Advertisement

Archives of Virology

, Volume 151, Issue 11, pp 2289–2299 | Cite as

Genetic characterization of novel reassortant H1N2 influenza A viruses isolated from pigs in southeastern China

  • X. Qi
  • C. P. Lu
Brief Report

Summary.

In December 2004, three influenza H1N2 viruses were isolated from lung samples of pigs that had died from respiratory disease on a farm in southeastern China. To determine the genetic characterization and probable origin, one of the three isolates, A/Swine/Zhejiang/1/2004 (Sw/ZJ/1/2004), was genetically analyzed. Sw/ZJ/1/2004 was a reassortant with an NA gene most closely related to the corresponding gene from a human-like H3N2 virus circulating in 1995. The remaining seven genes were most closely related to those from the classical swine H1N1 virus. Sw/ZJ/1/2004 appeared to be a novel reassortant H1N2 virus that was genetically distinguishable from other H1N2 viruses found in pigs worldwide. The isolation of Sw/ZJ/1/2004 provided further evidence for pigs serving as a “mixing vessel” for the generation of new reassortant genotypes of influenza viruses and emphasizes the importance of reinforcing influenza virus surveillance in pigs in China.

Keywords

H1N2 Virus Avian Virus Reassortant Virus Human H3N2 Virus Reassortant H1N2 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brown, IH 2000The epidemiology and evolution of influenza viruses in pigsVet Microbiol742946PubMedCrossRefGoogle Scholar
  2. Brown, IH, Chakraverty, P, Harris, PA, Alexander, DJ 1995Disease outbreaks in pigs in Great Britain due to an influenza A virus of H1N2 subtypeVet Rec136328329PubMedGoogle Scholar
  3. Brown, IH, Harris, PA, McCauley, JW, Alexander, DJ 1998Multiple genetic reassortment of avian and human influenza A viruses in European pigs, resulting in emergence of an H1N2 virus of novel genotypeJ Gen Virol7929472955PubMedGoogle Scholar
  4. Caton, AJ, Brownlee, GG, Yewdell, JW, Gerhard, W 1982The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (H1 subtype)Cell31417427PubMedCrossRefGoogle Scholar
  5. Choi, YK, Goyal, SM, Kang, SW, Farnham, MW, Joo, HS 2002Detection and subtyping of swine influenza H1N1, H1N2 and H3N2 viruses in clinical samples using two multiplex RT-PCR assaysJ Virol Methods1025359PubMedCrossRefGoogle Scholar
  6. Fouchier, RAM, Munster, V, Wallensten, A, Bestebroer, TM, Herfst, S, Smith, D, Rimmelzwaan, GF, Olsen, B, Osterhaus, ADME 2005Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gullsJ Virol7928142822PubMedCrossRefGoogle Scholar
  7. Gourreau, JM, Kaiser, C, Valette, M, Douglas, AR, Labie, J, Aymard, M 1994Isolation of two H1N2 influenza viruses from swine in FranceArch Virol135365382PubMedCrossRefGoogle Scholar
  8. Guan, Y, Shortridge, KF, Krauss, S, Li, PH, Kawaoka, Y, Webste, RG 1996Emergence of avian H1N1 influenza viruses in pigs in ChinaJ Virol7080418046PubMedGoogle Scholar
  9. Guo, Y, Xu, X, Cox, NJ 1992Human influenza A (H1N2) viruses isolated from ChinaJ Gen Virol73383388PubMedGoogle Scholar
  10. Hoffmann, E, Stech, J, Guan, Y, Webster, RG, Perez, DR 2001Universal primer set for the full-length amplification of all influenza A virusesArch Virol14622752289PubMedCrossRefGoogle Scholar
  11. Inkster, MD, Hinshaw, VS, Schulze, IT 1993The hemagglutinins of duck and human H1 influenza viruses differ in sequence conservation and in glycosylationJ Virol6774367443PubMedGoogle Scholar
  12. Ito, T, Kawaoka, Y, Vines, A, Ishikawa, H, Asai, T, Kida, H 1998Continued circulation of reassortment H1N2 influenza viruses in pigs in JapanArch Virol14317731782PubMedCrossRefGoogle Scholar
  13. Jung, K, Chae, C 2004Phylogenetic analysis of an H1N2 influenza A virus isolated from a pig in KoreaArch Virol14914151422PubMedCrossRefGoogle Scholar
  14. Karasin, AI, Anderson, GA, Olsen, CW 2000Genetic characterization of an H1N2 influenza virus isolated from a pig in IndianaJ Clin Microbiol3824532456PubMedGoogle Scholar
