Archives of Virology

, Volume 152, Issue 12, pp 2237–2247 | Cite as

Natural M-segment reassortment in Potosi and Main Drain viruses: implications for the evolution of orthobunyaviruses

  • T. Briese
  • V. Kapoor
  • W. I. Lipkin


Recently, we identified Batai virus as the M-segment reassortment partner of Ngari virus. Extension of genetic analyses to other orthobunyaviruses related to the Bunyamwera serogroup indicates additional natural genome reassortments. Whereas the relative phylogenetic positions of all three genome segment sequences were similar for Northway and Kairi viruses, the relative positions of Potosi and Main Drain virus M-segment sequences diverged from those of their S- and L-segments. Our findings indicate M-segment reassortment in Potosi and Main Drain viruses and demonstrate natural genome reassortment as a driving force in the evolution of viruses of the Bunyamwera serogroup.


Reassortant Virus Albopictus Mosquito Cache Valley Virus Jamestown Canyon Virus Batai Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Aitken, TH, Spence, L 1963Virus transmission studies with Trinidadian mosquitoes. III. Cache Valley virusWest Indian Med J12128132PubMedGoogle Scholar
  2. Anderson, CR, Aitken, TH, Spence, LP, Downs, WG 1960Kairi virus, a new virus from Trinidadian forest mosquitoesAm J Trop Med Hyg97072PubMedGoogle Scholar
  3. Armstrong, PM, Andreadis, TG, Anderson, JF, Main, AJ 2005Isolations of Potosi virus from mosquitoes (Diptera: Culicidae) collected in ConnecticutJ Med Entomol42875881PubMedCrossRefGoogle Scholar
  4. Beaty, BJ, Sundin, DR, Chandler, LJ, Bishop, DH 1985Evolution of bunyaviruses by genome reassortment in dually infected mosquitoes (Aedes triseriatus)Science230548550PubMedCrossRefGoogle Scholar
  5. Blackmore, CG, Grimstad, PR 1998Cache Valley and Potosi viruses (Bunyaviridae) in white-tailed deer (Odocoileus virginianus): experimental infections and antibody prevalence in natural populationsAm J Trop Med Hyg59704709PubMedGoogle Scholar
  6. Borucki, MK, Chandler, LJ, Parker, BM, Blair, CD, Beaty, BJ 1999Bunyavirus superinfection and segment reassortment in transovarially infected mosquitoesJ Gen Virol8031733179PubMedGoogle Scholar
  7. Bridgen, A, Weber, F, Fazakerley, JK, Elliott, RM 2001Bunyamwera bunyavirus nonstructural protein NSs is a nonessential gene product that contributes to viral pathogenesisProc Natl Acad Sci USA98664669PubMedCrossRefGoogle Scholar
  8. Briese, T, Rambaut, A, Lipkin, WI 2004Analysis of the medium (M) segment sequence of Guaroa virus and its comparison to other orthobunyavirusesJ Gen Virol8530713077PubMedCrossRefGoogle Scholar
  9. Briese, T, Palacios, G, Kokoris, M, Jabado, O, Liu, Z, Renwick, N, Kapoor, V, Casas, I, Pozo, F, Limberger, R, Perez-Brena, P, Ju, J, Lipkin, WI 2005Diagnostic system for rapid and sensitive differential detection of pathogensEmerg Infect Dis11310313PubMedGoogle Scholar
  10. Briese, T, Bird, B, Kapoor, V, Nichol, ST, Lipkin, WI 2006Batai and Ngari viruses: M segment reassortment and association with severe febrile disease outbreaks in East AfricaJ Virol8056275630PubMedCrossRefGoogle Scholar
  11. Calisher, CH, Lindsey, HS, Ritter, DG, Sommerman, KM 1974Northway virus: a new Bunyamwera group arbovirus from AlaskaCan J Microbiol20219223PubMedCrossRefGoogle Scholar
  12. Calisher, CH, Francy, DB, Smith, GC, Muth, DJ, Lazuick, JS, Karabatsos, N, Jakob, WL, McLean, RG 1986Distribution of Bunyamwera serogroup viruses in North America, 1956–1984Am J Trop Med Hyg35429443PubMedGoogle Scholar
  13. Calisher, CH, Oro, JG, Lord, RD, Sabattini, MS, Karabatsos, N 1988Kairi virus identified from a febrile horse in ArgentinaAm J Trop Med Hyg39519521PubMedGoogle Scholar
  14. Calisher, CH, Sabattini, MS, Monath, TP, Wolff, KL 1988Cross-neutralization tests among Cache Valley virus isolates revealing the existence of multiple subtypesAm J Trop Med Hyg39202205PubMedGoogle Scholar
  15. Camara, A, Contigiani, MS, Medeot, SI 1990Concomitant activity of 2 bunyaviruses in horses in ArgentinaRev Argent Microbiol2298101PubMedGoogle Scholar
