Characterization of Hawaiian isolates of Cymbidium mosaic virus (CymMV) co-infecting Dendrobium orchid
- 190 Downloads
We report here the isolation and characterization of three distinct isolates of Cymbidium mosaic virus (CymMV) co-infecting Dendrobium orchid in Hawaii. Isolates 1 and 2 were phylogenetically distinct from previously reported CymMV isolates. However, isolate 3 was highly similar to previously reported CymMV sequences and could be localised to CymMV subgroup A. Isolate 2 localised to CymMV subgroup B. Thus, we report here the first full-length CymMV subgroup B isolate. Isolate 1 represents a recombination event between isolates 2 and 3. Infectivity assays revealed that all three isolates are functional and individually infectious in both Dendrobium and indicator species.
KeywordsCoat Protein Coat Protein Gene Triple Gene Block Coat Protein Region Cymbidium Mosaic Virus
This research was supported by the UDSA Special Grants Program for Tropical and Subtropical Agriculture Research, grant number HAW00848-1015S. We would also like to thank Dr. T. Amore for provision of plant material, Pierre Lefeuvre for helpful comments and guidance in utilisation of the RDP3 software and Dr. A.D. Hieber for helpful discussions.
- 1.Mudalige RG, Kuehnle AR (2004) Orchid biotechnology in production and improvement. Hortscience 39:11–17Google Scholar
- 2.Hu JS, Ferreira S, Wang M, Xu MQ (1993) Detection of Cymbidium mosaic-virus, Odontoglossum ringspot virus, Tomato spotted wilt virus and Potyviruses infecting Orchids in Hawaii. Plant Dis 77:464–468Google Scholar
- 6.Champagne MM, Kuehnle AR (2000) An effective method for isolating RNA from tissues of Dendrobium. Lindleyana 15:165–168Google Scholar
- 17.Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
- 21.Maynard Smith J (1992) Analyzing the mosaic structure of genes. J Mol Evol 34:126–129Google Scholar