Archives of Virology

, Volume 153, Issue 5, pp 831–837 | Cite as

Chitosan as an adjuvant for parenterally administered inactivated influenza vaccines

Original Article


The addition of 0.5% of a chitosan derivative to inactivated influenza vaccines injected parenterally resulted in a four or six to tenfold increase in antibody titres after a single-dose or two-dose intramuscular immunization of mice, respectively, in comparison with antibody titres after immunization without chitosan. Chitosan-adjuvanted vaccines enhanced antibody titers against drift variants of A- and B-type human influenza viruses four to six times compared with the vaccines without chitosan. Inactivated avian influenza virus A/H5N2 admixed with chitosan, when administered to mice challenged afterwards with the same virus, showed higher immunogenicity and protective efficacy compared with the antigen without chitosan.



The authors are deeply grateful to Dr. Smirnov, who kindly provided us with the mouse-adapted avian influenza virus A/H5N2 strain that was needed for our investigations.


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Y. Ghendon
    • 1
  • S. Markushin
    • 1
  • G. Krivtsov
    • 1
  • I. Akopova
    • 1
  1. 1.Mechnikov Research Institute for Vaccines and SeraMoscowRussia

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