Archives of Virology

, Volume 153, Issue 11, pp 1991–1997

Hepatitis C virus (HCV) NS2 protein up-regulates HCV IRES-dependent translation and down-regulates NS5B RdRp activity

  • Yinglong She
  • Qingjiao Liao
  • Xulin Chen
  • Linbai Ye
  • Zhenghui Wu
Original Article
  • 144 Downloads

Abstract

Chronic hepatitis C virus (HCV) infection often leads to liver cancer. The HCV NS2 protein is a hydrophobic transmembrane protein that associates with several cellular proteins in mammalian cells. In this report, we investigated the function of NS2 protein on HCV replication and translation by using a transient cell-based expression system. Cells co-transfected with pcDNA3.1 (−)-NS2 and the dual-luciferase reporter construct containing the HCV IRES were used to detect the effect of NS2 protein on HCV translation. Cells co-transfected with pcDNA3.1(−)-NS2, pcDNA-NS5B and a reporter plasmid were used to detect the effect of NS2 protein on HCV replication. The results showed that HCV NS2 protein up-regulated HCV IRES-dependent translation in a specific and dose-dependent manner in Huh7 cells but not in HeLa and HepG2 cells, and NS2 protein inhibited NS5B RdRp activity in a dose-independent manner in all three cell lines. These findings may suggest a novel mechanism by which HCV modulates its NS5B replication and IRES-dependent translation and facilitates virus persistence.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Yinglong She
    • 1
  • Qingjiao Liao
    • 2
  • Xulin Chen
    • 2
  • Linbai Ye
    • 1
  • Zhenghui Wu
    • 1
  1. 1.State Key Laboratory of Virology, College of Life ScienceWuhan UniverisityWuhanPeople’s Republic of China
  2. 2.State Key Laboratory of VirologyWuhan Institute of Virology, Chinese Academic of ScienceWuhanPeople’s Republic of China

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