Complete genome sequence of Nariva virus, a rodent paramyxovirus
- 197 Downloads
Nariva virus (NarPV) was isolated from forest rodents (Zygodontomys b. brevicauda) in eastern Trinidad in the early 1960s. Initial classification within the family Paramyxoviridae was based mainly on morphological observations including the structure of nucleocapsids and virion surface projections. Here, we report the characterization of the complete genome sequence of NarPV. The genome is 15,276 nucleotides in length, conforming to the rule-of-six, and has a genome organization typical of most members of the family, with six transcriptional units in the order 3′-N–P-M-F–H-L-5′. The gene junctions contain highly conserved gene start and stop signals and a tri-nucleotide intergenic sequence present in most members of the subfamily Paramyxovirinae. Sequence comparison studies indicate that NarPV is most closely related to Mossman virus, which was isolated from wild rats (Rattus leucopus) in Queensland, Australia, in 1970. This study confirmed the classification of NarPV as a member of the subfamily Paramyxovirinae and established the close genome organization and sequence relationship between the two rodent paramyxoviruses isolated almost a decade apart and from two locations separated by more than 15,000 km.
KeywordsNewcastle Disease Virus Measle Virus Editing Site Canine Distemper Virus cDNA Subtraction
We thank Dr. N. Karabatsos for supplying the Nariva virus stock, Kaylene Selleck and Eric Hansson for technical assistance, Tony Pye for providing the automated sequencing service, and Drs. Glenn Marsh and Jackie Pallister for critical review of the manuscript.
- 7.Jack PJM, Boyle DB, Eaton BT, Wang LF (2005) The complete genome sequence of J-virus reveals a unique genome structure in the family Paramyxoviridae. J Virol (in press)Google Scholar
- 10.Lamb RA, Collins PL, Kolakofsky D, Melero JA, Nagai Y, Oldstone MBA, Pringle CR, Rima BK (2005) Family paramyxoviridae. In: Fauquet CM, Mayo J, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy: 8th report of the International Committee on Taxonomy of Viruses. Elsevier, San Diego, pp 655–668Google Scholar
- 11.Lamb RA, Parks GD (2007) Paramyxoviridae: the viruses and their replication. In: Knipe DM, Griffin DE, Lamb RA, Straus SE, Howley PM, Martin MA, Roizman B (eds) Fields virology. Lippincott, Philadelphia, pp 1449–1496Google Scholar
- 22.Tillett D, Burns BP, Neilan BA (2000) Optimized rapid amplification of cDNA ends (RACE) for mapping bacterial mRNA transcripts. Biotechniques 28:448, 450, 443–452, 456Google Scholar
- 24.Wang L-F, Eaton BT (2001) Emerging paramyxoviruses. Infect Dis Rev 3:52–60Google Scholar