Archives of Virology

, Volume 152, Issue 1, pp 85–96 | Cite as

Molecular and biological comparison of two Tomato yellow ring virus (TYRV) isolates: challenging the Tospovirus species concept

  • A. Hassani-Mehraban
  • J. Saaijer
  • D. Peters
  • R. Goldbach
  • R. Kormelink


Two strains of Tomato yellow ring virus (TYRV, genus Tospovirus), one from tomato (referred to as TYRV-t) and the other from soybean and potato (denoted TYRV-s), collected from different geographical regions in Iran, were compared. Their genomic S RNA segments differed in size by 55 nucleotides. Comparison of the S RNA intergenic regions revealed the absence of a stretch of 115 nucleotides within the S RNA segment of TYRV-s and, conversely, of 56 nts in that of TYRV-t, apparently a stable genetic difference as it was also found in another isolate of TYRV-s collected from potato. Sequence comparison of the N protein ORFs revealed an identity of 92% between the N proteins of both strains, and the observed strong cross-reaction of TYRV-s in DAS-ELISA with a polyclonal antiserum directed against the TYRV-t N protein confirmed this high identity. Host range analysis revealed several differences, e.g. TYRV-s, but not TYRV-t, being able to systemically infect Nicotiana species, and TYRV-s being localised in tomato. The observed molecular and biological differences of both viruses call into question the currently used criteria for Tospovirus species demarcation.


