Archives of Virology

, Volume 154, Issue 1, pp 121–125 | Cite as

Simultaneous detection of human enterovirus 71 and coxsackievirus A16 in clinical specimens by multiplex real-time PCR with an internal amplification control

  • Xing-Long Xiao
  • Ya-Qing He
  • Yi-Gang Yu
  • Hong Yang
  • Gu Chen
  • Hui-Fang Li
  • Jing-Wei Zhang
  • Dong-Mei Liu
  • Xiao-Feng Li
  • Xiao-Quan Yang
  • Hui Wu
Brief Report

Abstract

The recent and continuing HFMD outbreak caused by EV71 in several provinces of China since March 2008 has affected thousands of children and resulted in nearly 50 deaths. In this study, a sensitive and specific multiplex real-time RT-PCR assay has been developed for the rapid detection of EV71 and CV-A16. By using an internal amplification control, the real-time assay achieves detection of samples containing inhibitors and avoids false negatives. It should prove useful for clinical diagnosis of EV71 or CV-A16 infections.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Xing-Long Xiao
    • 1
    • 2
  • Ya-Qing He
    • 3
  • Yi-Gang Yu
    • 1
  • Hong Yang
    • 3
  • Gu Chen
    • 1
  • Hui-Fang Li
    • 2
  • Jing-Wei Zhang
    • 2
  • Dong-Mei Liu
    • 1
  • Xiao-Feng Li
    • 1
  • Xiao-Quan Yang
    • 1
  • Hui Wu
    • 1
  1. 1.Department of Food, Institution of Food safety, College of Light Industry and FoodSouth China University of TechnologyGuangzhouChina
  2. 2.Shenzhen Taitai Genomics, Inc.ShenzhenChina
  3. 3.Shenzhen Center for Disease Control and PreventionShenzhenChina

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