Sequences of the smallest double-stranded RNAs associated with cherry chlorotic rusty spot and Amasya cherry diseases
- 111 Downloads
Cherry chlorotic rusty spot (CCRS) and Amasya cherry disease (ACD) are two disorders that affect cherry in Italy  and Turkey . Both diseases have similar symptoms and are associated with a complex pattern of double-stranded RNAs (dsRNAs), absent from healthy-looking material . The dsRNAs were presumed to have a viral origin, and their characterization showed that: (1) four comprise the genomic components of a member of a new tentative species of the genus Chrysovirus , (2) two comprise the genomic components of a member of a new tentative species of the genus Partitivirus , and (3) another four display their highest similarity with members of the family Totiviridae . Because members of these three taxa typically infect fungi, and because microscopy observations have identified a fungus-like organism in the affected leaf tissues , these data support a fungal etiology for CCRS and ACD diseases. In addition to the ten large dsRNAs, PAGE analysis of...
KeywordsTerminal Region Penicillium Chrysogenum Genomic Component Extensive Identity EMBL Nucleotide Sequence Database
Work in the laboratories of R.F. and A.R. was partially supported by the Generalidad Valenciana (ACOMP07/268) of Spain and the Ministero delle Politiche Agricole e Forestali of Italy. L.C. received fellowships from the Marie Curie Program (EU) and from the Università Federico II (Napoli, Italy), and Z.K. a bursary from the Faculty of Natural Sciences, Imperial College (London, UK).
- 2.Blodgett EC, Ismen FH, Gomec B, Kalay K, Altinyay N, Wagnon HK (1970) La maladie d’Amasya du cerisier en Turquie. FAO Plant Prot Bull 18:49–52Google Scholar
- 3.Covelli L, Coutts RHA, Di Serio F, Citir A, Açikgöz S, Hernández C, Ragozzino A, Flores R (2004) Cherry chlorotic rusty spot and Amasya cherry diseases are associated with a complex pattern of mycoviral-like double-stranded RNAs. I. Characterization of a new species in the genus Chrysovirus. J Gen Virol 85:3389–3397PubMedCrossRefGoogle Scholar
- 4.Coutts RHA, Covelli L, Di Serio F, Citir A, Açıkgöz S, Hernández C, Ragozzino A, Flores R (2004) Cherry chlorotic rusty spot and Amasya cherry diseases are associated with a complex pattern of mycoviral-like double-stranded RNAs. II. Characterization of a new species in the genus Partitivirus. J Gen Virol 85:3399–3403PubMedCrossRefGoogle Scholar
- 5.Kozlakidis Z, Covelli L, Di Serio F, Citir A, Açikgöz S, Hernández C, Ragozzino A, Flores R, Coutts RHA (2006) Molecular characterization of the largest mycoviral-like double-stranded RNAs associated with Amasya cherry disease, a disease of presumed fungal aetiology. J Gen Virol 87:3113–3117PubMedCrossRefGoogle Scholar
- 6.Alioto D, Zaccaria F, Covelli L, Ragozzino A, Di Serio F, Milne RG (2003) Light and electron microscope observations on chlorotic rusty spot, a disorder of cherry in Italy. J Plant Pathol 85:215–218Google Scholar
- 8.Ghabrial SA, Soldevila AI, Havens WM (2002) Molecular genetics of the viruses infecting the plant pathogenic fungus Helminthosporium victoriae. In: Tavantzis S (ed) Molecular biology of RNA: concepts and applications in agriculture, forestry and medicine. CRC Press, Boca Raton, pp 213–236Google Scholar
- 10.Ghabrial SA, Liang D, Castón JR (2005) Chrysoviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Eighth report of the international committee on taxonomy of viruses. Elsevier/Academic Press, London, pp 591–595Google Scholar