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Archives of Virology

, Volume 152, Issue 1, pp 85–96 | Cite as

Molecular and biological comparison of two Tomato yellow ring virus (TYRV) isolates: challenging the Tospovirus species concept

  • A. Hassani-Mehraban
  • J. Saaijer
  • D. Peters
  • R. Goldbach
  • R. Kormelink
Article

Summary.

Two strains of Tomato yellow ring virus (TYRV, genus Tospovirus), one from tomato (referred to as TYRV-t) and the other from soybean and potato (denoted TYRV-s), collected from different geographical regions in Iran, were compared. Their genomic S RNA segments differed in size by 55 nucleotides. Comparison of the S RNA intergenic regions revealed the absence of a stretch of 115 nucleotides within the S RNA segment of TYRV-s and, conversely, of 56 nts in that of TYRV-t, apparently a stable genetic difference as it was also found in another isolate of TYRV-s collected from potato. Sequence comparison of the N protein ORFs revealed an identity of 92% between the N proteins of both strains, and the observed strong cross-reaction of TYRV-s in DAS-ELISA with a polyclonal antiserum directed against the TYRV-t N protein confirmed this high identity. Host range analysis revealed several differences, e.g. TYRV-s, but not TYRV-t, being able to systemically infect Nicotiana species, and TYRV-s being localised in tomato. The observed molecular and biological differences of both viruses call into question the currently used criteria for Tospovirus species demarcation.

Keywords

Reverse Transcriptase Polymerase Chain Reaction Tomato Spotted Wilt Virus Tomato Spot Wilt Virus Iris Yellow Spot Virus Impatiens Necrotic Spot Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • A. Hassani-Mehraban
    • 1
  • J. Saaijer
    • 1
  • D. Peters
    • 1
  • R. Goldbach
    • 1
  • R. Kormelink
    • 1
  1. 1.Laboratory of VirologyWageningen UniversityWageningenThe Netherlands

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