Archives of Virology

, Volume 153, Issue 5, pp 855–864

Characterization of tomato zonate spot virus, a new tospovirus in China

  • Jia-Hong Dong
  • Xiao-Fei Cheng
  • Yue-Yan Yin
  • Qi Fang
  • Ming Ding
  • Ting-Ting Li
  • Li-Zhen Zhang
  • Xiao-Xia Su
  • Jenifer Huang McBeath
  • Zhong-Kai Zhang
Original Article


An isolate of a new tospovirus species, causing concentric zoned ringspots on fruits and necrotic lesions on leaves of infected plants, was characterised based on particle morphology, host range and serological properties. The complete nucleotide sequences of large (L), medium (M), and small (S) RNAs of this virus were found to contain 8919, 4945, and 3279 nts respectively. The L RNA encoded the RNA-dependent RNA polymerase (RdRp) (2885 aa, 332.7 kDa). The M RNA encoded a non-structural (NSm) protein (309 aa, 34.4 kDa) and a viral glycoprotein precursor (Gn/Gc) (1122 aa, 127.4 kDa). The S RNA encoded a non-structural protein (NSs) (459 aa, 51.9 kDa) and the nucleocapsid (N) protein (278 aa, 30.6 kDa). This N protein shared amino acid identities of 80.9% with those of calla lily chlorotic spot virus. Our results suggest that the virus studied here belongs to a new tospovirus species, for which the name tomato zonate spot virus is proposed.


  1. 1.
    Bendtsen JD, Nielsen H, von Heijne G, Brunak S (2004) Improved prediction of signal peptides—SignalP 3.0. J Mol Biol 340:783–795PubMedCrossRefGoogle Scholar
  2. 2.
    Bezerra IC, Resende RO, Pozzer L, Nagata T, Kormelink R, de Avila AC (1999) Increase of tospoviral diversity in Brazil, with the identification of two new tospovirus species, one from Chrysanthemum and another from Zucchini. Phytopathology 89:823–830CrossRefPubMedGoogle Scholar
  3. 3.
    Brittlebank CC (1919) Tomato diseases. J Agric Victoria 17:231–235Google Scholar
  4. 4.
    Bruenn JA (2003) A structural and primary sequence comparison of the viral RNA-dependent RNA polymerases. Nucleic Acids Res 31:1821–1829PubMedCrossRefGoogle Scholar
  5. 5.
    Chen CC, Chen TC, Lin YH, Yeh SD, Hsu HT (2005) A chlorotic spot disease on calla lilies (Zantedeschia spp.) is caused by a tospovirus serologically but distantly related to Watermelon silver mottle virus. Plant Dis 89:440–445CrossRefGoogle Scholar
  6. 6.
    Chen CC, Chiu RJ (1996) A tospovirus infecting peanut in Taiwan. Acta Hortic 431:57–67Google Scholar
  7. 7.
    Chen K, Xu Z, Yan L, Wang G (2007) Characterization of a new strain of Capsicum chlorosis virus from peanut (Arachis hypogaea L.) in China. J Phytopathol 155:178–181CrossRefGoogle Scholar
  8. 8.
    Chu FH, Yeh SD (1998) Comparison of ambisense M RNA of Watermelon silver mottle virus with other tospoviruses. Phytopathology 88:351–358CrossRefPubMedGoogle Scholar
  9. 9.
    Chu FH, Chao CH, Chung MH, Chen CC, Yeh SD (2001) Completion of the genome sequence of Watermelon silver mottle virus and utilization of degenerate primers for detecting tospoviruses in five serogroups. Phytopathology 91:361–368CrossRefPubMedGoogle Scholar
  10. 10.
    Chu FH, Chao CH, Peng YC, Lin SS, Chen CC, Yeh SD (2001) Serological and molecular characterization of Peanut chlorotic fan-spot virus, a new species of the genus Tospovirus. Phytopathology 91:856–863CrossRefPubMedGoogle Scholar
  11. 11.
