Archives of Virology

, Volume 153, Issue 7, pp 1401–1406 | Cite as

Effect of siRNA on HSV-1 plaque formation and relative expression levels of UL39 mRNA

  • Ren Zhe
  • Zhang Mei-Ying
  • Kaio Kitazato
  • Nobuyuki Kobayash
  • Zhu Qin-Chang
  • Zhang Pei-Zhuo
  • Yang Zhi-Rong
  • Wang Yi-Fei
Brief Report
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Abstract

RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. In this study, we applied siRNAs targeting the UL39 gene of human herpes simplex virus type 1 (HSV-1), which encodes the large subunit of ribonucleotide reductase, ICP6. Using an ICP6 expression reporter plasmid and an in vitro model of infection, we showed that synthetic siRNA silenced effectively and specifically UL39 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.

Keywords

Vero Cell Ribonucleotide Reductase UL39 Gene EGFP Reporter Gene Ribonucleotide Reductase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

This work was supported by grants from the Program of Tackling Industry, Science and Technology Problems, Guangdong Province, China (No. 2005A10904001), the United Grant of the National and Local Natural Science Foundation of China (No. U0632010) and the Key Laboratory Open Grant of Jinan University (No. 2005). We thank Shanghai GenePharma Co., Ltd. for their help.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Ren Zhe
    • 1
    • 2
  • Zhang Mei-Ying
    • 2
  • Kaio Kitazato
    • 3
  • Nobuyuki Kobayash
    • 3
  • Zhu Qin-Chang
    • 2
  • Zhang Pei-Zhuo
    • 4
  • Yang Zhi-Rong
    • 1
    • 5
  • Wang Yi-Fei
    • 2
    • 5
  1. 1.College of Life Sciences of Sichuan UniversityChengduChina
  2. 2.Biomedicine Research and Development Center of Jinan UniversityGuangzhouChina
  3. 3.Laboratory of Molecular Biology of Infectious Agents, Department of Molecular Microbiology and Immunology, Graduate School of Biomedical SciencesNagasaki UniversityNagasakiJapan
  4. 4.Shanghai GenePharma Co., LtdShanghaiChina
  5. 5.Biomedicine Research and Development Center of Jinan UniversityGuangzhouChina

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