Archives of Virology

, 153:283 | Cite as

An insufficient anti-inflammatory cytokine response in mouse brain is associated with increased tissue pathology and viral load during Japanese encephalitis virus infection

  • V. Saxena
  • A. Mathur
  • N. Krishnani
  • T. N. DholeEmail author


Infection of the central nervous system with Japanese encephalitis virus (JEV) results in fatal encephalitis in humans. No reports exist describing the sequence of pathological changes and their correlation to the immune response in the brain following infection with JEV. In this report, we analyzed inducible nitric oxide synthase (iNOS) mRNA, proinflammatory (IFN-γ, TNF-α) and anti-inflammatory (IL-4, IL-10) cytokine expression, viral load, and the correlation of these factors with the major histopathological changes in brain of JEV challenged mice at different time points during infection. We report for the first time that in JE, there is a progressive decline in the level of IL-4. The extent of progressive decrease in IL-4 and IL-10 level following viral infection is inversely correlated to the increased level of proinflammatory cytokines and histopathological changes with negative consequences following viral infection. In contrast, proinflammatory mediators like IFN-γ and TNF-α were significantly upregulated (P < 0.05). A negative correlation between IFN-γ and iNOS indicates their independent actions during JEV infection. To conclude, an insufficient anti-inflammatory cytokine response indicated by IL-4 and IL-10 in the brain is associated with increased tissue pathology and viral load, which regulates inflammatory responses driven by IFN-γ in concert with TNF-α to cause brain tissue damage.


Encephalitis West Nile Virus Infected Mouse Japanese Encephalitis Virus Japanese Encephalitis 
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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • V. Saxena
    • 1
  • A. Mathur
    • 2
  • N. Krishnani
    • 3
  • T. N. Dhole
    • 1
    Email author
  1. 1.Department of MicrobiologySanjay Gandhi Postgraduate Institute of Medical SciencesLucknowIndia
  2. 2.Department of MicrobiologySaraswati Medical and Dental CollegeLucknowIndia
  3. 3.Department of PathologySanjay Gandhi Postgraduate Institute of Medical SciencesLucknowIndia

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