Archives of Virology

, Volume 152, Issue 9, pp 1751–1756 | Cite as

Bluetongue virus induces apoptosis in cultured mammalian cells by both caspase-dependent extrinsic and intrinsic apoptotic pathways

  • V. K. Nagaleekar
  • A. K. Tiwari
  • R. S. Kataria
  • M. V. Bais
  • P. V. Ravindra
  • S. Kumar
Brief Report

Summary

Bluetongue virus (BTV) causes haemorrhagic disease in sheep and induces death in cultured mammalian cells. In the present study, BTV-induced apoptotic pathways in Vero cells were elucidated. Cells infected with BTV at 0.1 m.o.i underwent DNA fragmentation and membrane blebbing within 48 h postinfection. BTV-induced apoptosis was blocked by the pan-caspase inhibitor, z-VAD-FMK. Immuno-blotting using anti-caspase-8 and -9 antibodies detected the activation of the respective caspases. Flow cytometry analyses following 3, 3′ dihexyloxacarbocyanine iodide staining revealed the loss of mitochondrial membrane potential. Our study confirms the involvement of both caspase-dependent extrinsic and intrinsic pathways of apoptosis in BTV-infected cells.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arends, MJ, Morris, RG, Willey, AJ 1990Apoptosis: the role of the endonucleaseAm J Pathol136593608PubMedGoogle Scholar
  2. Arends, M, Wellie, A 1991Apoptosis: mechanism and roles in pathologyInt Rev Exp Pathol32223254PubMedGoogle Scholar
  3. Chang, CJ, Shih, WL, Yu, FL, Liao, MH, Liu, HJ 2004Apoptosis induced by bovine ephemeral fever virusJ Virol Meth122165170CrossRefGoogle Scholar
  4. Chinnaiyan, AM, Chaudhary, D, O’Rourke, K, Koonin, EV, Dixit, VM 1997Role of CED-4 in the activation of CED-3Nature388728729PubMedCrossRefGoogle Scholar
  5. DeMaula, CD, Jutila, MA, Wilson, DW, MacLachlan, NJ 2001Infection kinetics, prostacycline release and cytokine mediated modulation of the mechanism of cell death during bluetongue virus infection of cultured ovine and pulmonary artery and lung microvascular endothelial cellsJ Gen Virol82787794PubMedGoogle Scholar
  6. DeMaula, CD, Leutenegger, CM, Jutila, MA, MacLachlan, NJ 2002Bluetongue virus induced activation of primary bovine lung microvascular endothelial cellsVet Immunol Immunopathol86147157PubMedCrossRefGoogle Scholar
  7. Dong, X, Kukkonen, S, Vapalahti, O, Plysnin, A, Lankinen, H, Vaheri, A 2004Tula hantavirus infection of Vero cells induces apoptosis involving caspase-8 activationJ Gen Virol8532613268CrossRefGoogle Scholar
  8. Faber, M, Pulmanausahakul, R, Hodawadekar, SS, Spitsin, S, Mcgetigan, JP, Schnell, MJ, Dietzschold, B 2002Overexpression of the rabies virus glycoprotein results in enhancement of apoptosis and antiviral immune responseJ Virol7633743381PubMedCrossRefGoogle Scholar
  9. Hay, S, Kannourakis, G 2000A time to kill: viral manipulation of cell death programJ Gen Virol8315471564Google Scholar
  10. Herrman, M, Lorenz, HM, Voll, R, Grunke, M, Woith, W, Kalden, JR 1994A rapid and simple method for the isolation of apoptotic DNA fragmentsNucl Acids Res2255065507CrossRefGoogle Scholar
  11. Houge, G, Doskeland, SO 1996Divergence towards the end? Cleavage of the divergent domains of ribosomal RNA in apoptosisExperientia52963967PubMedCrossRefGoogle Scholar
  12. Hu, S, Vincenz, C, Ni, J, Gentz, R, Dixit, VM 1997I-Flice, a novel inhibitor of tumor necrosis factor receptor-1 and CD95-induced apoptosisJ Biol Chem2721725517257PubMedCrossRefGoogle Scholar
  13. Kerr, JFR, Wyllie, AH, Currie, AR 1972Apoptosis: a basic biological phenomenon with wide ranging implications in tissue kineticsBr J Cancer26239257PubMedGoogle Scholar
  14. Mesner, PW,Jr, Bible, KC, Martins, LM, Kottke, TJ, Srinivasula, SM, Svingen, PA, Chilicote, TJ, Basi, GS, Tung, JS, Krajewski, S, Reed, JC, Alnemri, ES, Earnshaw, WC, Kaufmann, SH 1999Characterization of caspase processing and activation in HL-60 cell cytosol under cell-free conditionsJ Biol Chem2742263522645PubMedCrossRefGoogle Scholar
  15. Mortola, E, Noad, R, Roy, P 2004Blutongue virus outer capsid proteins are sufficient to trigger apoptosis in mammalian cellsJ Virol7828752883PubMedCrossRefGoogle Scholar
  16. Nicholson, DW, Thornberry, NA 1997Caspases: killer proteasesTrends Biochem Sci22299306PubMedCrossRefGoogle Scholar
  17. Roulston, A, Marcellus, RC, Branton, PE 1999Viruses and apoptosisAnn Rev Microbiol53577628CrossRefGoogle Scholar
  18. Shih, WL, Hsu, WH, Lee, LH, Liao, MH, Liu, HJ 2004Avian reovirus σC protein induces apoptosis in cultured cellsVirol3216574CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • V. K. Nagaleekar
    • 1
  • A. K. Tiwari
    • 3
  • R. S. Kataria
    • 3
  • M. V. Bais
    • 2
  • P. V. Ravindra
    • 3
  • S. Kumar
    • 3
  1. 1.Given D305BurlingtonUSA
  2. 2.Boston University Medical CampusBostonUSA
  3. 3.Division of Veterinary BiotechnologyIndian Veterinary Research InstituteIzatnagarIndia

Personalised recommendations