Archives of Virology

, 153:1185 | Cite as

Characterization of Hawaiian isolates of Cymbidium mosaic virus (CymMV) co-infecting Dendrobium orchid

  • S. P. VaughanEmail author
  • M. Grisoni
  • M. H. Kumagai
  • A. R. Kuehnle
Brief Report


We report here the isolation and characterization of three distinct isolates of Cymbidium mosaic virus (CymMV) co-infecting Dendrobium orchid in Hawaii. Isolates 1 and 2 were phylogenetically distinct from previously reported CymMV isolates. However, isolate 3 was highly similar to previously reported CymMV sequences and could be localised to CymMV subgroup A. Isolate 2 localised to CymMV subgroup B. Thus, we report here the first full-length CymMV subgroup B isolate. Isolate 1 represents a recombination event between isolates 2 and 3. Infectivity assays revealed that all three isolates are functional and individually infectious in both Dendrobium and indicator species.


Coat Protein Coat Protein Gene Triple Gene Block Coat Protein Region Cymbidium Mosaic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was supported by the UDSA Special Grants Program for Tropical and Subtropical Agriculture Research, grant number HAW00848-1015S. We would also like to thank Dr. T. Amore for provision of plant material, Pierre Lefeuvre for helpful comments and guidance in utilisation of the RDP3 software and Dr. A.D. Hieber for helpful discussions.


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • S. P. Vaughan
    • 1
    • 2
    Email author
  • M. Grisoni
    • 3
  • M. H. Kumagai
    • 1
  • A. R. Kuehnle
    • 1
  1. 1.Department of Tropical Plant and Soil SciencesUniversity of HawaiiHonoluluUSA
  2. 2.Rothamsted ResearchHarpenden, HertsUK
  3. 3.CIRAD, UMR CIRAD-UR “Peuplements Végétaux et Bioagresseurs en Milieu Tropical”, Pôle de Protection des PlantesSaint PierreFrance

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