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Archives of Virology

, Volume 152, Issue 1, pp 25–39 | Cite as

Mapping of B cell epitopes in measles virus nucleocapsid protein

  • A. Zvirbliene
  • I. Kucinskaite
  • I. Sezaite
  • D. Samuel
  • K. Sasnauskas
Article

Summary.

The B-cell response against measles nucleoprotein (MeN) plays an important role in the control of measles infection. However, the data on B cell epitopes of MeN are still limited. The objective of this study was to identify B cell epitopes in MeN using monoclonal and polyclonal antibodies raised against recombinant yeast-expressed MeN (rMeN) as well as human sera from measles-positive individuals. After immunization of mice, 15 monoclonal antibodies (mAbs) against rMeN were generated. The B cell epitopes were localized using recombinant overlapping MeN fragments, PepScan analysis, and competitive ELISA. The epitopes of 14 mAbs were mapped within the C-terminus of MeN between amino acids (aa) 419 and 525. Four mAbs recognized a linear epitope located within a sequence of aa 440–448. Competitive ELISA revealed a cluster of conformational mAb epitopes. Cross-inhibition studies with human sera demonstrated similar localization of B cell epitopes recognized by serum antibodies from naturally infected individuals. Thus, the majority of B cell epitopes are located at the C-terminal domain of MeN. These findings provide new data on the antigenic structure of MeN and are in agreement with recent experimental evidence indicating that the C-terminal domain of MeN is well accessible on the surface of nucleocapsid-like structures.

Keywords

Measle Cell Epitope Measle Virus Human Seron Competitive ELISA 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • A. Zvirbliene
    • 1
  • I. Kucinskaite
    • 1
  • I. Sezaite
    • 1
  • D. Samuel
    • 2
  • K. Sasnauskas
    • 1
  1. 1.Institute of BiotechnologyVilniusLithuania
  2. 2.Enteric, Respiratory and Neurological Virus LaboratoryHealth Protection AgencyLondonUK

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