Archives of Virology

, Volume 152, Issue 4, pp 819–825 | Cite as

Molecular analysis of a full-length sequence of a new yam badnavirus from Dioscorea sansibarensis

  • S. Seal
  • E. Muller
Brief Report


Badnavirus-like particles were observed by ISEM in viral preparations from yam (Dioscorea sansibarensis) leaves from Benin. Use of the viral preparation as template for PCR amplification with badnavirus-specific primers gave rise to a 579-bp product with most nucleotide identity (70.8%) to Dioscorea alata bacilliform virus (DaBV, Accession numbers X94575–X94582), the only other yam badnavirus sequenced to date. A full-length badnavirus sequence was generated, which consisted of 7261 nucleotides with a typical Badnavirus genome organisation. The full-length sequence shared most identity (61.9%) to DaBV (Accession numbers X94575–X94582) and hence represents a member of a new badnavirus species termed Dioscorea sansibarensis bacilliform virus (DsBV).


Rice Tungro Bacilliform Virus Natural Resource Institute Banana Streak Virus Total Plant DNAs Cacao Swell Shoot Virus 
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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • S. Seal
    • 1
  • E. Muller
    • 2
  1. 1.Natural Resources InstituteThe University of Greenwich at MedwayChatham MaritimeU.K.
  2. 2.CIRAD, UMR BGPIMontpellierFrance

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