Sequences of the smallest double-stranded RNAs associated with cherry chlorotic rusty spot and Amasya cherry diseases
Cherry chlorotic rusty spot (CCRS) and Amasya cherry disease (ACD) are two disorders that affect cherry in Italy  and Turkey . Both diseases have similar symptoms and are associated with a complex pattern of double-stranded RNAs (dsRNAs), absent from healthy-looking material . The dsRNAs were presumed to have a viral origin, and their characterization showed that: (1) four comprise the genomic components of a member of a new tentative species of the genus Chrysovirus , (2) two comprise the genomic components of a member of a new tentative species of the genus Partitivirus , and (3) another four display their highest similarity with members of the family Totiviridae . Because members of these three taxa typically infect fungi, and because microscopy observations have identified a fungus-like organism in the affected leaf tissues , these data support a fungal etiology for CCRS and ACD diseases. In addition to the ten large dsRNAs, PAGE analysis of...
KeywordsTerminal Region Penicillium Chrysogenum Genomic Component Extensive Identity EMBL Nucleotide Sequence Database
Work in the laboratories of R.F. and A.R. was partially supported by the Generalidad Valenciana (ACOMP07/268) of Spain and the Ministero delle Politiche Agricole e Forestali of Italy. L.C. received fellowships from the Marie Curie Program (EU) and from the Università Federico II (Napoli, Italy), and Z.K. a bursary from the Faculty of Natural Sciences, Imperial College (London, UK).
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