Archives of Virology

, Volume 152, Issue 8, pp 1541–1546 | Cite as

Susceptibility of cyprinid cultured cells to cyprinid herpesvirus 3

  • M. Davidovich
  • A. Dishon
  • M. Ilouze
  • M. Kotler
Brief Report


Cyprinid herpesvirus 3 is a highly contagious and lethal virus that affects ornamental koi and common carp worldwide. However, it is not yet known whether other cyprinids are infected and/or harbor the virus. Here, we report that cultured cells derived from common carp, koi, silver carp and goldfish allow CyHV-3 propagation, while cyprinid cells derived from fathead minnow and non-cyprinid cells derived from the channel catfish ovary are resistant to CyHV-3 infection. Interestingly, the epithelioma papulosum Cyprini cells derived from Cyprinus carpio are restrictive to the virus. These results indicate that CyHV-3 is not restricted to common carp and koi, but other cyprinids are also vulnerable to the virus.


Common Carp Grass Carp Silver Carp Epithelioma Papulosum Cyprini Epithelioma Papulosum Cyprini Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aoki T, Hirono I, Kurokawa K, Fukuda H, Nahary R, Eldar A, Davison AJ, Waltzek TB, Bercovier H, Hedrick RP (2007) Genome sequences of three koi herpesvirus isolates representing the expanding distribution of an emerging disease threatening koi and common carp worldwide. J Virol [Epub ahead of print]Google Scholar
  2. Dishon, A, Perelberg, A, Bishara-Shieban, J, Ilouze, M, Davidovich, M, Werker, S, Kotler, M 2005Detection of carp interstitial nephritis and gill necrosis virus in fish droppingsAppl Environ Microbiol7172857291PubMedCrossRefGoogle Scholar
  3. Dishon, A, Davidovich, M, Ilouze, M, Kotler, M 2007Persistence of cyprinid herpes virus 3 (CyHV-3) in infected cultured carp cellsJ Virol8148284836PubMedCrossRefGoogle Scholar
  4. Gilad, O, Yun, S, Adkison, MA, Way, K, Willits, NH, Bercovier, H, Hedrick, RP 2003Molecular comparison of isolates of an emerging fish pathogen, koi herpesvirus, and the effect of water temperature on mortality of experimentally infected koiJ Gen Virol8426612667PubMedCrossRefGoogle Scholar
  5. Gilad, O, Yun, S, Zagmutt-Vergara, FJ, Leutenegger, CM, Bercovier, H, Hedrick, RP 2004Concentrations of a Koi herpesvirus (KHV) in tissues of experimentally infected Cyprinus carpio koi as assessed by real-time TaqMan PCRDis Aquat Org60179187PubMedGoogle Scholar
  6. Gray, WL, Mullis, L, LaPatra, SE, Groff, M, Goodwin, A 2002Detection of koi herpesvirus DNA in tissues of infected fishJ Fish Dis25171178CrossRefGoogle Scholar
  7. Grimmett, SG, Warg, JV, Getchell, RG, Johnson, DJ, Bowser, PR 2006An unusual koi herpesvirus associated with a mortality event of common carp Cyprinus carpio in New York State, USAJ Wildl Dis42658662PubMedGoogle Scholar
  8. Haramoto, E, Kitajima, M, Katayama, H, Ohgaki, S 2007Detection of koi herpesvirus DNA in river water in JapanJ Fish Dis305961PubMedCrossRefGoogle Scholar
  9. Hasegawa, S, Somamoto, T, Nakayasu, C, Nakanishi, T, Okamoto, N 1997A cell line (CFK) from fin of isogeneic ginbuna crusian carpFish Pathol32127128Google Scholar
  10. Hedrick, RP, Gilad, O, Yun, S, Spangenberg, J, Marty, G, Nordhausen, R, Kebus, M, Bercovier, H, Eldar, A 2000A herpesvirus associated with mass mortality of juvenile and adult koi, a strain of common carpJ Aquat Animal Health124455CrossRefGoogle Scholar
  11. Hedrick, RP, Waltzek, TB, McDowell, TS 2006Susceptibility of Koi Carp, Common Carp, Goldfish, and Goldfish × Common Carp Hybrids to Cyprinid Herpesvirus-2 and Herpersvirus-3J Aquat Animal Health182634CrossRefGoogle Scholar
