Archives of Virology

, Volume 152, Issue 8, pp 1515–1521 | Cite as

Molecular characterization of the surface glycoprotein genes of an H5N1 influenza virus isolated from a human in Guangdong, China

  • J.-J. Zhou
  • J. Fu
  • D.-Y. Fang
  • H.-J. Yan
  • J. Tian
  • J.-M. Zhou
  • J.-P. Tao
  • Y. Liang
  • L.-F. Jiang
Brief Report

Summary

In March 2006, a human H5N1-infected case was found in Guangdong province, China. Here, we molecularly characterized the hemagglutinin (HA) and neuraminidase (NA) genes of the A/China/GD01/06 (GD01) strain causing the infection. The phylogenetic analyses suggested that the HA and NA genes of GD01 and recent human H5N1 viruses from different provinces of China were probably derived from a common ancestor and the H5N1 human infection was acquired directly from affected poultry. At the cleavage site of HA, GD01 contained multiple basic amino acids, a feature characteristic of highly pathogenic avian influenza A viruses. The virus possessed Gln222, Gly224, Ser223, Asn182, Gln192 residues adjacent to the receptor-binding site, preferential for recognizing SAα2, 3Gal. In addition, the GD01 NA amino acid sequence possessed Asn344 and Phe466, which might be related to the low-pH stability of the sialidase activity and gastrointestinal symptoms of the patient.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • J.-J. Zhou
    • 1
  • J. Fu
    • 1
  • D.-Y. Fang
    • 1
  • H.-J. Yan
    • 1
  • J. Tian
    • 1
  • J.-M. Zhou
    • 1
  • J.-P. Tao
    • 1
  • Y. Liang
    • 1
  • L.-F. Jiang
    • 1
  1. 1.Department of Microbiology, Zhongshan School of MedicineSun Yat-sen UniversityGuangzhouChina

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