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Archives of Virology

, Volume 153, Issue 3, pp 571–577 | Cite as

Biological and molecular characterization of tospoviruses in Thailand

  • Pissawan ChiemsombatEmail author
  • Oraprapai Gajanandana
  • Nuchanard Warin
  • Ratchanee Hongprayoon
  • Anjana Bhunchoth
  • Preyapan Pongsapich
Brief Report

Abstract

Twenty-eight isolates of tospoviruses associated with tomato, pepper, cucurbits, peanut, and Physalis plants collected from fields in different regions of Thailand were characterized. On the basis of N gene and protein sequence relationships, three tospoviruses were identified, namely Watermelon silver mottle virus (WSMoV), Capsicum chlorosis virus (CaCV), and Melon yellow spot virus (MYSV). CLUSTAL analysis of selected N protein sequences showed different isolates of CaCV in three distinct clades. Based on necrosis symptoms on tomato and their 93% identity to CaCV isolates in the other two clades, CaCV-TD8, CaCV-AIT and CaCV-KS16-Thailand tomato tospovirus were designated as CaCV-tomato necrosis strain. A phylogenetic tree based on the 413-amino-acid Gc fragment of the CaCV-Pkk isolate supported the existence of three distinct CaCV clades. Vigna unguiculata produced concentric rings useful for discriminating the Thai CaCV peanut isolates from tomato or pepper isolates. By using reverse transcription polymerase chain reaction with species-specific primers, the three tospoviruses could be detected in mixed infections in watermelon and Physalis, as well as in the bodies of thrips vectors, Thrips palmi and Scirtothrips dorsalis, collected from fields.

Keywords

Tomato Spotted Wilt Virus Iris Yellow Spot Virus Thrips Species Melon Yellow Spot Virus Thai Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Dr. Scott Adkins for assistance in preparing and critical reading of the manuscript. We thank Dr. John Thomas for critical reading and comments. The study was supported in part by grants from Thailand Toray Science Foundation, and from BIOTEC, Ministry of Science and Technology. Support was also provided by Center of Excellence on Agricultural Biotechnology, Kasetsart University, Kamphaengsaen Campus, Thailand.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Pissawan Chiemsombat
    • 1
    Email author
  • Oraprapai Gajanandana
    • 2
  • Nuchanard Warin
    • 2
  • Ratchanee Hongprayoon
    • 1
  • Anjana Bhunchoth
    • 2
  • Preyapan Pongsapich
    • 3
  1. 1.Department of Plant Pathology, Faculty of AgricultureKasetsart University, Kamphaengsaen CampusNakhon PathomThailand
  2. 2.Central Laboratory Unit, National Center for Genetic Engineering and Biotechnology, Ministry of Science and TechnologyPathum ThaniThailand
  3. 3.Plant Protection Division, Ministry of Agriculture and CooperativesBangkokThailand

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