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Archives of Virology

, Volume 150, Issue 8, pp 1669–1675 | Cite as

Biolistic infection of cassava using cloned components of Indian cassava mosaic virus

  • D. Rothenstein
  • R. W. Briddon
  • D. Haible
  • J. Stanley
  • T. Frischmuth
  • H. Jeske
Brief Report

Summary.

Cassava mosaic disease (CMD) is a major constraint to cassava production in Africa and Asia. Of the two begomoviruses associated with CMD on the Indian subcontinent, Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus, only the latter has been successfully reintroduced into cassava to resolve the aetiology of the disease. Here, we report the complete nucleotide sequence of an ICMV isolate from Maharashtra (ICMV-[Mah2]), central India. Biolistic inoculation of the cloned components produced a systemic infection and typical mosaic symptoms in cassava, thereby fulfilling Koch’s postulates. The availability of infectious clones will provide a valuable tool to screen new cassava cultivars for disease resistance under defined conditions.

Keywords

Nucleotide Infectious Disease Nucleotide Sequence Disease Resistance Mosaic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2005

Authors and Affiliations

  • D. Rothenstein
    • 1
  • R. W. Briddon
    • 2
  • D. Haible
    • 1
  • J. Stanley
    • 2
  • T. Frischmuth
    • 1
  • H. Jeske
    • 1
  1. 1.Department of Molecular Biology and Plant VirologyUniversity of StuttgartStuttgartGermany
  2. 2.Department of Disease and Stress BiologyJohn Innes CentreNorwichU.K.

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