  12. Hutoran, M, Ronen, A, Perelberg, A, Ilouze, M, Dishon, A, Bejerano, I, Chen, N, Kotler, M 2005Description of an as yet unclassified DNA virus from diseased Cyprinus carpio speciesJ Virol7919831991PubMedCrossRefGoogle Scholar
  13. Ilouze, M, Dishon, A, Kahan, T, Kotler, M 2006Cyprinid herpes virus-3 (CyHV-3) bears genes of genetically distant large DNA virusesFEBS Lett58044734478PubMedCrossRefGoogle Scholar
  14. Ilouze, M, Dishon, A, Kotler, M 2006Characterization of a novel virus causing a lethal disease in carp and koiMicrobiol Mol Biol Rev70147156PubMedCrossRefGoogle Scholar
  15. Ishioka, T, Yoshizumi, M, Izumi, S, Suzuki, K, Suzuki, H, Kozawa, K, Arai, M, Nobusawa, K, Morita, Y, Kato, M, Hoshino, T, Iida, T, Kosuge, K, Kimura, H 2005Detection and sequence analysis of DNA polymerase and major envelope protein genes in koi herpesviruses derived from Cyprinus carpio in Gunma prefecture, JapanVet Microbiol1102733PubMedCrossRefGoogle Scholar
  16. Kondo, H, Watabe, S 2004Temperature-dependent enhancement of cell proliferation and mRNA expression for type I collagen and HSP70 in primary cultured goldfish cellsComp Biochem Physiol A Mol Integr Physiol138221228PubMedCrossRefGoogle Scholar
  17. McGeoch, DJ, Rixon, FJ, Davison, AJ 2006Topics in herpesvirus genomics and evolutionVirus Res11790104PubMedCrossRefGoogle Scholar
  18. Neukirch, M, Bottcher, K, Bunnajirakul, S 1999Isolation of a virus from koi with altered gillsBull Eur Ass Fish Pathol19221224Google Scholar
  19. Perelberg, A, Smirnov, M, Hutoran, M, Diamant, A, Bejerano, I, Kotler, M 2003Epidemiological description of a new viral disease afflicting cultured Cyprinus carpio in IsraelIsr J Aquacult Bamidgeh55512Google Scholar
  20. Perelberg, A, Ronen, A, Hutoran, M, Smith, Y, Kotler, M 2005Protection of cultured Cyprinus carpio against a lethal viral disease by an attenuated virus vaccineVaccine2333963403PubMedCrossRefGoogle Scholar
  21. Pikarsky, E, Ronen, A, Abramowitz, J, Levavi-Sivan, B, Hutoran, M, Shapira, Y, Steinitz, M, Perelberg, A, Soffer, D, Kotler, M 2004Pathogenesis of acute viral disease induced in fish by carp interstitial nephritis and gill necrosis virusJ Virol7895449551PubMedCrossRefGoogle Scholar
  22. Ronen, A, Perelberg, A, Abramowitz, J, Hutoran, M, Tinman, S, Bejerano, I, Steinitz, M, Kotler, M 2003Efficient vaccine against the virus causing a lethal disease in cultured Cyprinus carpioVaccine2146774684PubMedCrossRefGoogle Scholar
  23. Ronen, A, Perelberg, A, Hutoran, M, Shapira, Y, Steinitz, M, Levavi-Sivan, B, Pikarsky, E, Kotler, M 2005Prevention of a mortal disease of carps induced by the carp interstitial and gill necrosis virus (CNGV) in IsraelBull Fish Agen Suppl2911Google Scholar
  24. Shapira, Y, Magen, Y, Zak, T, Kotler, M, Hulata, G, Levavi-Sivan, B 2005Differential resistance to koi herpes virus (KHV)/carp nephritis and gill necrosis virus (CNGV) among common carp (Cyprinus carpio) strains and crossbredsAquaculture245111CrossRefGoogle Scholar
  25. St-Hilaire, S, Beevers, N, Way, K, Le Deuff, RM, Martin, P, Joiner, C 2005Reactivation of koi herpesvirus infections in common carp Cyprinus carpioDis Aquat Organ671523PubMedGoogle Scholar
  26. Waltzek, TB, Kelley, GO, Stone, DM, Way, K, Hanson, L, Fukuda, H, Hirono, I, Aoki, T, Davison, AJ, Hedrick, RP 2005Koi herpesvirus represents a third cyprinid herpesvirus (CyHV-3) in the family HerpesviridaeJ Gen Virol8616591667PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • M. Davidovich
    • 1
  • A. Dishon
    • 1
  • M. Ilouze
    • 1
  • M. Kotler
    • 1
  1. 1.Department of PathologyThe Hebrew University-Hadassah Medical SchoolJerusalemIsrael

Personalised recommendations