  16. Campbell, GL, Hardy, JL, Eldridge, BF, Reeves, WC 1991Isolation of Northway serotype and other Bunyamwera serogroup bunyaviruses from California and Oregon mosquitoes, 1969–1985Am J Trop Med Hyg44581588PubMedGoogle Scholar
  17. Casals, J, Whitman, L 1960A new antigenic group of arthropod-borne viruses: the Bunyamwera groupAm J Trop Med Hyg97377PubMedGoogle Scholar
  18. Catalogue of arthropod-borne viruses of the world (1970) No. 219; Main Drain (MD); strain: BFS 5015. Am J Trop Med Hyg 19 [Suppl]: 1109–1110Google Scholar
  19. Causey, OR, Causey, CE, Maroja, OM, Macedo, DG 1961The isolation of arthropod-borne viruses, including members of two hitherto undescribed serological groups, in the Amazon region of BrazilAm J Trop Med Hyg10227249PubMedGoogle Scholar
  20. Claros, MG, von Heijne, G 1994TopPred II: an improved software for membrane protein structure predictionsComput Appl Biosci10685686PubMedGoogle Scholar
  21. Downs, WG, Spence, L, Aitken, TH, Whitman, L 1961Cache Valley virus, isolated from a Trinidadian mosquito, Aedes scapularis West Indian Med J101315PubMedGoogle Scholar
  22. Dunn, EF, Pritlove, DC, Elliott, RM 1994The S RNA genome segments of Batai, Cache Valley, Guaroa, Kairi, Lumbo, Main Drain and Northway bunyaviruses: sequence determination and analysisJ Gen Virol75597608PubMedCrossRefGoogle Scholar
  23. Elliott, RM eds. 1996The BunyaviridaePlenum PressNew York, USAGoogle Scholar
  24. Fazakerley, JK, Gonzalez-Scarano, F, Strickler, J, Dietzschold, B, Karush, F, Nathanson, N 1988Organization of the middle RNA segment of snowshoe hare bunyavirusVirology167422432PubMedGoogle Scholar
  25. Francy, DB, Karabatsos, N, Wesson, DM, Moore, CG,Jr, Lazuick, JS, Niebylski, ML, Tsai, TF, Craig, GB,Jr 1990A new arbovirus from Aedes albopictus, an Asian mosquito established in the United StatesScience25017381740PubMedCrossRefGoogle Scholar
  26. Gentsch, JR, Rozhon, EJ, Klimas, RA, El Said, LH, Shope, RE, Bishop, DH 1980Evidence from recombinant bunyavirus studies that the M RNA gene products elicit neutralizing antibodiesVirology102190204PubMedCrossRefGoogle Scholar
  27. Gerrard, SR, Li, L, Barrett, AD, Nichol, ST 2004Ngari virus is a Bunyamwera virus reassortant that can be associated with large outbreaks of hemorrhagic fever in AfricaJ Virol7889228926PubMedCrossRefGoogle Scholar
  28. Holden, P, Hess, AD 1959Cache Valley virus, a previously undescribed mosquito-borne agentScience13011871188PubMedCrossRefGoogle Scholar
  29. Iroegbu, CU, Pringle, CR 1981Genetic interactions among viruses of the Bunyamwera complexJ Virol37383394PubMedGoogle Scholar
  30. Jabado, O, Palacios, G, Kapoor, V, Hui, J, Renwick, N, Zhai, J, Briese, T, Lipkin, WI 2007Greene SCPrimer: a rapid comprehensive tool for designing degenerate primers from multiple sequence alignmentsNucleic Acids Res3466056611CrossRefGoogle Scholar
  31. Käll, L, Krogh, A, Sonnhammer, EL 2004A combined transmembrane topology and signal peptide prediction methodJ Mol Biol33810271036PubMedCrossRefGoogle Scholar
  32. Klimas, RA, Thompson, WH, Calisher, CH, Clark, GG, Grimstad, PR, Bishop, DH 1981Genotypic varieties of La Crosse virus isolated from different geographic regions of the continental United States and evidence for a naturally occurring intertypic recombinant La Crosse virusAm J Epidemiol114112131PubMedGoogle Scholar
  33. Kramer, WL, Jones, RH, Holbrook, FR, Walton, TE, Calisher, CH 1990Isolation of arboviruses from Culicoides midges (Diptera: Ceratopogonidae) in Colorado during an epizootic of vesicular stomatitis New JerseyJ Med Entomol27487493PubMedGoogle Scholar
  34. Krogh, A, Larsson, B, von Heijne, G, Sonnhammer, EL 2001Predicting transmembrane protein topology with a hidden Markov model: application to complete genomesJ Mol Biol305567580PubMedCrossRefGoogle Scholar
  35. Kumar, S, Tamura, K, Nei, M 2004MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignmentBrief Bioinform5150163PubMedCrossRefGoogle Scholar
  36. Lamson, D, Renwick, N, Kapoor, V, Liu, Z, Palacios, G, Ju, J, Dean, A, St George, K, Briese, T, Lipkin, WI 2006MassTag polymerase-chain-reaction detection of respiratory pathogens, including a new rhinovirus genotype, that caused influenza-like illness in New York state during 2004–2005J Infect Dis19413981402PubMedCrossRefGoogle Scholar
