Archives of Virology

, Volume 154, Issue 2, pp 353–359 | Cite as

Genetically distinct strains of Cassava brown streak virus in the Lake Victoria basin and the Indian Ocean coastal area of East Africa

  • D. R. Mbanzibwa
  • Y. P. Tian
  • A. K. Tugume
  • S. B. Mukasa
  • F. Tairo
  • S. Kyamanywa
  • A. Kullaya
  • Jari P. T. Valkonen
Brief Report

Abstract

Six isolates of Cassava brown streak virus (CBSV, genus Ipomovirus; Potyviridae) from the Lake Victoria basin in Uganda and Tanzania were characterized. Virus particles were 650 nm long. The complete coat protein (CP)-encoding sequences (1,101 nucleotides, nt) were 90.7–99.5 and 93.7–99.5% identical at the nt and amino acid (aa) levels, respectively. The 3′ untranslated region was 225, 226 or 227 nt long. These eight isolates were only 75.8–77.5% (nt) and 87.0–89.9% (aa) identical when compared to the partial CP sequences (714 nt) of six CBSV isolates characterized previously from the costal lowlands of Tanzania and Mozambique. Hence, two genetically different and geographically separated populations of CSBV exist in East Africa.

Keywords

Coat Protein Reverse Transcription Polymerase Chain Reaction Cetyl Trimethyl Ammonium Bromide Cassava Plant Coastal Lowland 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Anu Kallinen, Johanna Aura, Marja Calonius, Sascha Kirchner and Peter Wasswa for technical assistance. This study was part of the project “Towards Sustainable Cassava and Sweet Potato Production in Eastern Africa” under the East African Regional Network for Biotechnology, Biosafety and Biotechnology Policy Development programme (BIO-EARN). Funding from Sida, Sweden, and the assistance of the Stockholm Environment Institute (SEI) are gratefully acknowledged.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • D. R. Mbanzibwa
    • 1
    • 2
    • 3
  • Y. P. Tian
    • 1
  • A. K. Tugume
    • 1
    • 4
  • S. B. Mukasa
    • 2
  • F. Tairo
    • 3
  • S. Kyamanywa
    • 2
  • A. Kullaya
    • 3
  • Jari P. T. Valkonen
    • 1
  1. 1.Department of Applied BiologyUniversity of HelsinkiHelsinkiFinland
  2. 2.Department of Crop ScienceMakerere UniversityKampalaUganda
  3. 3.Mikocheni Agricultural Research InstituteDar es SalaamTanzania
  4. 4.Department of BotanyMakerere UniversityKampalaUganda

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