Deletion analysis of the sapovirus VP1 gene for the assembly of virus-like particles
Summary.
Human sapovirus (SaV) strains are agents of gastroenteritis. They cannot be grown in cell culture. In this study, constructs containing SaV N- and C-terminal-deleted recombinant capsid proteins (rVP1) were expressed in a baculovirus expression system to allow us to better understand the sequence requirements for the formation of virus-like particles (VLPs). Only proteins derived from N-terminal-deleted rVP1 constructs that began 49 nucleotides downstream assembled into VLPs, which included both small and native-size VLPs. Our results were similar to those reported in a rabbit hemorrhagic disease virus (RHDV) N- and C-terminal-deleted rVP1 expression study but were distinct from those reported in a norovirus N- and C-terminal-deleted rVP1 expression study, suggesting that SaV and RHDV may have similar expression requirements.
Keywords
Nucleotide Cell Culture Infectious Disease Expression System Disease VirusPreview
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References
- Barcena, J, Verdaguer, N, Roca, R, Morales, M, Angulo, I, Risco, C, Carrascosa, JL, Torres, JM, Caston, JR 2004The coat protein of rabbit hemorrhagic disease virus contains a molecular switch at the N-terminal region facing the inner surface of the capsid.Virology322118134CrossRefPubMedGoogle Scholar
- Bertolotti-Ciarlet, A, White, LJ, Chen, R, Prasad, BV, Estes, MK 2002Structural requirements for the assembly of Norwalk virus-like particles.J Virol7640444055CrossRefPubMedGoogle Scholar
- Bertolotti-Ciarlet, A, Crawford, SE, Hutson, AM, Estes, MK 2003The 3′ end of Norwalk virus mRNA contains determinants that regulate the expression and stability of the viral capsid protein VP1: a novel function for the VP2 protein.J Virol771160311615CrossRefPubMedGoogle Scholar
- Caul, EO, Appleton, H 1982The electron microscopical and physical characteristics of small round human fecal viruses: an interim scheme for classification.J Med Virol9257265PubMedGoogle Scholar
- Chen, R, Neill, JD, Noel, JS, Hutson, AM, Glass, RI, Estes, MK, Prasad, BV 2004Inter- and intragenus structural variations in caliciviruses and their functional implications.J Virol7864696479PubMedGoogle Scholar
- Green, KY, Ando, T, Balayan, MS, Berke, T, Clarke, IN, Estes, MK, Matson, DO, Nakata, S, Neill, JD, Studdert, MJ, Thiel, HJ 2000Taxonomy of the caliciviruses.J Infect Dis181S322S330PubMedGoogle Scholar
- Hansman, G, Doan, LP, Kguyen, T, Okitsu, S, Katayama, K, Ogawa, S, Natori, K, Takeda, N, Kato, Y, Nishio, O, Noda, M, Ushijima, H 2004Detection of norovirus and sapovirus infection among children with gastroenteritis in Ho Chi Minh City, Vietnam.Arch Virol14916731688CrossRefPubMedGoogle Scholar
- Hansman, GS, Katayama, K, Maneekarn, N, Peerakome, S, Khamrin, P, Tonusin, S, Okitsu, S, Nishio, O, Takeda, N, Ushijima, H 2004Genetic diversity of norovirus and sapovirus in hospitalized infants with sporadic cases of acute gastroenteritis in Chiang Mai, Thailand.J Clin Microbiol4213051307CrossRefPubMedGoogle Scholar
- Hansman, GS, Katayama, K, Oka, T, Natori, K, Takeda, N 2005Mutational study of sapovirus expression in insect cells.Virol J213CrossRefPubMedGoogle Scholar
- Hansman, GS, Natori, K, Oka, T, Ogawa, S, Tanaka, K, Nagata, N, Ushijima, H, Takeda, N, Katayama, K 2005Cross-reactivity among sapovirus recombinant capsid proteins.Arch Virol1502136PubMedGoogle Scholar
- Prasad, BV, Hardy, ME, Dokland, T, Bella, J, Rossmann, MG, Estes, MK 1999X-ray crystallographic structure of the Norwalk virus capsid.Science286287290CrossRefPubMedGoogle Scholar
- White, LJ, Hardy, ME, Estes, MK 1997Biochemical characterization of a smaller form of recombinant Norwalk virus capsids assembled in insect cells.J Virol7180668072PubMedGoogle Scholar