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Archives of Virology

, Volume 152, Issue 4, pp 819–825 | Cite as

Molecular analysis of a full-length sequence of a new yam badnavirus from Dioscorea sansibarensis

  • S. Seal
  • E. Muller
Brief Report

Summary

Badnavirus-like particles were observed by ISEM in viral preparations from yam (Dioscorea sansibarensis) leaves from Benin. Use of the viral preparation as template for PCR amplification with badnavirus-specific primers gave rise to a 579-bp product with most nucleotide identity (70.8%) to Dioscorea alata bacilliform virus (DaBV, Accession numbers X94575–X94582), the only other yam badnavirus sequenced to date. A full-length badnavirus sequence was generated, which consisted of 7261 nucleotides with a typical Badnavirus genome organisation. The full-length sequence shared most identity (61.9%) to DaBV (Accession numbers X94575–X94582) and hence represents a member of a new badnavirus species termed Dioscorea sansibarensis bacilliform virus (DsBV).

Keywords

Rice Tungro Bacilliform Virus Natural Resource Institute Banana Streak Virus Total Plant DNAs Cacao Swell Shoot Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • S. Seal
    • 1
  • E. Muller
    • 2
  1. 1.Natural Resources InstituteThe University of Greenwich at MedwayChatham MaritimeU.K.
  2. 2.CIRAD, UMR BGPIMontpellierFrance

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