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Archives of Virology

, Volume 149, Issue 5, pp 915–927 | Cite as

Identification of GRP 78 (BiP) as a liver cell expressed receptor element for dengue virus serotype 2

  • S. Jindadamrongwech
  • C. Thepparit
  • D. R. Smith
Article

Summary.

This study sought to identify receptor elements for dengue virus serotype 2 on human liver cells (HepG2) using the viral overlay protein binding assay (VOPBA) technique and Mass Spectrometry fingerprinting. A single major and several minor virus binding bands were observed, and mass spectrometry identified a candidate binding protein for the major binding band as GRP 78 (BiP). GRP78 expression on the cell surface was confirmed, and antibodies directed against both the N and C-terminus of GRP 78 (BiP) altered both the binding of the virus to the cell surface as well as the infectivity profile of HepG2 cells in response to dengue serotype 2 infection. GRP 78 (BiP), which has previously been identified as a co-receptor protein for coxsackievirus A9, is the first non-Fc receptor protein identified for the dengue virus, although GRP78 probably functions as part of a receptor complex.

Keywords

Liver Cell HepG2 Cell Human Liver Receptor Protein Receptor Complex 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 2004

Authors and Affiliations

  • S. Jindadamrongwech
    • 1
  • C. Thepparit
    • 1
  • D. R. Smith
    • 1
  1. 1.Molecular Pathology Laboratory, Institute of Molecular Biology and Genetics, Mahidol UniversityNakorn PathomThailand

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