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Archives of Virology

, Volume 153, Issue 8, pp 1479–1488 | Cite as

Definition of subtypes in the European genotype of porcine reproductive and respiratory syndrome virus: nucleocapsid characteristics and geographical distribution in Europe

  • Tomasz StadejekEmail author
  • Martin B. Oleksiewicz
  • Alexei V. Scherbakov
  • Anna M. Timina
  • Jonas S. Krabbe
  • Katarzyna Chabros
  • Denis Potapchuk
Original Article

Abstract

The nucleocapsid protein of the European genotype of porcine reproductive and respiratory syndrome virus (type 1, PRRSV-1) exhibited extensive size polymorphism (124–130 amino acids), correlating with phylogenetic grouping of ORF7 as well as ORF5 nucleotide sequences, thereby validating ORF7 size as an independent PRRSV-1 subtype marker. Based on new sequence information from the Russian Federation, we propose division of European genotype PRRSV-1 into 3 subtypes: a pan-European subtype 1 and East European subtypes 2 and 3, with nucleocapsid protein sizes of 128, 125 and 124 amino acids, respectively. The genetic differences between European genotype PRRSV subtypes affected diagnostic RT-PCR primer binding sites. Using Escherichia coli-expressed ORF7 protein, we confirmed that even the relatively closely related PRRSV subtypes 2 and 3 were antigenically different. Finally, the isoelectric point (pI) correlated with the nucleocapsid protein size for European genotype PRRSV subtypes, suggesting subtype-specific compensatory structural changes associated with subtype-specific ORF7 sizes. Thus, the new ORF7-based subtype division of PRRSV-1 proposed here is biologically meaningful and practically relevant.

Keywords

Nucleocapsid Protein Respiratory Syndrome Virus ORF7 Protein Lelystad Virus European Genotype 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Supplementary material

705_2008_146_MOESM1_ESM.doc (254 kb)
Supplementary Table S1. Sequence summary (DOC 254 kb)

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Tomasz Stadejek
    • 1
    Email author
  • Martin B. Oleksiewicz
    • 2
  • Alexei V. Scherbakov
    • 3
  • Anna M. Timina
    • 3
  • Jonas S. Krabbe
    • 2
  • Katarzyna Chabros
    • 1
  • Denis Potapchuk
    • 4
  1. 1.National Veterinary Research Institute, Department of Swine DiseasesOIE Reference Laboratory for PRRSPuławyPoland
  2. 2.Novo Nordisk A/S, Molecular ToxicologyMåløvDenmark
  3. 3.Federal Centre for Animal Health (FGI “ARRIAH”)OIE Collaborating Center for Diagnosis and Control of Animal Diseases in Eastern Europe, Central Asia and TranscaucasiaYur’evetsRussia
  4. 4.S.N. Vyshelesskij Institute of Experimental Veterinary InstituteNational Academy of Sciences of BelarusMinskBelarus

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