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Archives of Virology

, Volume 150, Issue 7, pp 1449–1458 | Cite as

Human endothelial cells allow passage of an archetypal BK virus (BKV) strain – a tool for cultivation and functional studies of natural BKV strains

  • C. Hanssen Rinaldo
  • H. Hansen
  • T. Traavik
Brief Report

Summary.

The ubiquitous human polyomavirus BK (BKV) causes the serious condition BKV-nephropathy in an increasing number of renal-transplant patients. The lack of authentic cell cultures for multiplication of naturally occurring strains has hampered cultivation and functional studies of BKV.

Here we demonstrate that the most common urine shed BKV strain, the archetype, multiplies in the human endothelial cell line HUV-EC-C. Additional variants with deletions in the non-coding control region (NCCR) appear upon prolonged propagation. Although the titer produced was low, at the present HUV-EC-C is the only cell line shown to allow propagation of archetypal BKV. HUV-EC-C may therefore be a useful tool for BKV cultivation as well as functional studies.

Keywords

Endothelial Cell Cell Culture Infectious Disease Control Region Functional Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2005

Authors and Affiliations

  • C. Hanssen Rinaldo
    • 1
  • H. Hansen
    • 1
  • T. Traavik
    • 1
    • 2
  1. 1.Department of Microbiology and Virology, Faculty of MedicineUniversity of TromsøTromsøNorway
  2. 2.Norwegian Institute of Gene EcologyTromsøNorway

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