Novel sugarcane streak and sugarcane streak Reunion mastreviruses from southern Africa and La Réunion
- 169 Downloads
The sugarcane infecting streak viruses (SISVs) are mastreviruses (Family Geminiviridae) belonging to a group of “African streak viruses” (AfSVs) that includes the economically devastating Maize streak virus (MSV). Although there are three currently described SISV species (Sugarcane streak virus [SSV], Sugarcane streak Egypt virus [SSEV] and Sugarcane streak Réunion virus [SSRV]), only one strain variant has been fully sequenced for each of these species and as a result very little is known about the diversity and evolutionary origins of the SCISVs. Here we present annotated full genome sequences of four new SISV isolates, including a new strain of both SSRV and SSV, and one potentially new SISV species, sampled from wild grasses in La Réunion and Zimbabwe. For the first time, we report the finding of SSRV isolates in Zimbabwe and SSV isolates on the island of La Réunion. Phylogenetic and recombination analyses indicate continent-wide SSRV strain diversity and that our isolate potentially representing a new SISV species is a recombinant.
KeywordsFull Genome Sequence Streak Virus Maize Streak Virus Indian Ocean Island Branch Point Sequence
This research was partially funded by the National Research Foundation (South Africa). AV is supported by the Carnegie Corporation of New York; DNS is supported by PANNAR (Pty) Ltd; DPM is supported by the Harry Oppenheimer Trust and the Sydney Brenner Fellowship; PL is supported by the French Ministère de la Recherche et de l’Enseignement Supérieur; BO is supported by the Rockefeller foundation through the USHEPiA programme; ALM is supported by the Canon Collins Trust for Southern Africa and a University of Cape Town International Scholarship.
- 5.Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
- 6.Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (2005) Virus Taxonomy, 8th edn. Elsevier Academic Press, New YorkGoogle Scholar
- 17.Maynard Smith J (1992) Analyzing the mosaic structure of genes. J Mol Evol 34:126–129Google Scholar
- 18.Owor BE, Shepherd DN, Taylor NJ, Edema R, Monjane AL, Thomson JA, Martin DP, Varsani A (2007) Successful application of FTA classic card technology and use of bacteriophage phi29 DNA polymerase for large-scale field sampling and cloning of complete maize streak virus genomes. J Virol Methods 140:100–105PubMedCrossRefGoogle Scholar