Archives of Virology

, Volume 153, Issue 11, pp 2149–2155 | Cite as

Agroinfection of cloned Sri Lankan cassava mosaic virus DNA to Arabidopsis thaliana, Nicotiana tabacum and cassava

  • Dheeraj Mittal
  • Basanta Kumar Borah
  • Indranil DasguptaEmail author
Brief Report


Sri Lankan cassava mosaic virus (SLCMV) is a bipartite begomovirus infecting cassava in India and Sri Lanka. We have used Agrobacterium-mediated inoculation (agroinoculation) of cloned SLCMV DNA to inoculate additional hosts, Nicotiana tabacum and Arabidopsis. Although SLCMV infection in these hosts caused stunting, leaf deformation and developmental abnormalities, accumulation levels of viral DNA in the infected plants suggested that this virus was poorly adapted to them. In the natural host, cassava, agroinoculation produced infection at a low frequency. The monopartite nature of SLCMV, reported earlier in N. benthamiana, was maintained in the new hosts as well as in cassava.


Benthamiana Plant Symptomatic Plant Cassava Mosaic Disease Nucleotide Residue Biolistic Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



B. K. B. is thankful for the award of a Research Fellowship by the Council of Scientific and Industrial Research, New Delhi.


  1. 1.
    Ariyo OA, Atri GI, Dixon AGO, Winter S (2006) The use of biolistic inoculation of cassava mosaic begomovirus in screening for resistance to cassava mosaic disease. J Virol Methods 137:143CrossRefGoogle Scholar
  2. 2.
    Berrie LC, Rybicki EP, Rey MEC (2001) Complete nucleotide sequence and host range of South African cassava mosaic virus: further evidence for recombination amongst begomoviruses. J Gen Virol 82:53–58PubMedGoogle Scholar
  3. 3.
    Briddon RW, Liu S, Pinner MS, Markham PG (1998) Infectivity of African cassava mosaic virus clones to cassava by biolistic inoculation. Arch Virol 143:2487–2492PubMedCrossRefGoogle Scholar
  4. 4.
    Dutt N, Briddon RW, Dasgupta I (2005) Identification of a second begomovirus, Sri Lankan cassava mosaic virus, causing cassava mosaic disease in India. Arch Virol 150:2101–2108PubMedCrossRefGoogle Scholar
  5. 5.
    Fauquet C, Fargette D (1990) African cassava mosaic virus: etiology, epidemiology and control. Plant Dis 74:404–411CrossRefGoogle Scholar
  6. 6.
    Grimsley N, Hohn B, Hohn T, Walden R (1986) Agroinfection an alternate route for viral infection of plants by using the Ti plasmid. Proc Natl Acad Sci USA 83:3282–3286PubMedCrossRefGoogle Scholar
  7. 7.
    Jovel J, Preiss W, Jeske J (2007) Characterization of DNA intermediates of an arising geminivirus. Virus Res 130:63–70PubMedCrossRefGoogle Scholar
  8. 8.
    Lee S, Stenger DC, Bisaro DM, Davis KR (1994) Identification of loci in Arabidopsis that confer resistance to geminivirus infection. Plant J 6(4):525–535PubMedCrossRefGoogle Scholar
  9. 9.
    Malathi VG, Nair NG, Shanta P (1985) Cassava mosaic disease. Technical Bulletin Series 5, Central Tuber Crops Research Institute, Trivandrum, India, pp 18Google Scholar
  10. 10.
    Maule AJ, Hull R, Donson J (1983) The application of spot hybridisation to the detection of DNA and RNA viruses in plant tissues. J Virol Methods 6:215–224PubMedCrossRefGoogle Scholar
  11. 11.
    Park J, Hwang H, Shim H, Im K, Auh C, Lee S, Davis KR (2004) Altered cell shapes, hyperplasia and secondary growth in Arabidopsis caused by beet curly top geminivirus infection. Mol Cell 17:117–124Google Scholar
  12. 12.
    Patil BL, Rajasubramanium S, Bagchi C, Dasgupta I (2005) Both Indian cassava mosaic virus and Sri Lankan cassava mosaic virus are found in India and exhibit high variability as assessed by PCR RFLP. Arch Virol 150:389–397PubMedCrossRefGoogle Scholar
  13. 13.
    Patil BL, Dutt N, Briddon RW, Bull SE, Rothenstein D, Borah BK, Dasgupta I, Stanley J, Jeske H (2007) Deletion and recombination events between the DNA-A and DNA-B components of Indian cassava-infecting geminiviruses generate defective molecules in Nicotiana benthamiana. Virus Res 124:59–67PubMedCrossRefGoogle Scholar
  14. 14.
    Roberts CS, Rajagopal S, Smith LA (1998) A comprehensive set of modular vectors for advanced manipulations and efficient transformation of plants by both Agrobacterium and direct DNA uptake methods. CAMBIA Vector Release Manual Version 3.5Google Scholar
  15. 15.
    Rothenstein D, Briddon RW, Haible D, Stanley J, Frischmirth T, Jeske H (2005) Biolistic infection of cassava using cloned components of Indian cassava mosaic virus. Arch Virol 150:1169–1175CrossRefGoogle Scholar
  16. 16.
    Rothenstein D, Haible D, Dasgupta I, Dutt N, Patil BL, Jeske H (2006) Biodiversity and recombination of cassava-infecting begomoviruses from southern India. Arch Virol 151:55–69PubMedCrossRefGoogle Scholar
  17. 17.
    Sambrook J, Russell R (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Press, Cold Spring HarborGoogle Scholar
  18. 18.
    Saunders K, Stanley J (1999) A nanovirus-like DNA component associated with yellow vein disease of Ageratum conyzoides: evidence for interfamilial recombination between plant DNA viruses. Virology 264:142–152PubMedCrossRefGoogle Scholar
  19. 19.
    Saunders K, Salim N, Mali VR, Malathi VG, Briddon R, Markham PG, Stanley J (2002) Characterisation of Sri Lankan cassava mosaic virus and Indian cassava mosaic virus: evidence for acquisition of a DNA B component by a monopartite begomovirus. Virol 293:63–74CrossRefGoogle Scholar
  20. 20.
    Stanley J (1983) Infectivity of the cloned geminivirus genome requires sequences from both DNAs. Nature 305:643–645CrossRefGoogle Scholar
  21. 21.
    Stanley J, Gay MR (1983) Nucleotide sequence of cassava latent virus DNA. Nature 301:260–262CrossRefGoogle Scholar
  22. 22.
    Stenger DC, Revington GM, Stevenson MC, Bisaro DM (1991) Replicational release of geminivirus genomes from tandemly repeated copies, evidence for rolling circle replication of a plant viral DNA. Proc Natl Acad 88:8029–8033CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Dheeraj Mittal
    • 1
  • Basanta Kumar Borah
    • 1
  • Indranil Dasgupta
    • 1
    Email author
  1. 1.Department of Plant Molecular BiologyUniversity of Delhi South CampusNew DelhiIndia

Personalised recommendations