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Archives of Virology

, Volume 152, Issue 3, pp 575–584 | Cite as

Analysis of hepatitis B surface antigen mutations in Mongolia: Molecular epidemiology and implications for mass vaccination

  • D. Davaalkham
  • T. Ojima
  • R. Uehara
  • M. Watanabe
  • I. Oki
  • K. Endo
  • M. Takahashi
  • H. Okamoto
  • Y. Nakamura
Article

Summary.

Although the potential significance of hepatitis B surface antigen (HBsAg) mutants for failure of immunization has been studied in some endemic countries, whether the “a” determinant variants are responsible for vaccine failure in Mongolia remains unknown. Fifty-nine HBsAg-positive children (age: 8.8 ± 0.9 years) who had been observed during the nationwide survey of vaccinated cohorts conducted in 2004 were subjected to molecular analyses of hepatitis B virus (HBV). Partial S gene sequences encoding amino acids (aa) 40–171 of HBsAg were determined in 57 children (96.6%) who had detectable HBV DNA. Phylogenetic analysis of the S gene sequences revealed that genotype D accounted for 93.0% and genotype A for 5.3%. Only one child (1.7%) had HBVs of genotypes A and D. HBsAg mutations were found in 17 (29.8%) children ranging from 1 to 4 aa per subject (mean ± SD, 1.6 ± 0.9 aa). Pro127Thr and Thr118Ala were the most common substitutions, which occurred in 6 (10.5%) and 3 (5.3%) subjects, respectively; none had Gly145Arg. There were no significant associations in the prevalence of HBsAg mutations with age, sex, residential area, or vaccination status against hepatitis B. Analysis of the deduced amino acid sequence of the entire preS1/preS2/S gene revealed that eight genotype D isolates and one genotype A isolate were quite similar to previously-reported wild-type isolates, suggesting that they are essentially wild-type, but not vaccine-induced mutants. In conclusion, the results demonstrate that hepatitis B surface gene mutants do not play a significant role in vaccination failure in Mongolia.

Keywords

Hepatitis Delta Virus Surface Antigen Variant HBsAg Mutant Entire preS1 Surface Gene Mutant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • D. Davaalkham
    • 1
    • 2
  • T. Ojima
    • 3
  • R. Uehara
    • 1
  • M. Watanabe
    • 1
  • I. Oki
    • 1
  • K. Endo
    • 4
  • M. Takahashi
    • 4
  • H. Okamoto
    • 4
  • Y. Nakamura
    • 1
  1. 1.Department of Public HealthJichi Medical University School of MedicineTochigiJapan
  2. 2.Department of Epidemiology and BiostatisticsHealth Sciences University of MongoliaUlaanbaatarMongolia
  3. 3.Department of Community Health and Preventive MedicineHamamatsu University School of MedicineShizuokaJapan
  4. 4.Division of Virology, Department of Infection and ImmunityJichi Medical University School of MedicineTochigiJapan

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