  15. Karasin, AI, West, K, Carman, S, Olsen, CW 2004Characterization of avian H3N3 and H1N1 influenza A viruses isolated from pigs in CanadaJ Clin Microbiol4243494354PubMedCrossRefGoogle Scholar
  16. Lipatov, AS, Govorkova, EA, Webby, RJ, Ozaki, H, Peiris, M, Guan, Y, Poon, L, Webster, RG 2004Influenza: emergence and controlJ Virol7889518959PubMedCrossRefGoogle Scholar
  17. Matrosovich, M, Tuzikov, A, Bovin, N, Gambaryan, A, Klimov, A, Castrucci, M, Donatelle, I, Kawaoka, Y 2000Early alterations of the receptor-binding properties of H1, H2, and H3 avian influenza virus hemagglutinins after their introduction into mammalsJ Virol7485028512PubMedCrossRefGoogle Scholar
  18. Nerome, K, Ishida, M, Oya, A, Oda, K 1982The possible origin H1N1 (Hsw1N1) virus in the swine population of Japan and antigenic analysis of the isolatesJ Gen Virol62171175PubMedGoogle Scholar
  19. Nerome, K, Kanegae, Y, Shortridge, KF, Sugita, S, Ishida, M 1995Genetic analysis of porcine H3N2 viruses originating in southern ChinaJ Gen Virol76613624PubMedGoogle Scholar
  20. Ninomiya, A, Takada, A, Okazaki, K, Shortridge, KF, Kida, H 2002Seroepidemiological evidence of avian H4, H5, and H9 influenza A virus transmission to pigs in southeastern ChinaVet Microbiol88107114PubMedCrossRefGoogle Scholar
  21. Noble, S, McGregor, MS, Webster, DE, Hinshaw, VS 1993Antigenic and genetic conservation of the haemagglutinin in H1N1 swine influenza virusesJ Gen Virol7411971200PubMedGoogle Scholar
  22. Olsen, CW, McGregor, MW, Cooley, AJ, Schantz, B, Hotze, B, Hinshaw, VS 1993Antigenic and genetic analysis of a recently isolated H1N1 swine influenza virusAm J Vet Res5416301636PubMedGoogle Scholar
  23. Olsen, CW, Karasin, A, Erickson, G 2003Characterization of a swine-like reassortant H1N2 influenza virus isolated from a wild duck in the United StatesVirus Res93115121PubMedCrossRefGoogle Scholar
  24. Ouchi, A, Nerome, K, Kanegae, Y, Ishida, M, Nerome, R, Hayashi, K, Hashimoto, T, Kaju, M, Kagi, Y, Inaba, Y 1996Large outbreak of swine influenza in southern Japan caused by reassortant (H1N2) influenza viruses: its epizootic background and characterization of the causative virusesJ Gen Virol7717511759PubMedCrossRefGoogle Scholar
  25. Peiris, JSM, Guan, Y, Markwell, D, Ghose, P, Webster, RG, Shortridge, KF 2001Cocirculation of avian H9N2 and contemporary “human” H3N2 influenza A viruses in pigs in southeastern China: potential for genetic reassortment?J Virol7596799686PubMedCrossRefGoogle Scholar
  26. Schulze IT (1997) Effects of glycosylation on the properties and functions of influenza virus hemagglutinin. J Infect Dis Suppl: S24–S28Google Scholar
  27. Shu, LL, Lin, YP, Wright, SM, Shortridge, KF, Webster, RG 1994Evidence for interspecies transmission and reassortment of influenza A viruses in pigs in southern ChinaVirology202825833PubMedCrossRefGoogle Scholar
  28. Webby, RJ, Swenson, SL, Krauss, SL, Gerrish, PJ, Goyal, SM, Webster, RG 2000Evolution of swine H3N2 influenza viruses in the United StatesJ Viro7482438251CrossRefGoogle Scholar
  29. Webster, RG, Bean, WJ, Gorman, OT, Chambers, TM, Kawaoka, Y 1992Evolution and Ecology of Influenza A VirusesMicrobiological Reviews56152179PubMedGoogle Scholar
  30. Weis, W, Brown, JH, Cusack, S, Paulson, JC, Skehel, JJ, Wiley, DC 1988Structure of the influenza virus haemagglutinin complexed with its receptor sialic acidNature333426431PubMedCrossRefGoogle Scholar
  31. Winter, G, Fields, S, Brownlee, GG 1981Nucleotide sequence of the haemagglutinin gene of a human influenza virus H1 subtypeNature2927275PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • X. Qi
    • 1
  • C. P. Lu
    • 1
  1. 1.College of Veterinary Medicine, Nanjing Agricultural UniversityNanjingP.R. China

Personalised recommendations