Archives of Virology

, Volume 150, Issue 10, pp 2109–2116 | Cite as

The complete sequence of a Spanish isolate of Broad bean wilt virus 1 (BBWV-1) reveals a high variability and conserved motifs in the genus Fabavirus

  • R. M. Ferrer
  • J. Guerri
  • M. S. Luis-Arteaga
  • P. Moreno
  • L. Rubio
Brief Report


The genome of a Spanish isolate of Broad bean wilt virus-1 (BBWV-1) was completely sequenced and compared with available sequences of other isolates of the genus Fabavirus (BBWV-1 and BBWV-2). This consisted of two RNAs of 5814 and 3431 nucleotides, respectively, and their organization was similar to that of other members of the family Comoviridae. Its mean nucleotide identity with a BBWV-1 American isolate was 81.5%, and between 59.8 and 63.5% with seven BBWV-2 isolates. Our analysis showed sequence stretches in the 5′ non-coding regions which are conserved in both genomic RNAs and in BBWV-1 and BBWV-2 isolates.


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  1. Froussard, P 1992A random-PCR method (rPCR) to construct whole cDNA library from low amounts of RNA.Nucleic Acids Res202900PubMedGoogle Scholar
  2. Goldbach RW, Wellink J (1996) Comoviruses: molecular biology and replication. In: Harrison BH, Murant AF (eds), Polyhedral virions and bipartite RNA genomes: the plant viruses, vol. 5. Plenum Press, New York, pp 35–76Google Scholar
  3. Ikegami, M, Kawashima, H, Natsuaki, T, Sugimura, N 1998Complete nucleotide sequence of the genome organization of RNA2 of patchouli mild mosaic virus, a new fabavirus.Arch Virol14324312434CrossRefPubMedGoogle Scholar
  4. Ikegami, M, Onobori, Y, Sugimura, N, Natsuaki, T 2001Complete nucleotide sequence and the genome organization of Patchouli mild mosaic virus RNA1.Intervirology44355358CrossRefPubMedGoogle Scholar
  5. Joshi, CP, Zhou, H, Huang, X, Chiang, VL 1997Context sequence of translation initiation codon in plants.Plant Mol Biol359931001CrossRefPubMedGoogle Scholar
  6. Kadaré, G, Haenni, A 1997Virus-encoded helicases.J Virol7125832590PubMedGoogle Scholar
  7. Kobayashi, YO, Nakano, M, Kashiwazaki, S, Naito, T, Mikoshiba, Y, Shiota, A, Kameya-Iwaki, M, Honda, Y 1999Sequence analysis of RNA-2 of different isolates of broad bean wilt virus confirms the existence of two distinct species.Virol14414291438CrossRefGoogle Scholar
  8. Kobayashi, YO, Kobayashi, A, Nakano, M, Hagiwara, K, Honda, Y, Omura, T 2003Analysis of genetic relations between Broad bean wilt virus 1 and Broad bean wilt virus 2.J Gen Plant Pathol69320326CrossRefGoogle Scholar
  9. Koh, LH, Cooper, JI, Wong, SM 2001Complete sequences and phylogenetic analyses of a Singapore isolate of broad bean wilt fabavirus.Arch Virol146135147CrossRefPubMedGoogle Scholar
  10. Koonin, EV, Dolja, VV 1993Evolution and taxonomy of positive strand RNA viruses: implications of comparative analysis of amino acid sequences.Crit Rev Biochem Mol Biol28375430PubMedGoogle Scholar
  11. Kumar, S, Tamura, K, Jakobsen, IB, Nei, M 2001MEGA 2: Molecular Evolutionary Genetics Analysis software.Bioinformatics1712441245CrossRefPubMedGoogle Scholar
  12. Kuroda, T, Okumura, A, Takeda, I, Miura, Y, Suzuki, K 2000Nucleotide sequence and synthesis of infectious RNA from cloned cDNA of broad bean wilt virus 2 RNA 2.Arch Virol145787793CrossRefPubMedGoogle Scholar
  13. Lai, MMC 1998Cellular factors in the transcription and replication of viral RNA genomes: a parallel to DNA-dependent RNA transcription.Virology244112CrossRefPubMedGoogle Scholar