Reverse Transcriptase Polymerase Chain Reaction Tomato Spotted Wilt Virus Tomato Spot Wilt Virus Iris Yellow Spot Virus Impatiens Necrotic Spot Virus 
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  1. Bananej, K, Shahraeen, N, Ahoonmanesh, A, Lesemann, DE, Shahriary, D 1998Identification of Tomato spotted wilt virus from tomato fields in Varamin area. (English abstr.)Iran J Plant Pathol343036Google Scholar
  2. Bucher, E, Sijen, T, De Haan, P, Goldbach, R, Prins, M 2003Negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positionsJ Virol7713291336PubMedCrossRefGoogle Scholar
  3. Cortêz, I, Livieratos, IC, Derks, A, Peters, D, Kormelink, R 1998Molecular and serological characterization of Iris yellow spot virus, a new and distinct tospovirus speciesPhytopathology8812761282Google Scholar
  4. Cortês, I, Saaijer, J, Wongjkaew, KS, Pereira, A-M, Goldbach, R, Peters, D, Kormelink, R 2001Identification and characterization of a novel tospovirus species using a new RT-PCR approachArch Virol146265278CrossRefGoogle Scholar
  5. De Ávila, AC, de Haan, P, Kormelink, R, Resende, R de O, Goldbach, R, Peters, D 1993Classification of tospoviruses based on phylogeny of nucleoprotein gene sequencesJ Gen Virol74153159PubMedGoogle Scholar
  6. De Ávila, AC, de Haan, P, Smeets, MLL, Resende, R de O, Kormelink, R, Kitajima, EW, Goldbach, RW, Peters, D 1993Distinct levels of relationships between tospovirus isolatesArch Virol128211227PubMedCrossRefGoogle Scholar
  7. De Ávila, AC, Huguenot, C, Resende, R, Kitajiama, EW, Goldbach, RW, Peters, D 1990Serological differentiation of twenty isolates of tomato spotted wilt virusJ Gen Virol7128012807PubMedGoogle Scholar
  8. De Haan, P, Kormelink, R, Resende, R de O, van Poelwijk, F, Peters, D, Goldbach, R 1991Tomato spotted wilt virus L RNA encodes a putative RNA-polymeraseJ Gen Virol7222072216PubMedGoogle Scholar
  9. De Haan, P, Wagemakers, L, Peters, D, Goldbach, R 1990The S RNA segment of tomato spotted wilt virus has an ambisense characterJ Gen Virol7110011007PubMedGoogle Scholar
  10. Fauquet, CM, Mayo, MA, Maniloff, J, Desselberger, U, Ball, LA 2005Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of VirusesAcademic PressNew York1162Google Scholar
  11. Goldbach, R, Kuo, G 1996Introduction: Proceedings of the international symposium on tospovirus and thrips of floral and vegetable cropsActa Hortic4312126Google Scholar
  12. Ghotbi, T, Shahraeen, N, Winter, S 2005Occurrence of tospoviruses in ornamental and weed species in Markazi and Tehran provinces in IranPlant Dis89425429Google Scholar
  13. Golnaraghi, AR, Pourrahim, R, Shahraeen, N, Farzadfar, Sh 2002First report of Groundnut bud necrosis virus in Iran. (Dis. Notes)Plant Dis86561Google Scholar
  14. Golnaraghi, AR, Shahraeen, N, Pourrahim, R, Farzadfar, Sh, Ghasemi, A 2004Occurrence and relative incidence of viruses infecting soybeans in IranPlant Dis8810691074Google Scholar
  15. Hassani-Mehraban, A, Saaijer, J, Peters, D, Goldbach, R, Kormelink, R 2005A new tomato-infecting tospovirus from IranPhytopathology95852858Google Scholar
  16. Heinze, C, Letschert, B, Hristova, D, Yankulova, M, Kauadjouor, O, Willingmann, P, Atanassov, A, Adam, G 2001Variability of the N-protein and the intergenic region of the S RNA of tomato spotted wilt tospovirus (TSWV)N Microbiol24175187Google Scholar
  17. Heinze, C, Willingmann, , Schwach, F, Adam, G 2003An unusual large intergenic region in the S-RNA of a Bulgarian tomato spotted wilt virus isolateArch Virol148199205PubMedCrossRefGoogle Scholar
  18. Inoue-Nagata, AK, Kormelink, R, Nagata, T, Kitajima, EW, Goldbach, R, Peters, D 1997Effects of temperature and host on the generation of Tomato spotted wilt virus defective interfering RNAsPhytopathology8711681173Google Scholar
  19. Kormelink, R, de Haan, P, Meurs, C, Peters, D, Goldbach, R 1992The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segmentsJ Gen Virol7327952804PubMedCrossRefGoogle Scholar
  20. Kormelink, R, Storms, M, Van Lent, J, Peters, D, Goldbach, R 1994Expression and subcellular location of the NSm protein of tomato spotted wilt virus (TSWV), a putative viral movement proteinVirology2005665PubMedCrossRefGoogle Scholar
  21. McMichael, AL, Perley, DM, Thomas, JE 2002A new tospovirus serogroup IV species infecting capsicum and tomato in Queensland, AustraliaAustralasian Plant Pathol31231239CrossRefGoogle Scholar
  22. Mumford, RA, Seal, SE 1997Rapid single immunocapture RTPCR for the detection of two yam potyvirusesJ Virol Methods697379PubMedCrossRefGoogle Scholar
  23. Okuda, M, Taba, S, Hanada, K 2003The S RNA segment determines symptom differences on Tetragonia expansa between two Watermelon silver mottle virus isolatesPhysiol Mol Plant Pathol62327332CrossRefGoogle Scholar
  24. Ohnishi, J, Katsuzaki, H, Tsuda, S, Sakurai, T, Akutsu, K, Murai, T 2006 Frankliniella cephalica, a new vector for Tomato spotted wilt virusPlant Dis90685Google Scholar
  25. Pappu, SS, Bhat, AI, Pappu, HR, Deom, CM, Culbreath, AK 2000Phylogenetic studies of tospoviruses (Family: Bunyaviridae) based on intergenic region sequence of small and medium genomic RNAsArch Virol14510351045PubMedCrossRefGoogle Scholar
  26. Pozzer, L, Bezzera, I, Kormelink, R, Prins, M, Peters, D, Resende, R de O, de Ávila, AC 1999Characterization of a distinct tospovirus isolate of Iris yellow spot virus associated with a disease in onion fields in BrazilPlant Dis83345350Google Scholar
  27. Premachandra, WTSD, Borgemeister, C, Maiss, E, Knierim, D, Poehling, H-M 2005Ceratothripoides claratris, a new vector of a Capsicum chlorosis virus isolate infecting tomato in ThailandPhytopathology95659663Google Scholar
  28. Qui, WP, Geske, SM, Hickey, CM, Moyer, JW 1998Tomato spotted wilt tospovirus genome reassortment and genome segment-specific adaptationVirology244186194CrossRefGoogle Scholar
  29. Sanger, F, Nicklen, S, Coulson, AR 1977DNA sequencing with chain-terminating inhibitorsProc Natl Acad Sci USA7454635467PubMedCrossRefGoogle Scholar
  30. Shahraeen, N, Ghotbi, T, Mehraban, AH 2002Occurrence of Impatiens necrotic spot virus in ornamentals in Mahallat and Tehran provinces in Iran. (Dis. Notes)Plant Dis86694Google Scholar
  31. Swofford DL (1993) PAUP: Phylogenetic analysis using parsimony. Version 3.1.1. Computer program distributed by the Illinois Natural History Survey, Champaign, ILGoogle Scholar
  32. Takeda, A, Sugiyama, K, Nagano, H, Mori, M, Kaido, M, Mise, K, Tsuda, S, Okuno, T 2002Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virusFEBS Lett5327579PubMedCrossRefGoogle Scholar
  33. Thompson, JD, Higgins, DG, Gibson, TJ 1994CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Res2246734680PubMedGoogle Scholar
  34. Van Knippenberg, I, Goldbach, R, Kormelink, R 2005 Tomato spotted wilt virus S-segment mRNAs have overlapping 3′ ends containing a predicted stem-loop structure and conserved sequence motifVir Res110125131CrossRefGoogle Scholar
  35. Wang, M, Gonsalves, D 1990ELISA detection of various tomato spotted wilt virus isolates using specific antisera to structural proteins of the virusPlant Dis74154158Google Scholar
  36. Whitfield, AE, Ullman, DE, German, TL 2005Tospovirus-thrips interactionsAnnu Rev Phytopathol43459489PubMedCrossRefGoogle Scholar
  37. Zuker, M 2003Mfold web server for nucleic acid folding and hybridization predictionNucleic Acids Res3134063415PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • A. Hassani-Mehraban
    • 1
  • J. Saaijer
    • 1
  • D. Peters
    • 1
  • R. Goldbach
    • 1
  • R. Kormelink
    • 1
  1. 1.Laboratory of VirologyWageningen UniversityWageningenThe Netherlands

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