  37. McLean, RG, Kirk, LJ, Shriner, RB, Cook, PD, Myers, EE, Gill, JS, Campos, EG 1996The role of deer as a possible reservoir host of Potosi virus, a newly recognized arbovirus in the United StatesJ Wildl Dis32444452PubMedGoogle Scholar
  38. Mitchell, CJ, Smith, GC, Miller, BR 1990Vector competence of Aedes albopictus for a newly recognized bunyavirus from mosquitoes collected in Potosi, MissouriJ Am Mosq Control Assoc6523527PubMedGoogle Scholar
  39. Mitchell, CJ, Haramis, LD, Karabatsos, N, Smith, GC, Starwalt, VJ 1998Isolation of La Crosse, Cache Valley, and Potosi viruses from Aedes mosquitoes (Diptera: Culicidae) collected at used-tire sites in Illinois during 1994–1995J Med Entomol35573577PubMedGoogle Scholar
  40. Nagayama, JN, Komar, N, Levine, JF, Apperson, CS 2001Bunyavirus infections in North Carolina white-tailed deer (Odocoileus virginianus)Vector Borne Zoonotic Dis1169171PubMedCrossRefGoogle Scholar
  41. Ngo, KA, Maffei, JG, Dupuis, AP,2nd, Kauffman, EB, Backenson, PB, Kramer, LD 2006Isolation of Bunyamwera serogroup viruses (Bunyaviridae, Orthobunyavirus) in New York stateJ Med Entomol4310041009PubMedCrossRefGoogle Scholar
  42. Nichol, ST 2001BunyavirusesKnipe, DMHowley, PM eds. Fields virologyLippincott, Williams & WilkinsPhiladelphia16031633Google Scholar
  43. Nielsen, H, Engelbrecht, J, Brunak, S, von Heijne, G 1997Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sitesProtein Eng1016PubMedCrossRefGoogle Scholar
  44. Nielsen H, Krogh A (1998) Prediction of signal peptides and signal anchors by a hidden Markov model. Proceedings of the Sixth International Conference on Intelligent Systems for Molecular Biology (ISMB6), Menlo Park, California. AAAI Press, pp 122–130Google Scholar
  45. Palacios, G, Quan, P-L, Jabado, OJ, Conlan, S, Hirschberg, DL, Liu, Y, Zhai, J, Renwick, N, Hui, J, Hegyi, H, Grolla, A, Strong, JE, Towner, JS, Geisbert, TW, Jahrling, PB, Büchen-Osmond, C, Ellerbrok, H, Sanchez-Seco, MP, Lussier, Y, Formenty, P, Nichol, ST, Feldmann, H, Briese, T, Lipkin, WI 2006Panmicrobial oligonucleotide array for diagnosis of infectious diseasesEmerg Infect Dis137381CrossRefGoogle Scholar
  46. Plassmeyer, ML, Soldan, SS, Stachelek, KM, Roth, SM, Martin-Garcia, J, Gonzalez-Scarano, F 2007Mutagenesis of the La Crosse Virus glycoprotein supports a role for Gc (1066–1087) as the fusion peptideVirology358273282PubMedCrossRefGoogle Scholar
  47. Pollitt, E, Zhao, J, Muscat, P, Elliott, RM 2006Characterization of Maguari orthobunyavirus mutants suggests the nonstructural protein NSm is not essential for growth in tissue cultureVirology348224232PubMedCrossRefGoogle Scholar
  48. Pringle, CR, Lees, JF, Clark, W, Elliott, RM 1984Genome subunit reassortment among bunyaviruses analysed by dot hybridization using molecularly cloned complementary DNA probesVirology135244256PubMedCrossRefGoogle Scholar
  49. Saiki, RK, Scharf, S, Faloona, F, Mullis, KB, Horn, GT, Erlich, HA, Arnheim, N 1985Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemiaScience23013501354PubMedCrossRefGoogle Scholar
  50. Sanger, F, Nicklen, S, Coulson, AR 1977DNA sequencing with chain-terminating inhibitorsProc Natl Acad Sci USA7454635467PubMedCrossRefGoogle Scholar
  51. Schmaljohn, CS, Hooper, JW 2001Bunyaviridae: the viruses and their replicationKnipe, DMHowley, PM eds. Fields virologyLippincott, Williams & WilkinsPhiladelphia15811602Google Scholar
  52. Shi, X, Kohl, A, Leonard, VH, Li, P, McLees, A, Elliott, RM 2006Requirement of the N-terminal region of orthobunyavirus nonstructural protein NSm for virus assembly and morphogenesisJ Virol8080898099PubMedCrossRefGoogle Scholar
  53. Weber, F, Bridgen, A, Fazakerley, JK, Streitenfeld, H, Kessler, N, Randall, RE, Elliott, RM 2002Bunyamwera bunyavirus nonstructural protein NSs counteracts the induction of alpha/beta interferonJ Virol7679497955PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • T. Briese
    • 1
  • V. Kapoor
    • 1
  • W. I. Lipkin
    • 1
  1. 1.Center for Infection and Immunity, Mailman School of Public HealthColumbia UniversityNew YorkUSA

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