  14. Lee, U, Hong, JS, Choi, JK, Kim, KC, Kim, YS, Curtis, IS, Nam, HG, Lim, PO 2000Broad bean wilt virus causes necrotic symptoms and generates defective RNAs in Capsicum annuum.Phytopathology9013901395Google Scholar
  15. Lisa V, Boccardo G (1996) Fabaviruses: Broad bean wilt virus and allied viruses. In: Harrison BH, Murant AF (eds), Polyhedral virions and bipartite RNA genomes: the plant viruses, vol. 5. Plenum Press, New York, pp 229–250Google Scholar
  16. Melcher, U 2000The ‘30K superfamily’ of viral movement proteins.J Gen Virol81257266PubMedGoogle Scholar
  17. Mayo, MA, Fritsch, C 1994A possible consensus sequence for VPg of viruses in the family Comoviridae.FEBS Lett354129130CrossRefPubMedGoogle Scholar
  18. Moreno, P, Guerri, J, Muñoz, N 1990Identification of Spanish strains of citrus tristeza virus (CTV) by analysis of double-stranded RNAs (dsRNA).Phytopahology80477482Google Scholar
  19. Nakamura, S, Iwai, T, Honkura, R 1998Complete nucleotide sequence and genome organization of broad bean wilt virus 2.Ann Phytopathol Soc Jpn64565568Google Scholar
  20. Nevins, JR 1983The pathway of eukaryotic messenger RNA formation.Annu Rev Biochem52441466CrossRefPubMedGoogle Scholar
  21. Qi, Y, Zhou, X, Li, D 2000Complete nucleotide sequence and infectious cDNA clone of the RNA1 of a Chinese isolate of broad bean wilt virus 2.Virus Genes20201207CrossRefPubMedGoogle Scholar
  22. Qi, Y, Zhou, X, Xue, C, Li, D 2000Nucleotide sequence of RNA2 and polyprotein processing sites of a Chinese isolate of broad bean wilt virus 2.Prog Nat Sci10680686Google Scholar
  23. Rubio, L, Luis-Arteaga, M, Cambra, M, Serra, J, Moreno, P, Guerri, J 2002First Report of Broad bean wilt virus 1 in Spain.Plant Dis86698Google Scholar
  24. Thompson, JD, Higgins, DG, Gibson, TJ 1994CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice.Nucleic Acids Res2246734680PubMedGoogle Scholar
  25. Vives, MC, Rubio, L, López, C, Navas-Castillo, J, Albiach-Martí, MR, Dawson, WO, Guerri, J, Flores, R, Moreno, P 1999The complete genome sequence of the major component of a mild citrus tristeza virus isolate.J Gen Virol80811816PubMedGoogle Scholar
  26. Vos, P, Verver, J, Jaegle, M, Wellink, J, van Kammen, A, Goldbach, R 1988Two viral proteins involved in the proteolytic processing of the Cowpea mosaic virus polyproteins.Nucleic Acids Res1619671985PubMedGoogle Scholar
  27. Wellink J, Le Gall O, Sanfacon H, Ikegami M, Jones AT (2000) Family Comoviridae. In: van Regenmortel MHV, Fauquet CM, Bishop DHL, Carstens EB, Estes MK, Lemon SM, Maniloff J, Mayo MA, McGeoch DJ, Pringle CR, Wickner RB (eds), Virus taxonomy. Seventh Report of the International Comittee on Taxonomy of Viruses. Academic Press, pp 695–697Google Scholar
  28. Yanagisawa, S, Schmidt, RJ 1999Diversity and similarity among recognition sequences of Dof transcription factors.Plant J17209214CrossRefPubMedGoogle Scholar
  29. Zuker, M 2003Mfold web server for nucleic acid folding and hybridization prediction.Nucleic Acids Res3134063415CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag/Wien 2005

Authors and Affiliations

  • R. M. Ferrer
    • 1
  • J. Guerri
    • 1
  • M. S. Luis-Arteaga
    • 2
  • P. Moreno
    • 1
  • L. Rubio
    • 1
  1. 1.Instituto Valenciano de Investigaciones Agrarias (IVIA)MoncadaSpain
  2. 2.Servicio de Investigación Agroalimentaria (SIA)ZaragozaSpain

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