    Cortês I, Livieratos J, Derks A, Peters D, Kormelink R (1998) Molecular and serological characterization of Iris yellow spot virus, a new and distinct tospovirus species. Phytopathology 88:1276–1282CrossRefPubMedGoogle Scholar
  12. 12.
    Cortez I, Saaijer J, Wongjkaew KS, Pereira AM, Goldbach R, Peters D, Kormelink R (2001) Identification and characterization of a novel tospovirus species using a new RT-PCR approach. Arch Virol 146:265–278PubMedCrossRefGoogle Scholar
  13. 13.
    De Avila AC, de Haan P, Kormelink R, Resende RO, Goldbach RW, Peters D (1993) Classification of tospoviruses based on phylogeny of nucleoprotein gene sequences. J Gen Virol 74:153–159PubMedGoogle Scholar
  14. 14.
    De Haan P, de Avila AC, Kormelink R, Westerbroek A, Gielen JJL, Peters D, Goldbach RW (1992) The nucleotide sequence of the S RNA of impatiens necrotic spot virus, a novel tospovirus. FEBS Lett 306:27–32PubMedCrossRefGoogle Scholar
  15. 15.
    De Haan P, Kormelink R, Resende RO, van Poelwijk F, Peters D, Goldbach R (1991) Tomato spotted wilt virus L RNA encodes a putative RNA polymerase. J Gen Virol 71:2207–2216CrossRefGoogle Scholar
  16. 16.
    De Haan P,Wagemakers L, Peters D, Goldbach R (1990) The S RNA segment of tomato spotted wilt virus has an ambisense character. J Gen Virol 71:1001–1007PubMedGoogle Scholar
  17. 17.
    Ding M, Luo YQ, Fang Q, Zhang ZK, Zhao ZW (2007) First report of Groundnut yellow spot virus infecting Capsicum annuum in China. J Plant Pathol 89(2):295Google Scholar
  18. 18.
    Elliot RM (1990) Molecular biology of the Bunyaviridae. J Gen Virol 71:501–522Google Scholar
  19. 19.
    German TL, Ullman DE, Moyer JW (1992) Tospoviruses: diagnosis, molecular biology, phylogeny, and vector relationships. Annu Rev Phytopathol 30:315–348CrossRefPubMedGoogle Scholar
  20. 20.
    Gowda S, Satyanarayana T, Naidu RA, Mushegian A, Dawson WO, Reddy DVR (1998) Characterization of the large (L) RNA of peanut bud necrosis tospovirus. Arch Virol 143:2381–2390PubMedCrossRefGoogle Scholar
  21. 21.
    Hassani-Mehraban A, Saaijer J, Peters D, Goldbach R, Kormelink R(2005) A new tomato infecting tospovirus from Iran. Phytopathology 95:852–858CrossRefPubMedGoogle Scholar
  22. 22.
    Jain RK, Pappu HR, Pappu SS, Reddy MK, Vani A (1998) Watermelon bud necrosis tospovirus is a distinct virus species belonging to serogroup IV. Arch Virol 143:1637–1644PubMedCrossRefGoogle Scholar
  23. 23.
    Jones DR (2005) Plant viruses transmitted by thrips. Eur J Plant Pathol 113:119–157CrossRefGoogle Scholar
  24. 24.
    Julenius K, Mølgaard A, Gupta R, Brunak S (2005) Prediction, conservation analysis and structural characterization of mammalian mucin-type O-glycosylation sites. Glycobiology 15:153–164PubMedCrossRefGoogle Scholar
  25. 25.
    Kato K, Hanada K (2000) Characterization of the S RNA segment of melon yellow spot virus. Jpn J Phytopathol 66:252–254Google Scholar
  26. 26.
    Kato K, Hanada K, Kameya-Iwaki M (2000) Melon yellow spot virus: a distinct species of the genus Tospovirus isolated from melon. Phytopathology 90:422–426CrossRefPubMedGoogle Scholar
  27. 27.
