Archives of Virology

, Volume 151, Issue 2, pp 273–283 | Cite as

Mapping the RNA-binding domain on the DpCPV VP4

  • S. L. Zhao
  • C. Y. Liang
  • W. J. Zhang
  • X. C. Tang
  • H. Y. Peng
Article

Summary.

The RNA-binding properties of VP4 protein of Dendrolimus punctatus cytoplasmic polyhedrosis virus (DpCPV) VP4 were analyzed. VP4 was expressed in E. coli and assayed for RNA binding activity by gel mobility shift assay. VP4 was found to bind RNA (ssRNA and dsRNA) in a sequence-independent manner, but did not interact with DNA. To identify the domain(s) of the protein important for RNA binding, a number of deletions were made and tested by gel mobility shift assays and northwestern blot. The central region of VP4 from amino acid residues 77 to 155 was found to contain the RNA binding domain.

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Copyright information

© Springer-Verlag/Wien 2005

Authors and Affiliations

  • S. L. Zhao
    • 1
    • 2
    • 3
  • C. Y. Liang
    • 2
    • 3
  • W. J. Zhang
    • 1
    • 2
    • 3
  • X. C. Tang
    • 1
    • 2
  • H. Y. Peng
    • 1
    • 2
  1. 1.Laboratory for Biological Control, Wuhan Institute of Virology, Chinese Academy of SciencesWuhanP.R. China
  2. 2.State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesWuhanP.R. China
  3. 3.Graduate School of the Chinese Academy of SciencesBeijingP.R. China

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