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Archives of Virology

, Volume 150, Issue 4, pp 805–814 | Cite as

Differentiation of foot-and-mouth disease virus infected animals from vaccinated animals using a blocking ELISA based on baculovirus expressed FMDV 3ABC antigen and a 3ABC monoclonal antibody

  • K. J. Sørensen
  • K. de Stricker
  • K. C. Dyrting
  • S. Grazioli
  • B. Haas
Brief Report

Summary.

A blocking ELISA that differentiated foot-and-mouth disease virus (FMDV) infected animals from vaccinated animals was developed which uses baculovirus expressed FMDV 3ABC non-structural protein as antigen and monoclonal antibody against FMDV 3ABC non-structural protein as capture and detector antibody. Sera from naive, vaccinated and infected cattle, sheep and pigs were examined. The specificity of the test was high. Non-specific reactions observed in particular in sera of cattle and sheep could be removed by filtration and inactivation. Positive reactions were obtained for sera from cattle infected with all seven serotypes of FMDV. The test detected antibodies from days 7 or 9 following experimental infection of non-vaccinated cattle and sheep, and in cattle strong positive reactions persisted for up to 395 days after infection. In vaccinated cattle that became carriers after challenge with homologous FMDV, positive reactions were obtained in all but one case. In some of these cattle the antibody response was detected late in comparison to the non-vaccinated infected cattle. The test gave results that compared favourably with two commercial ELISA’s when used to test sera from cattle, pigs and sheep collected after experimental or natural infection. The blocking ELISA based on recombinant FMDV 3ABC antigen and a monoclonal antibody to 3ABC is a promising tool for FMD control and eradication campaigns, where vaccination has been carried out.

Keywords

Monoclonal Antibody Infectious Disease Antibody Response Disease Virus Positive Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2005

Authors and Affiliations

  • K. J. Sørensen
    • 1
  • K. de Stricker
    • 1
  • K. C. Dyrting
    • 2
  • S. Grazioli
    • 3
  • B. Haas
    • 4
  1. 1.Department of VirologyDanish Institute for Food and Veterinary ResearchLindholmDenmark
  2. 2.Agriculture, Fisheries and Conservation DepartmentCastle Peak Veterinary LaboratoryHong KongChina
  3. 3.Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia RomagnaBresciaItaly
  4. 4.Federal Research Center for Virus Diseases of Animals, Greifswald – Insel RiemsGermany

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