    Knierim D, Blawid R, Maiss E (2006) The complete nucleotide sequence of a Capsicum chlorosis virus isolate from Lycopersicum esculentum in Thailand. Arch Virol 151:1761–1782PubMedCrossRefGoogle Scholar
  28. 28.
    Kormelink R, de Haan P, Meurs C, Peters D, Goldbach R (1992) The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments. J Gen Virol 73:2795–2804PubMedGoogle Scholar
  29. 29.
    Krogh A, Larsson B, von Heijne G, Sonnhammer ELL (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 305:567–580PubMedCrossRefGoogle Scholar
  30. 30.
    Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163PubMedCrossRefGoogle Scholar
  31. 31.
    Law MD, Speck J, Moyer JW (1991) Nucleotide sequence of the 3-non-coding region and N gene of the S RNA of a serologically distinct tospovirus. J GenVirol 72:2597–2601Google Scholar
  32. 32.
    Law MD, Speck J, Moyer JW (1992) The M RNA of impatiens necrotic spot Tospovirus (Bunyaviridae) has an ambisense genomic organization. Virology 188:732–741PubMedCrossRefGoogle Scholar
  33. 33.
    Li YQ, Zhang ZK, Guan SJ, Pen HM, Li JY, Zou YJ (1997) Kinds of tobacco viral pathogens and the infection cycle at Binchuan county. J Yunnan Agric Univ 12:263–268 (In Chinese with English summary)Google Scholar
  34. 34.
    Lin YH, Chen TC, Hsu HT, Liu FL, Chu FH, Chen CC, Lin YZ, Yeh SD (2005) Serological comparison and molecular characterization for verification of Calla lily chlorotic spot virus as a new tospovirus species belonging to Watermelon silver mottle virus serogroup. Phytopathology 95:1482–1488CrossRefPubMedGoogle Scholar
  35. 35.
    McMichael LA, Persley DM, Thomas JE (2002) A new tospovirus serogroup IV species infecting capsicum and tomato in Queensland, Australia. Australasian Plant Pathol 31:231–239CrossRefGoogle Scholar
  36. 36.
    Nichol ST, Beaty BJ, Elliott RM, Goldbach R, Plyusnin A, Schmaljohn CS, Tesh RB (2005) Bunyaviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus Taxonomy, VIIIth Report of the ICTV Elsevier/Academic Press, San Diego, pp 695–716Google Scholar
  37. 37.
    Okuda M, Kato K, Hanada K, Iwanami T (2004) Complete nucleotide sequence of L RNA segment of melon yellow spot virus. Jpn J Phytopathol 70:14–17Google Scholar
  38. 38.
    Okuda M, Kato K, Hanada K, Iwanami T (2006) Nucleotide sequence of melon yellow spot virus M RNA segment and characterization of non-viral sequences in subgenomic RNA. Arch Virol 151:1–11PubMedCrossRefGoogle Scholar
  39. 39.
    Pappu SS, Bhat AI, Pappu HR, Deom CM, Culbreath AK (2000) Phylogenetic studies of tospoviruses (family: Bunyaviridae) based on intergenic region sequences of small and medium genomic RNAs. Arch Virol 145:1035–1045PubMedCrossRefGoogle Scholar
  40. 40.
    Persley DM, Thomas JE, Sharman M (2006) Tospoviruses—an Australian perspective. Australasian Plant Pathol 35:161–180CrossRefGoogle Scholar
  41. 41.
    Reddy DVR, Ratna AS, Sudarshana MR, Poul F, Kumar IK (1992) Serological relationships and purification of bud necrosis virus, a tospovirus occurring in peanut (Arachis hypogaea L.) in India. Ann Appl Biol 120:279–286CrossRefGoogle Scholar
  42. 42.
    Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Laboratory Press, New YorkGoogle Scholar
  43. 43.
