Complete nucleotide sequence of chili leaf curl virus and its associated satellites naturally infecting potato in Pakistan
A severe leaf curl disease was observed in 2005 on potato (Solanum tuberosum L.) crops in Lahore, Punjab province, Pakistan. Affected plants were severely stunted with apical leaf curl and crinkled leaves, symptoms typical of virus infection. Potato is an important food crop grown both in the plains and hilly areas of Pakistan. Viral diseases are important constraints on this crop and are usually managed by manipulation of planting dates and use of virus-free seed potato. The crop is affected by several RNA viruses, but no DNA virus infecting potato has been reported so far from Pakistan.
Geminiviruses are single-stranded DNA viruses that depend on insect vectors for transmission . Begomoviruses are whitefly-transmitted geminiviruses that have emerged as a major constraint of food and fiber crops throughout the world but are more common in warmer climates . The majority of begomoviruses are bipartite, but recently, numerous monopartite begomoviruses have been identified that...
KeywordsNucleotide Sequence Identity Monopartite Begomoviruses Virion Sense High Nucleotide Sequence Identity Curly Shoot
M. Mubin is supported by a PhD fellowship from the Higher Education Commission (HEC), Government of Pakistan. R. W. Briddon is supported by the HEC under the “Foreign Faculty Hiring Program”. This research has been supported by projects from the Ministry of Science and Technology (MoST), Government of Pakistan, and the International Center for Genetic Engineering and Biotechnology (ICGEB).
- 7.Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
- 9.Hussain M, Iram S, Mansoor S, Briddon RW (2008) A single species of betasatellite is prevalent in chili across north central Pakistan and shows phylogeographic segregation. J Phytopathol (in press)Google Scholar
- 15.Stanley J, Bisaro DM, Briddon RW, Brown JK, Fauquet CM, Harrison BD, Rybicki EP, Stenger DC (2005) Geminiviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus Taxonomy VIIIth Report of the ICTV. Elsevier/Academic Press, London, pp 301–326Google Scholar