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Archives of Virology

, 153:1185 | Cite as

Characterization of Hawaiian isolates of Cymbidium mosaic virus (CymMV) co-infecting Dendrobium orchid

  • S. P. VaughanEmail author
  • M. Grisoni
  • M. H. Kumagai
  • A. R. Kuehnle
Brief Report

Abstract

We report here the isolation and characterization of three distinct isolates of Cymbidium mosaic virus (CymMV) co-infecting Dendrobium orchid in Hawaii. Isolates 1 and 2 were phylogenetically distinct from previously reported CymMV isolates. However, isolate 3 was highly similar to previously reported CymMV sequences and could be localised to CymMV subgroup A. Isolate 2 localised to CymMV subgroup B. Thus, we report here the first full-length CymMV subgroup B isolate. Isolate 1 represents a recombination event between isolates 2 and 3. Infectivity assays revealed that all three isolates are functional and individually infectious in both Dendrobium and indicator species.

Keywords

Coat Protein Coat Protein Gene Triple Gene Block Coat Protein Region Cymbidium Mosaic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported by the UDSA Special Grants Program for Tropical and Subtropical Agriculture Research, grant number HAW00848-1015S. We would also like to thank Dr. T. Amore for provision of plant material, Pierre Lefeuvre for helpful comments and guidance in utilisation of the RDP3 software and Dr. A.D. Hieber for helpful discussions.

