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Archives of Virology

, Volume 153, Issue 2, pp 351–356 | Cite as

Characterization of equine arteritis virus particles and demonstration of their hemolytic activity

  • M. Veit
  • A. Kabatek
  • C. Tielesch
  • A. Hermann
Brief Report

Summary

Equine arteritis virus (EAV), a member of the newly established family Arteriviridae, is a small, positive-stranded RNA virus. It carries two protein complexes in its envelope, gp5/M and the recently described gp2b/gp3/gp4 complex. We report here on several basic features of EAV replication in cell culture and on the protein composition of virus particles. We have also characterized gp2b, gp3, and gp4 expressed using a baculovirus system in insect cells. Finally, we provide evidence that EAV possess hemagglutinating and hemolytic activity. The hemolysis assay might be useful for determining which of the surface proteins carries the receptor-binding and membrane fusion activity of EAV.

Keywords

Virus Particle Newcastle Disease Virus Hemolytic Activity Cell Culture Supernatant Virus Preparation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of Immunology and Molecular Biology, Veterinary FacultyFree University BerlinBerlinGermany
  2. 2.Institute of BiologyHumboldt-UniversityBerlinGermany

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