    Satyanarayana T, Gowda S, Reddy KL, Mitchell SE, Dawson WO, Reddy DVR (1998) Peanut yellow spot virus is a member of a new serogroup of tospovirus genus based on small (S) RNA sequence and organization. Arch Virol 143:353–364PubMedCrossRefGoogle Scholar
  44. 44.
    Satyanarayana T, Mitchell SE, Reddy DVR, Brown S, Kresovich S, Jarret R, Naidu RA, Demski JW (1996) Peanut bud necrosis tospovirus S RNA: complete nucleotide sequence, genome organization and homology to other tospoviruses. Arch Virol 141:85–98PubMedCrossRefGoogle Scholar
  45. 45.
    Satyanarayana T, Mitchell SE, Reddy DVR, Kresovich S, Jarret R, Naidu RA, Gowda S, Demski JW (1996) The complete nucleotide sequence and genome organization of the RNA segment of peanut bud necrosis tospovirus and comparison with other tospoviruses. J Gen Virol 77:2347–2352PubMedGoogle Scholar
  46. 46.
    Sherwood JL, German TL, Whitfield AE, Moyer JW, Ullman DE (2000) Tomato spotted wilt. In: Maloy OC, Murray TD (eds) Encyclopedia of plant pathology. Wiley, New York, pp 1034–1040Google Scholar
  47. 47.
    Singh SJ, Krishnareddy M (1996) Watermelon bud necrosis: a new tospovirus disease. Acta Hortic 431:68–77Google Scholar
  48. 48.
    Su DK, Yuan XZ, Xie YH, Wang SR, Ding H (1987) Tomato spotted wilt virus in tomato in Chengdu and Dukou. Acta Phytopathologica Sinica 17(4):255–256 (In Chinese)Google Scholar
  49. 49.
    Van Poelwijk F, Prins M, Goldbach R (1997) Completion of the impatiens necrotic spot virus genome sequence and genetic comparison of the l proteins within the family Bunyaviridae. J Gen Virol 78:543–546PubMedGoogle Scholar
  50. 50.
    Whitfield AE, Ullman DE, German TL (2005) Tospovirus-thrips interactions. Ann Rev Phytopathol 43:459–489CrossRefGoogle Scholar
  51. 51.
    Yao G (1992) Tomato spotted wilt virus in sun-cured tobacco in Sichuan. Tobacco Sci Technol 6:39–40 (In Chinese)Google Scholar
  52. 52.
    Yeh S, Sun I, Ho H, Chang T (1996) Molecular cloning and nucleotide sequence analysis of the S RNA of watermelon silver mottle tospovirus. Acta Hortic 431:244–260Google Scholar
  53. 53.
    Yeh SD, Chang TF (1995) Nucleotide sequence of the N gene of Watermelon silver mottle virus, a proposed new member of the genus Tospovirus. Phytopathology 85:58–64CrossRefGoogle Scholar
  54. 54.
    Zhang ZK, Ding M, Fang Q, Zhang LZ, Li TT, Peng LB, Su XX, Li Z (2004) The preliminary study of the occurrence and distribution of tospoviruses in Yunnan. Southwest China J Agric Sci 17(z1):163–168 (In Chinese with English summary)Google Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jia-Hong Dong
    • 1
  • Xiao-Fei Cheng
    • 1
  • Yue-Yan Yin
    • 1
  • Qi Fang
    • 1
  • Ming Ding
    • 1
  • Ting-Ting Li
    • 1
  • Li-Zhen Zhang
    • 1
  • Xiao-Xia Su
    • 1
  • Jenifer Huang McBeath
    • 2
  • Zhong-Kai Zhang
    • 1
  1. 1.Yunnan Provincial Key Laboratory of Agri-Biotechnology, Institute of Biotechnology and Genetic ResourcesYunnan Academy of Agricultural SciencesKunmingPeople’s Republic of China
  2. 2.Plant Pathology and Biotechnology LaboratoryUniversity of Alaska FairbanksFairbanksUSA

Personalised recommendations