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Archives of Virology

, Volume 153, Issue 3, pp 533–539 | Cite as

Infectivity of the cloned components of a begomovirus: DNA beta complex causing chilli leaf curl disease in India

  • B. Chattopadhyay
  • A. K. Singh
  • T. Yadav
  • C. M. Fauquet
  • N. B. Sarin
  • S. ChakrabortyEmail author
Brief Report

Abstract

The full-length genome of a begomovirus and its cognate DNA-β satellite component associated with chilli leaf curl disease (ChLCD), originating from Varanasi, India, were cloned. Sequence analysis revealed that the viral genome (EF190217) is 2,750 bp and the DNA-β satellite (EF190215) is 1,361 bp in length. Agroinoculation with partial tandem repeats of the viral genome along with the satellite induced symptoms typical of ChLCD in chilli and Nicotiana benthamiana. However, symptom expression was delayed and milder when the viral genome was agroinoculated alone in these hosts. Sequence comparisons revealed that the genome had the highest sequence identity (95%) with that of chilli leaf curl virus-PK[PK:Mul:98]. The DNA-β satellite shared maximum sequence identity (88%) with a DNA-β satellite associated with tomato leaf curl disease from Rajasthan (ToLCBDB-[IN:Raj:03]). These results demonstrate that ChLCD is caused by a complex consisting of the monopartite chilli leaf curl virus and a DNA-β satellite component. This is the first experimental demonstration of Koch’s postulates using cloned DNA molecules associated with chilli leaf curl disease.

Keywords

Viral Genome Tomato Leaf Leaf Curling High Nucleotide Sequence Identity Chilli Plant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported by the Department of Science and Technology, Government of India.

Supplementary material

705_2007_17_MOESM1_ESM.doc (36 kb)
Table 1: GenBank accession numbers of selected Begomovirus DNA-A sequences from Asia used in study for analysis (DOC 36.0 kb)
705_2007_17_MOESM2_ESM.doc (33 kb)
Table 2: GenBank accession numbers of selected Begomovirus DNA-β satellite sequences from Asia used in study for analysis (DOC 33.0 kb)

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • B. Chattopadhyay
    • 1
  • A. K. Singh
    • 1
  • T. Yadav
    • 1
  • C. M. Fauquet
    • 2
  • N. B. Sarin
    • 1
  • S. Chakraborty
    • 1
    Email author
  1. 1.Molecular Virology Laboratory, School of Life SciencesJawaharlal Nehru UniversityNew DelhiIndia
  2. 2.Danforth Plant Science CenterSaint LouisUSA

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