Alternate hosts of African cassava mosaic virus and East African cassava mosaic Cameroon virus in Nigeria
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Cassava mosaic disease (CMD) caused by African cassava mosaic virus (ACMV) and East African cassava mosaic Cameroon virus (EACMCV) is the major constraint to cassava production in Nigeria. Sequences of the DNA-A component of ACMV and EACMCV isolates from leguminous plant species (Senna occidentalis, Leucana leucocephala and Glycine max), castor oil plant (Ricinus communis), a weed host (Combretum confertum) and a wild species of cassava (Manihot glaziovii) were determined. All ACMV isolates from these hosts showed 96–98% nucleotide sequence identity with cassava isolates from West Africa. EACMCV was found only in four hosts (S. occidentalis, L. leucocephala, C. confertum, M. glaziovii), and sequences of these isolates showed 96–99% identity with cassava isolates from West Africa. These results provide definitive evidence for the natural occurrence of ACMV and EACMCV in plant species besides cassava.
KeywordsNucleotide Sequence Identity Cassava Mosaic Disease Cassava Production African Cassava Mosaic Virus Savannah Zone
This work was supported in part by US Agency for International Development (USAID) Linkage grant from International Institute of Tropical Agriculture to Washington State University. PPNS #0493, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences Agricultural Research Center Project No. WNPO 0616, Washington State University, Pullman, WA 99164-6240, USA. DNA samples used in this study were obtained with permission from USDA-APHIS-PPQ (permit number P526P-07-06707).
- 1.Alabi OJ, Ogbe FO, Bandyopadhyay R, Dixon AGO, Hughes J, Naidu RA (2007) The occurrence of African cassava mosaic virus and East African cassava mosaic Cameroon virus in natural hosts other than cassava in Nigeria. Phytopathology 97:S3Google Scholar
- 10.Mgbechi-Ezeri JU, Alabi OJ, Naidu RA, Lava Kumar P (2008) First report of the occurrence of African cassava mosaic virus in soybean in Nigeria. Plant Dis. (PD-05-08-0317-PDN)Google Scholar
- 11.Ogbe FO, Atiri GI, Dixon AGO, Thottappilly G (2001) Cassava mosaic disease and its causal agents: the Nigerian experience. Proceedings of the 1st Conference of Plant Virology in Sub-Saharan Africa, IITA, Ibadan, Nigeria, 4–8 June 2001Google Scholar
- 13.Rossel HW, Thottappilly G, Van Lent JMW, Huttinga H (1987) The etiology of cassava mosaic in Nigeria. In: Fauquet C, Fargette D (eds) Proc. Intern. Seminar African Cassava Mosaic Disease and its Control. 4–8 May 1987. CTA, FAO, ORSTOM, IITA, IAPC publication, Yamoussoukro; Cote d’Ivoire, pp 43–56Google Scholar
- 14.Shoyinka SA, Thottappilly G, McGrath FF, Harrison BD (2001) Detection, relationships and properties of cassava mosaic geminivirus in naturally infected castor oil plant, Ricinus communis L. in Nigeria. Page 20 In: The book of abstracts, Fifth Conf. Cassava Biotech. Network (CBN-V), Donald Danforth Plant Science Center, St. Louis, Missouri, USA, 4–9 November 2001. pp S8–S20Google Scholar
- 16.Swanson MM, Harrison BD (1994) Properties, relationships and distribution of cassava mosaic geminiviruses. Trop Sci 34:15–25Google Scholar
- 18.Thresh JM, Otim-Nape GW, Legg JP, Fargette D (1997) African cassava mosaic virus disease: the magnitude of the problem. AJRTC 2:13–19Google Scholar