Archives of Virology

, Volume 153, Issue 9, pp 1677–1684

GTPase activity is not essential for the interferon-inducible MxA protein to inhibit the replication of hepatitis B virus

  • Zhijian Yu
  • Zhanhui Wang
  • Jinjun Chen
  • Hui Li
  • Zhanzhou Lin
  • Fan Zhang
  • Yuanping Zhou
  • Jinlin Hou
Original Article


Multiple studies have established that GTPase activity is critical for MxA to act against RNA viruses. Recently, it was shown that MxA can also restrict the replication of hepatitis B virus (HBV), a DNA virus, but the requirements for GTPase activity in inhibition of HBV by MxA remain unknown. Here, we report that GTPase-defective mutants (K83A, T103A, and L612K) can downregulate extracellullar HBsAg and HBeAg and reduce the expression of extra- and intracellular HBV DNA in HepG2 cells to levels similar to that achieved by wild-type MxA. Furthermore, TMxA and T103, two nuclear forms of wild-type MxA and a GTPase-defective mutant (T103A) could only slightly decrease the expression of extra- and intracellular HBV DNA in HepG2 cells. In conclusion, GTPase activity is not essential for MxA protein to inhibit HBV replication, and MxA may have only a minimal effect on the replicative cycle of HBV in the nucleus.


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Zhijian Yu
    • 1
  • Zhanhui Wang
    • 1
  • Jinjun Chen
    • 1
  • Hui Li
    • 1
  • Zhanzhou Lin
    • 1
  • Fan Zhang
    • 1
  • Yuanping Zhou
    • 1
  • Jinlin Hou
    • 1
    • 2
  1. 1.Department of Infectious DiseasesNanfang Hospital, Nanfang Medical UniversityGuangzhouChina
  2. 2.GuangzhouChina

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