Archives of Virology

, Volume 154, Issue 2, pp 365–368 | Cite as

Complete nucleotide sequence of chili leaf curl virus and its associated satellites naturally infecting potato in Pakistan

  • M. Mubin
  • R. W. Briddon
  • S. Mansoor
Annotated Sequence Record

A severe leaf curl disease was observed in 2005 on potato (Solanum tuberosum L.) crops in Lahore, Punjab province, Pakistan. Affected plants were severely stunted with apical leaf curl and crinkled leaves, symptoms typical of virus infection. Potato is an important food crop grown both in the plains and hilly areas of Pakistan. Viral diseases are important constraints on this crop and are usually managed by manipulation of planting dates and use of virus-free seed potato. The crop is affected by several RNA viruses, but no DNA virus infecting potato has been reported so far from Pakistan.

Geminiviruses are single-stranded DNA viruses that depend on insect vectors for transmission [15]. Begomoviruses are whitefly-transmitted geminiviruses that have emerged as a major constraint of food and fiber crops throughout the world but are more common in warmer climates [11]. The majority of begomoviruses are bipartite, but recently, numerous monopartite begomoviruses have been identified that...


Nucleotide Sequence Identity Monopartite Begomoviruses Virion Sense High Nucleotide Sequence Identity Curly Shoot 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



M. Mubin is supported by a PhD fellowship from the Higher Education Commission (HEC), Government of Pakistan. R. W. Briddon is supported by the HEC under the “Foreign Faculty Hiring Program”. This research has been supported by projects from the Ministry of Science and Technology (MoST), Government of Pakistan, and the International Center for Genetic Engineering and Biotechnology (ICGEB).


  1. 1.
    Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X, Fauquet CM (2008) Recommendations for the classification and nomenclature of the DNA-β satellites of begomoviruses. Arch Virol 153:763–781PubMedCrossRefGoogle Scholar
  2. 2.
    Briddon RW, Bull SE, Amin I, Mansoor S, Bedford ID, Rishi N, Siwatch SS, Zafar MY, Abdel-Salam AM, Markham PG (2004) Diversity of DNA 1; a satellite-like molecule associated with monopartite begomovirus-DNA β complexes. Virology 324:462–474PubMedCrossRefGoogle Scholar
  3. 3.
    Briddon RW, Bull SE, Mansoor S, Amin I, Markham PG (2002) Universal primers for the PCR-mediated amplification of DNA β; a molecule associated with some monopartite begomoviruses. Mol Biotechnol 20:315–318PubMedCrossRefGoogle Scholar
  4. 4.
    Briddon RW, Bull SE, Amin I, Idris AM, Mansoor S, Bedford ID, Dhawan P, Rishi N, Siwatch SS, Abdel-Salam AM, Brown JK, Zafar Y, Markham PG (2003) Diversity of DNA β: a satellite molecule associated with some monopartite begomoviruses. Virology 312:106–121PubMedCrossRefGoogle Scholar
  5. 5.
    Briddon RW, Mansoor S, Bedford ID, Pinner MS, Saunders K, Stanley J, Zafar Y, Malik KA, Markham PG (2001) Identification of DNA components required for induction of cotton leaf curl disease. Virology 285:234–243PubMedCrossRefGoogle Scholar
  6. 6.
    Bull SE, Briddon RW, Markham PG (2003) Universal primers for the PCR-mediated amplification of DNA 1: a satellite-like molecule associated with begomovirus-DNA β complexes. Mol Biotechnol 23:83–86PubMedCrossRefGoogle Scholar
  7. 7.
    Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
  8. 8.
    Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain demarcation and nomenclature. Arch Virol 153:783–821PubMedCrossRefGoogle Scholar
  9. 9.
    Hussain M, Iram S, Mansoor S, Briddon RW (2008) A single species of betasatellite is prevalent in chili across north central Pakistan and shows phylogeographic segregation. J Phytopathol (in press)Google Scholar
  10. 10.
    Mansoor S, Khan SH, Bashir A, Saeed M, Zafar Y, Malik KA, Briddon RW, Stanley J, Markham PG (1999) Identification of a novel circular single-stranded DNA associated with cotton leaf curl disease in Pakistan. Virology 259:190–199PubMedCrossRefGoogle Scholar
  11. 11.
    Mansoor S, Briddon RW, Zafar Y, Stanley J (2003) Geminivirus disease complexes: an emerging threat. Trends Plant Sci 8:128–134PubMedCrossRefGoogle Scholar
  12. 12.
    Page RDM (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358PubMedGoogle Scholar
  13. 13.
    Shahid MS, Mansoor S, Briddon RW (2007) Complete nucleotide sequences of cotton leaf curl Rajasthan virus and its associated DNA β molecule infecting tomato. Arch Virol 152:2131–2134PubMedCrossRefGoogle Scholar
  14. 14.
    Shih SL, Tsai JH, Green SK, Khalid S, Ahmad I, Rezaian MA, Smith J (2003) Molecular characterization of tomato and chili leaf curl begomoviruses from Pakistan. Plant Dis 87:200CrossRefGoogle Scholar
  15. 15.
    Stanley J, Bisaro DM, Briddon RW, Brown JK, Fauquet CM, Harrison BD, Rybicki EP, Stenger DC (2005) Geminiviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus Taxonomy VIIIth Report of the ICTV. Elsevier/Academic Press, London, pp 301–326Google Scholar
  16. 16.
    Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal X windows interface; flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 25:4876–4882PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Plant Biotechnology DivisionNational Institute for Biotechnology and Genetic Engineering (NIBGE)FaisalabadPakistan

Personalised recommendations