Archives of Virology

, Volume 150, Issue 4, pp 695–708

Detection and characterization of rabies virus in Southern Brazil by PCR amplification and sequencing of the nucleoprotein gene

  • J. Bordignon
  • G. Brasil-dos-Anjos
  • C. R. Bueno
  • J. Salvatiera-Oporto
  • A. M. R. Dávila
  • E. C. Grisard
  • C. R. Zanetti
Article

Summary.

Due to the medical and socio-economical importance of both human and animal rabies infection, several studies have suggested the use of molecular techniques such as RT-PCR and DNA sequencing for diagnosis and phylogenetic studies of the rabies virus. Considering the conservancy of the nucleoprotein (N) gene of the virus, we herein describe a RT-PCR assay for rabies diagnosis and characterization. A total of 75 samples obtained from a variety of animal species in the state of Santa Catarina (SC), Southern Brazil, were comparatively studied by fluorescence antibody test (FAT), mouse inoculation test (MIT), cell infection assay and RT-PCR, which revealed itself to be as sensitive as FAT and MIT and less time-consuming than MIT. Direct sequencing of the 5′ end of the N gene allowed the clustering of the SC samples with samples from the vampire bat-related or sylvatic cycle through comparative sequence analysis.

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Copyright information

© Springer-Verlag/Wien 2004

Authors and Affiliations

  • J. Bordignon
    • 1
  • G. Brasil-dos-Anjos
    • 1
  • C. R. Bueno
    • 1
  • J. Salvatiera-Oporto
    • 2
  • A. M. R. Dávila
    • 3
  • E. C. Grisard
    • 1
  • C. R. Zanetti
    • 1
  1. 1.Departamento de MicrobiologiaImunologia e Parasitologia da Universidade Federal de Santa CatarinaFlorianópolisBrazil
  2. 2.Companhia Integrada de Desenvolvimento Agropecuário de Santa Catarina (CIDASC)São JosáBrazil
  3. 3.Departamento de Bioquímica e Biologia MolecularInstituto Oswaldo Cruz/FIOCRUZRio de JaneiroBrazil

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