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Archives of Virology

, Volume 153, Issue 11, pp 2149–2155 | Cite as

Agroinfection of cloned Sri Lankan cassava mosaic virus DNA to Arabidopsis thaliana, Nicotiana tabacum and cassava

  • Dheeraj Mittal
  • Basanta Kumar Borah
  • Indranil DasguptaEmail author
Brief Report

Abstract

Sri Lankan cassava mosaic virus (SLCMV) is a bipartite begomovirus infecting cassava in India and Sri Lanka. We have used Agrobacterium-mediated inoculation (agroinoculation) of cloned SLCMV DNA to inoculate additional hosts, Nicotiana tabacum and Arabidopsis. Although SLCMV infection in these hosts caused stunting, leaf deformation and developmental abnormalities, accumulation levels of viral DNA in the infected plants suggested that this virus was poorly adapted to them. In the natural host, cassava, agroinoculation produced infection at a low frequency. The monopartite nature of SLCMV, reported earlier in N. benthamiana, was maintained in the new hosts as well as in cassava.

Keywords

Benthamiana Plant Symptomatic Plant Cassava Mosaic Disease Nucleotide Residue Biolistic Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

B. K. B. is thankful for the award of a Research Fellowship by the Council of Scientific and Industrial Research, New Delhi.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Dheeraj Mittal
    • 1
  • Basanta Kumar Borah
    • 1
  • Indranil Dasgupta
    • 1
    Email author
  1. 1.Department of Plant Molecular BiologyUniversity of Delhi South CampusNew DelhiIndia

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