References

  1. 1.
    Mudalige RG, Kuehnle AR (2004) Orchid biotechnology in production and improvement. Hortscience 39:11–17Google Scholar
  2. 2.
    Hu JS, Ferreira S, Wang M, Xu MQ (1993) Detection of Cymbidium mosaic-virus, Odontoglossum ringspot virus, Tomato spotted wilt virus and Potyviruses infecting Orchids in Hawaii. Plant Dis 77:464–468Google Scholar
  3. 3.
    Wong SM, Chng CG, Lee YH, Tan K, Zettler FW (1994) Incidence Of Cymbidium Mosaic and Odontoglossum Ringspot Viruses and their significance in orchid cultivation in Singapore. Crop Prot 13:235–239CrossRefGoogle Scholar
  4. 4.
    Wong SM, Mahtani PH, Lee KC, Yu HH, Tan Y, Neo KK, Chan Y, Wu M, Chng CG (1997) Cymbidium mosaic potexvirus RNA: complete nucleotide sequence and phylogenetic analysis—Brief report. Arch Virol 142:383–391PubMedCrossRefGoogle Scholar
  5. 5.
    Wagner B, Fuchs H, Adhami F, Ma Y, Scheiner O, Breiteneder H (2004) Plant virus expression systems for transient production of recombinant allergens in Nicotiana benthamiana. Methods 32:227–234PubMedCrossRefGoogle Scholar
  6. 6.
    Champagne MM, Kuehnle AR (2000) An effective method for isolating RNA from tissues of Dendrobium. Lindleyana 15:165–168Google Scholar
  7. 7.
    White KA, Bancroft JB, Mackie GA (1992) Mutagenesis of a hexanucleotide sequence conserved in Potexvirus RNAs. Virology 189:817–820PubMedCrossRefGoogle Scholar
  8. 8.
    Chen J, Shi YH, Adams MJ, Chen JP (2005) The complete sequence of the genomic RNA of an isolate of Lily virus X (genus Potexvirus). Arch Virol 150:825–832PubMedCrossRefGoogle Scholar
  9. 9.
    Zuidema D, Linthorst HJM, Huisman MJ, Asjes CJ, Bol JF (1989) Nucleotide sequence of Narcissus mosaic-virus RNA. J Gen Virol 70:267–276PubMedGoogle Scholar
  10. 10.
    Ajjikuttira PA, Lim-Ho CL, Woon MH, Ryu KH, Chang CA, Loh CS, Wong SM (2002) Genetic variability in the coat protein genes of two orchid viruses: Cymbidium mosaic virus and Odontoglossum ringspot virus. Arch Virol 147:1943–1954PubMedCrossRefGoogle Scholar
  11. 11.
    Sherpa AR, Hallan V, Pathak P, Zaidi AA (2006) Characterization of the coat protein gene of Cymbidium mosaic virus isolates from India. J Phytopathol 154:275–280CrossRefGoogle Scholar
  12. 12.
    Adams MJ, Antoniw JF, Bar-Joseph M, Brunt AA, Candresse T, Foster GD, Martelli GP, Milne RG, Zavriev SK, Fauquet CM (2004) The new plant virus family Flexiviridae and assessment of molecular criteria for species demarcation. Arch Virol 149:1045–1060PubMedGoogle Scholar
  13. 13.
    Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  14. 14.
    Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599PubMedCrossRefGoogle Scholar
  15. 15.
    Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
  16. 16.
    Moles M, Delatte H, Farreyrol K, Grisoni M (2007) Evidence that Cymbidium mosaic virus (CymMV) isolates divide into two subgroups based on nucleotide diversity of coat protein and replicase genes. Arch Virol 152:705–715PubMedCrossRefGoogle Scholar
  17. 17.
    Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  18. 18.
    Martin DP, Williamson C, Posada D (2005) RDP2: recombination detection and analysis from sequence alignments. Bioinformatics 21:260–262PubMedCrossRefGoogle Scholar
  19. 19.
    Martin D, Rybicki E (2000) RDP: detection of recombination amongst aligned sequences. Bioinformatics 16:562–563PubMedCrossRefGoogle Scholar
  20. 20.
    Padidam M, Sawyer S, Fauquet CM (1999) Possible emergence of new geminiviruses by frequent recombination. Virology 265:218–225PubMedCrossRefGoogle Scholar
  21. 21.
    Maynard Smith J (1992) Analyzing the mosaic structure of genes. J Mol Evol 34:126–129Google Scholar
  22. 22.
    Martin DP, Posada D, Crandall KA, Williamson C (2005) A modified bootscan algorithm for automated identification of recombinant sequences and recombination breakpoints. AIDS Res Hum Retroviruses 21:98–102PubMedCrossRefGoogle Scholar
  23. 23.
    Gibbs MJ, Armstrong JS, Gibbs AJ (2000) Sister-scanning: a Monte Carlo procedure for assessing signals in recombinant sequences. Bioinformatics 16:573–582PubMedCrossRefGoogle Scholar
  24. 24.
    Posada D, Crandall KA (2001) Evaluation of methods for detecting recombination from DNA sequences: computer simulations. Proc Natl Acad Sci USA 98:13757–13762PubMedCrossRefGoogle Scholar
  25. 25.
    Boni MF, Posada D, Feldman MW (2007) An exact nonparametric method for inferring mosaic structure in sequence triplets. Genetics 176:1035–1047PubMedCrossRefGoogle Scholar
  26. 26.
    Holmes EC, Worobey M, Rambaut A (1999) Phylogenetic evidence for recombination in dengue virus. Mol Biol Evol 16:405–409PubMedGoogle Scholar
  27. 27.
    Sherpa A, Hallan V, Pathak P, Zaidi A (2007) Complete nucleotide sequence analysis of Cymbidium mosaic virus Indian isolate: further evidence for natural recombination among potexviruses. J Biosci 32:663–669PubMedCrossRefGoogle Scholar
  28. 28.
    Fernandez-Delmond I, Pierrugues O, M Wispelaere, Guilbaud L, Gaubert S, Diveki Z, Godon C, Tepfer M, Jacquemond M (2004) A novel strategy for creating recombinant infectious RNA virus genomes. J Virol Methods 121:247–257PubMedCrossRefGoogle Scholar
  29. 29.
    Yu W, Rusterholtz KJ, Krummel AT, Lehman N (2006) Detection of high levels of recombination generated during PCR amplification of RNA templates. Biotechniques 40:499–507PubMedCrossRefGoogle Scholar
  30. 30.
    Chare ER, Holmes EC (2006) A phylogenetic survey of recombination frequency in plant RNA viruses. Arch Virol 151:933–946PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • S. P. Vaughan
    • 1
    • 2
    Email author
  • M. Grisoni
    • 3
  • M. H. Kumagai
    • 1
  • A. R. Kuehnle
    • 1
  1. 1.Department of Tropical Plant and Soil SciencesUniversity of HawaiiHonoluluUSA
  2. 2.Rothamsted ResearchHarpenden, HertsUK
  3. 3.CIRAD, UMR CIRAD-UR “Peuplements Végétaux et Bioagresseurs en Milieu Tropical”, Pôle de Protection des PlantesSaint PierreFrance

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